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- W2102222071 endingPage "2023" @default.
- W2102222071 startingPage "2007" @default.
- W2102222071 abstract "Abstract Hexaploid bread wheat (Triticum aestivum L. em. Thell) is one of the world's most important crop plants and displays a very low level of intraspecific polymorphism. We report the development of highly polymorphic microsatellite markers using procedures optimized for the large wheat genome. The isolation of microsatellite-containing clones from hypomethylated regions of the wheat genome increased the proportion of useful markers almost twofold. The majority (80%) of primer sets developed are genome-specific and detect only a single locus in one of the three genomes of bread wheat (A, B, or D). Only 20% of the markers detect more than one locus. A total of 279 loci amplified by 230 primer sets were placed onto a genetic framework map composed of RFLPs previously mapped in the reference population of the International Triticeae Mapping Initiative (ITMI) Opata 85 × W7984. Sixty-five microsatellites were mapped at a LOD >2.5, and 214 microsatellites were assigned to the most likely intervals. Ninety-three loci were mapped to the A genome, 115 to the B genome, and 71 to the D genome. The markers are randomly distributed along the linkage map, with clustering in several centromeric regions." @default.
- W2102222071 created "2016-06-24" @default.
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- W2102222071 date "1998-08-01" @default.
- W2102222071 modified "2023-10-15" @default.
- W2102222071 title "A Microsatellite Map of Wheat" @default.
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- W2102222071 doi "https://doi.org/10.1093/genetics/149.4.2007" @default.
- W2102222071 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1460256" @default.
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