FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2102147145> ?p ?o ?g. }

• W2102147145 endingPage "e81" @default.
• W2102147145 startingPage "e81" @default.
• W2102147145 abstract "Rolling-circle amplification (RCA) and ramification amplification (RAM, also known as hyperbranched RCA) are isothermal nucleic acid amplification technologies that have gained a great application in in situ signal amplification, DNA and protein microarray assays, single nucleotide polymorphism detection, as well as clinical diagnosis. Real-time detection of RCA or RAM products has been a challenge because of most real-time detection systems, including Taqman and Molecular Beacon, are designed for thermal cycling-based DNA amplification technology. In the present study, we describe a novel fluorescent probe construct, termed molecular zipper, which is specially designed for quantifying target DNA by real-time monitoring RAM reactions. Our results showed that the molecular zipper has very low background fluorescence due to the strong interaction between two strands. Once it is incorporated into the RAM products its double strand region is opened by displacement, therefore, its fluorophore releases a fluorescent signal. Applying the molecular zipper in RAM assay, we were able to detect as few as 10 molecules within 90 min reaction. A linear relationship was observed between initial input of targets and threshold time (R2 = 0.985). These results indicate that molecular zipper can be applied to real-time monitoring and qualification of RAM reaction, implying an amenable method for automatic RAM-based diagnostic assays." @default.
• W2102147145 created "2016-06-24" @default.
• W2102147145 creator A5016320004 @default.
• W2102147145 creator A5052437531 @default.
• W2102147145 creator A5085713254 @default.
• W2102147145 date "2006-06-28" @default.
• W2102147145 modified "2023-10-18" @default.
• W2102147145 title "Molecular Zipper: a fluorescent probe for real-time isothermal DNA amplification" @default.
• W2102147145 cites W1520883967 @default.
• W2102147145 cites W1551009089 @default.
• W2102147145 cites W1649049673 @default.
• W2102147145 cites W1965169728 @default.
• W2102147145 cites W1970586100 @default.
• W2102147145 cites W2010974451 @default.
• W2102147145 cites W2011184654 @default.
• W2102147145 cites W2016084390 @default.
• W2102147145 cites W2026710381 @default.
• W2102147145 cites W2029212995 @default.
• W2102147145 cites W2032118018 @default.
• W2102147145 cites W2032467585 @default.
• W2102147145 cites W2035792726 @default.
• W2102147145 cites W2038311101 @default.
• W2102147145 cites W2047196913 @default.
• W2102147145 cites W2047658079 @default.
• W2102147145 cites W2060094203 @default.
• W2102147145 cites W2065037359 @default.
• W2102147145 cites W2066789848 @default.
• W2102147145 cites W2081325029 @default.
• W2102147145 cites W2105275554 @default.
• W2102147145 cites W2106745559 @default.
• W2102147145 cites W2122374036 @default.
• W2102147145 cites W2141627564 @default.
• W2102147145 cites W2142162336 @default.
• W2102147145 cites W2164578725 @default.
• W2102147145 cites W2165504726 @default.
• W2102147145 cites W2167919140 @default.
• W2102147145 doi "https://doi.org/10.1093/nar/gkl261" @default.
• W2102147145 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1488881" @default.
• W2102147145 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16822854" @default.
• W2102147145 hasPublicationYear "2006" @default.
• W2102147145 type Work @default.
• W2102147145 sameAs 2102147145 @default.
• W2102147145 citedByCount "48" @default.
• W2102147145 countsByYear W21021471452012 @default.
• W2102147145 countsByYear W21021471452013 @default.
• W2102147145 countsByYear W21021471452014 @default.
• W2102147145 countsByYear W21021471452015 @default.
• W2102147145 countsByYear W21021471452016 @default.
• W2102147145 countsByYear W21021471452018 @default.
• W2102147145 countsByYear W21021471452019 @default.
• W2102147145 countsByYear W21021471452020 @default.
• W2102147145 countsByYear W21021471452021 @default.
• W2102147145 countsByYear W21021471452022 @default.
• W2102147145 crossrefType "journal-article" @default.
• W2102147145 hasAuthorship W2102147145A5016320004 @default.
• W2102147145 hasAuthorship W2102147145A5052437531 @default.
• W2102147145 hasAuthorship W2102147145A5085713254 @default.
• W2102147145 hasBestOaLocation W21021471451 @default.
• W2102147145 hasConcept C104317684 @default.
• W2102147145 hasConcept C105782903 @default.
• W2102147145 hasConcept C11413529 @default.
• W2102147145 hasConcept C119203544 @default.
• W2102147145 hasConcept C121332964 @default.
• W2102147145 hasConcept C12554922 @default.
• W2102147145 hasConcept C129312508 @default.
• W2102147145 hasConcept C143660080 @default.
• W2102147145 hasConcept C153911025 @default.
• W2102147145 hasConcept C156860981 @default.
• W2102147145 hasConcept C24107716 @default.
• W2102147145 hasConcept C2778617687 @default.
• W2102147145 hasConcept C41008148 @default.
• W2102147145 hasConcept C48023723 @default.
• W2102147145 hasConcept C54355233 @default.
• W2102147145 hasConcept C552990157 @default.
• W2102147145 hasConcept C60635243 @default.
• W2102147145 hasConcept C62520636 @default.
• W2102147145 hasConcept C70721500 @default.
• W2102147145 hasConcept C86339819 @default.
• W2102147145 hasConcept C86803240 @default.
• W2102147145 hasConcept C91881484 @default.
• W2102147145 hasConceptScore W2102147145C104317684 @default.
• W2102147145 hasConceptScore W2102147145C105782903 @default.
• W2102147145 hasConceptScore W2102147145C11413529 @default.
• W2102147145 hasConceptScore W2102147145C119203544 @default.
• W2102147145 hasConceptScore W2102147145C121332964 @default.
• W2102147145 hasConceptScore W2102147145C12554922 @default.
• W2102147145 hasConceptScore W2102147145C129312508 @default.
• W2102147145 hasConceptScore W2102147145C143660080 @default.
• W2102147145 hasConceptScore W2102147145C153911025 @default.
• W2102147145 hasConceptScore W2102147145C156860981 @default.
• W2102147145 hasConceptScore W2102147145C24107716 @default.
• W2102147145 hasConceptScore W2102147145C2778617687 @default.
• W2102147145 hasConceptScore W2102147145C41008148 @default.
• W2102147145 hasConceptScore W2102147145C48023723 @default.
• W2102147145 hasConceptScore W2102147145C54355233 @default.
• W2102147145 hasConceptScore W2102147145C552990157 @default.
• W2102147145 hasConceptScore W2102147145C60635243 @default.
• W2102147145 hasConceptScore W2102147145C62520636 @default.

• next