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• W2102023384 abstract "To develop an analytical system for single-nucleotide polymorphisms (SNPs), the fluorescence resonance energy transfer (FRET) technique was employed on a bacterial magnetic particle (BMP) surface. A combination of fluorescein isothiocyanate (FITC; excitation 490 nm/emission 520 nm) labeled at the 5' end of DNA and an intercalating compound (POPO-3, excitation 534 nm/emission 570 nm) was used to avoid the interference from light scattering caused by nanoparticles. After hybridization between target DNA immobilized onto BMPs and FITC-labeled probes, fluorescence from POPO-3, which was excited by the energy from the FITC, was detected. The major homozygous (ALDH2*1), heterozygous (ALDH2*1/*2), and minor homozygous (ALDH2*2) genotypes in the blood samples were discriminated by this method. The assay described herein allows for a simple and rapid SNP analysis using a fully automated system." @default.
• W2102023384 created "2016-06-24" @default.
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• W2102023384 creator A5086989656 @default.
• W2102023384 date "2003-08-05" @default.
• W2102023384 modified "2023-09-26" @default.
• W2102023384 title "Single-nucleotide polymorphism analysis using fluorescence resonance energy transfer between DNA-labeling fluorophore, fluorescein isothiocyanate, and DNA intercalator, POPO-3, on bacterial magnetic particles" @default.
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• W2102023384 doi "https://doi.org/10.1002/bit.10755" @default.
• W2102023384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12910548" @default.
• W2102023384 hasPublicationYear "2003" @default.
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