FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2102353345> ?p ?o ?g. }

• W2102353345 endingPage "477" @default.
• W2102353345 startingPage "462" @default.
• W2102353345 abstract "Abstract Uroplakin II (UPII) gene expression is highly tissue and cell specific, with mRNA present in the suprabasal cell layers of the bladder and urethra. Previous reports described the mouse UPII (mUPII) promoter as primarily urothelium selective. However, ectopic expression of a transgene under the 3.6 kb mUPII promoter was also detected in brain, kidney, and testis in some transgenic mouse lines. Here, we have cloned an 8.8 kb pig UPII (pUPII) promoter region and investigated which cells within the bladder and urethra express a transgene consisting of the pUPII promoter fused to human erythropoietin (hEPO) or a luciferase gene. pUPII‐luciferase expression vectors with various deletions of the promoter region were introduced into mouse fibroblast (NIH3T3), Chinese hamster ovary (CHO), and human bladder transitional carcinoma (RT4). A 2.1 kb pUPII promoter fragment displayed high levels of luciferase activity in transiently transfected RT4 cells, whereas the 8.8 kb pUPII promoter region displayed only low levels of activity. The pUPII‐hEPO expression vector was injected into the pronucleus of zygotes to make transgenic mice. To elucidate the in vivo molecular mechanisms controlling the tissue‐ and cell‐specific expression of the pUPII promoter gene, transgenic mice containing 2.1 and 8.8 kb pUPII promoter fragments linked to the genomic hEPO gene were generated. An erythropoietin (EPO) assay showed that all nine transgenic lines carrying the 8.8 kb construct expressed recombinant human erythropoietin (rhEPO) only in their urethra and bladder, whereas two transgenic lines carrying the 2.1 kb pUPII promoter displayed hEPO expression in several organs including bladder, kidney, spleen, heart, and brain. These studies demonstrate that the 2.1 kb promoter contains the DNA elements necessary for high levels of expression, but lacks critical sequences necessary for tissue‐specific expression. We compared binding sites in the 2.1 and 8.8 kb promoter sequences and found five peroxisome proliferator responsive elements (PPREs) in the 8.8 kb promoter. Our data demonstrated that proliferator‐activated receptor (PPAR)‐γ activator treatment in RT4 cells induced the elevated expression of hEPO mRNA under the control of the 8.8 kb pUPII promoter, but not the 2.1 kb promoter. Collectively, our data suggested that all the major trans‐regulatory elements required for bladder‐ and urethra‐specific transcription are located in the 8.8 kb upstream region and that it may enhance tissue‐specific protein production and be of interest to clinicians who are searching for therapeutic modalities with high efficacy and low toxicity. J. Cell. Biochem. 99: 462–477, 2006. © 2006 Wiley‐Liss, Inc." @default.
• W2102353345 created "2016-06-24" @default.
• W2102353345 creator A5001188270 @default.
• W2102353345 creator A5023194047 @default.
• W2102353345 creator A5042886121 @default.
• W2102353345 creator A5056692355 @default.
• W2102353345 creator A5074730085 @default.
• W2102353345 creator A5080049203 @default.
• W2102353345 creator A5090821741 @default.
• W2102353345 date "2006-04-17" @default.
• W2102353345 modified "2023-10-17" @default.
• W2102353345 title "Cloning and molecular dissection of the 8.8 kb pig uroplakin II promoter using transgenic mice and RT4 cells" @default.
• W2102353345 cites W1484551801 @default.
• W2102353345 cites W1539409018 @default.
• W2102353345 cites W1812285428 @default.
• W2102353345 cites W1964918240 @default.
• W2102353345 cites W1967055346 @default.
• W2102353345 cites W1968870704 @default.
• W2102353345 cites W1977922844 @default.
• W2102353345 cites W1978125779 @default.
• W2102353345 cites W1978836807 @default.
• W2102353345 cites W1979761686 @default.
• W2102353345 cites W1981946836 @default.
• W2102353345 cites W1988792958 @default.
• W2102353345 cites W1994937581 @default.
• W2102353345 cites W1999453621 @default.
• W2102353345 cites W2001177059 @default.
• W2102353345 cites W2004923742 @default.
• W2102353345 cites W2050649204 @default.
• W2102353345 cites W2054556235 @default.
• W2102353345 cites W2055043387 @default.
• W2102353345 cites W2055184396 @default.
• W2102353345 cites W2064427602 @default.
• W2102353345 cites W2080726666 @default.
• W2102353345 cites W2090122012 @default.
• W2102353345 cites W2093929964 @default.
• W2102353345 cites W2095208510 @default.
• W2102353345 cites W2101287056 @default.
• W2102353345 cites W2119646069 @default.
• W2102353345 cites W2139412202 @default.
• W2102353345 cites W2165089183 @default.
• W2102353345 cites W2165176916 @default.
• W2102353345 cites W2165502699 @default.
• W2102353345 cites W2165547784 @default.
• W2102353345 cites W2210159858 @default.
• W2102353345 cites W2318019606 @default.
• W2102353345 cites W311562328 @default.
• W2102353345 cites W4211065193 @default.
• W2102353345 cites W4327849160 @default.
• W2102353345 doi "https://doi.org/10.1002/jcb.20931" @default.
• W2102353345 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16619260" @default.
• W2102353345 hasPublicationYear "2006" @default.
• W2102353345 type Work @default.
• W2102353345 sameAs 2102353345 @default.
• W2102353345 citedByCount "14" @default.
• W2102353345 countsByYear W21023533452012 @default.
• W2102353345 countsByYear W21023533452013 @default.
• W2102353345 countsByYear W21023533452015 @default.
• W2102353345 countsByYear W21023533452018 @default.
• W2102353345 crossrefType "journal-article" @default.
• W2102353345 hasAuthorship W2102353345A5001188270 @default.
• W2102353345 hasAuthorship W2102353345A5023194047 @default.
• W2102353345 hasAuthorship W2102353345A5042886121 @default.
• W2102353345 hasAuthorship W2102353345A5056692355 @default.
• W2102353345 hasAuthorship W2102353345A5074730085 @default.
• W2102353345 hasAuthorship W2102353345A5080049203 @default.
• W2102353345 hasAuthorship W2102353345A5090821741 @default.
• W2102353345 hasConcept C101762097 @default.
• W2102353345 hasConcept C102230213 @default.
• W2102353345 hasConcept C104317684 @default.
• W2102353345 hasConcept C111335760 @default.
• W2102353345 hasConcept C141035611 @default.
• W2102353345 hasConcept C150194340 @default.
• W2102353345 hasConcept C153911025 @default.
• W2102353345 hasConcept C175656101 @default.
• W2102353345 hasConcept C54009773 @default.
• W2102353345 hasConcept C54355233 @default.
• W2102353345 hasConcept C81885089 @default.
• W2102353345 hasConcept C86803240 @default.
• W2102353345 hasConceptScore W2102353345C101762097 @default.
• W2102353345 hasConceptScore W2102353345C102230213 @default.
• W2102353345 hasConceptScore W2102353345C104317684 @default.
• W2102353345 hasConceptScore W2102353345C111335760 @default.
• W2102353345 hasConceptScore W2102353345C141035611 @default.
• W2102353345 hasConceptScore W2102353345C150194340 @default.
• W2102353345 hasConceptScore W2102353345C153911025 @default.
• W2102353345 hasConceptScore W2102353345C175656101 @default.
• W2102353345 hasConceptScore W2102353345C54009773 @default.
• W2102353345 hasConceptScore W2102353345C54355233 @default.
• W2102353345 hasConceptScore W2102353345C81885089 @default.
• W2102353345 hasConceptScore W2102353345C86803240 @default.
• W2102353345 hasIssue "2" @default.
• W2102353345 hasLocation W21023533451 @default.
• W2102353345 hasLocation W21023533452 @default.
• W2102353345 hasOpenAccess W2102353345 @default.
• W2102353345 hasPrimaryLocation W21023533451 @default.
• W2102353345 hasRelatedWork W1973459962 @default.
• W2102353345 hasRelatedWork W2011995915 @default.

• next