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- W2102010095 abstract "This study provided genetic information on a bile salt hydrolase (bsh) of a Lactobacillus plantarum strain of Indian origin, MBUL90. L. plantarum strains were screened by PCR for the determination of the bsh locus in their genome using specific primers. None of the lactobacilli strains produced the expected size of amplicon (∼ 1.0 kb) except L. plantarum strains, which proved the specificity of the primers. The bsh amplicon of L. plantarum MBUL90 was cloned into pDrive vector, and nucleotide sequences were determined. Sequence analysis of bsh genes revealed a high level of similarity within the species of L. plantarum as well as with other species of Lactobacillus. The resulting nucleotide sequence of an ORF of 975 bp encoded a predicted protein of 324 amino acids representing a theoretical molecular mass of 37 kDa with a pI of 4.92. The protein deduced from the complete ORF had high similarity with other Bsh proteins, and four highly conserved amino acid motifs (YFGRNXD, NEXGLXXAGLNF, VXVLTNNPXF, and SXSRFVRXAF) were located around the active site. Genetic data presented in this paper provide a sound foundation for better understanding the genetic diversity of bsh in Lactobacillus genus and may provide a new genetic marker for phylogenetic study." @default.
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- W2102010095 date "2010-08-31" @default.
- W2102010095 modified "2023-09-25" @default.
- W2102010095 title "Molecular Cloning and Sequence Analysis of Bile Salt Hydrolase Gene (bsh) fromLactobacillus plantarumMBUL90 Strain of Human Origin" @default.
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- W2102010095 doi "https://doi.org/10.1080/08905436.2010.507130" @default.
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