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• W2101915794 abstract "A laboratory study was carried out on 44 sera of typhoid patients (age 15-45 years, 24 females and 20 males) with positive blood culture for S. typhi, 41 sera non typhoid febrile patients (age 16-51 years, 17 females & 24 males) with negative blood culture and negative Widal test, 136 sera of healthy persons as normal controls (age 20-49 years, 95 females & 41 males). The basic principle of the Dot-EIA typhoid test was an indirect Elisa method using nitrocellulose as a solid phase and OMP S. typhi from five locally prevalent phage types as the antigens. Goat anti-human IgM alkaline phosphatase for the Dot-EIA typhoid IgM test and goat antihuman IgG alkaline phosphatase for the Dot-EIA typhoid IgG test were used as conjugates. BCIP/NBT was used as a chromogenic substrate and the results were visible as gray-blue spots on the surface of the nitrocellulose. The Dot-EIA typhoid test could produce results within 3 hours. The result of the Dot-EIA typhoid test showed a good diagnostic value for typhoid fever, a diagnostic sensitivity of 66 % for IgM and 86.4% for IgG and when both were determined (IgM and IgG) the diagnostic sensitivity was 97.7%. The diagnostic specificity was 100% for IgM and 90.24% for IgG and if both were determined (IgM and IgG) the diagnostic specificity was 90.24%. The diagnostic efficiency was 82.35% for IgM and 88.24% for IgG and when both were determined (IgM and IgG) the diagnostic efficiency was 94.12%. The diagnostic positive predictive value was 100% for IgM and 90.48% for IgG and when both were determined (IgM and IgG) the diagnostic positive predictive value was 97.7%. The diagnostic efficiency was 82.35% for IgM and 90.24% for IgG and when both were determined (IgM and IgG) the diagnostic efficiency was 90.24%. The diagnostic positive predictive value was 100% for IgM and 90.48% for IgG and when both were determined (IgM and IgG) the diagnostic positive predictive value was 97.7%. From a practical point of view under field conditions, the performance of the Dot-EIA typhoid IgM and IgG test could be considered as moderately practicable and not very expensive (Rp 5.025,- per test). Thus this test could be performed in laboratories up to small hospitals (C type hospitals). Based on these results, it can be concluded that the Dot-EIA typhoid test has a good clinical validity, a moderate practicability and is not very expensive." @default.
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• W2101915794 date "2004-01-01" @default.
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• W2101915794 title "Dot-EIA Typhoid Test Using OMP Salmonella typhi Local Phage Type Antigen to Support the Diagnosis of Typhoid Fever" @default.
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