FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2102394165> ?p ?o ?g. }

• W2102394165 endingPage "56" @default.
• W2102394165 startingPage "247" @default.
• W2102394165 abstract "Signaling molecules that are responsible for proliferation and differentiation of hematopoietic cells following ectopic expression of receptor tyrosine kinases (RTKs) were investigated in the interleukin 3 (IL-3)-dependent hematopoietic cell line, FDC-P1. Cells were transfected with human platelet-derived growth factor receptor (PDGF-R), macrophage colony stimulating factor-1 receptor (CSF-1R), epidermal growth factor receptor (EGF-R), and chimeras consisting of the extracellular domain of EGF-R and the transmembrane and cytoplasmic domains of either HER2 (HER1-2) or c-kit (EK-R). All FDC-P1 transfectants proliferated in response to the corresponding growth factor in the absence of IL-3. However, only cells expressing PDGF-R, CSF-1R, and EK-R (type III RTKs) differentiated along the monocyte-macrophage lineage after treatment with their activating ligands. Analysis of proteins from these RTK-expressing cells revealed that a Mr 85,000 protein showed in vitro phosphorylation, and V8 protease peptide mapping showed that this protein was p85, the regulatory subunit of phosphatidylinositol 3-kinase (PI3-kinase). Accordingly, activation of PDGF-R-, CSF-1R-, and EK-R-expressing cells led to an increase in PI3-kinase activity. Expression of EK-R mutant Y721F, which lacked the known p85 binding site, blocked differentiation and activation of PI3-kinase, without affecting proliferation. Last, addition of wortmannin to cells expressing PDGF-R, CSF-1R, and EK-R blocked ligand-induced differentiation in a concentration-dependent manner, and this effect correlated with wortmannin's ability to inhibit PI3-kinase. Thus, ectopic expression of both type I and III RTKs could stimulate FDC-P1 proliferation in the absence of IL-3; however, only activation of type III RTKs led to differentiation via selective coupling to p85 and PI3-kinase activation." @default.
• W2102394165 created "2016-06-24" @default.
• W2102394165 creator A5016538112 @default.
• W2102394165 creator A5017832747 @default.
• W2102394165 creator A5064913004 @default.
• W2102394165 creator A5085976480 @default.
• W2102394165 creator A5090328045 @default.
• W2102394165 date "1998-03-01" @default.
• W2102394165 modified "2023-10-18" @default.
• W2102394165 title "Activation of phosphatidylinositol 3-kinase is necessary for differentiation of FDC-P1 cells following stimulation of type III receptor tyrosine kinases." @default.
• W2102394165 cites W1428006903 @default.
• W2102394165 cites W1489462130 @default.
• W2102394165 cites W1547466369 @default.
• W2102394165 cites W1574657504 @default.
• W2102394165 cites W1664836047 @default.
• W2102394165 cites W1678269499 @default.
• W2102394165 cites W1750539994 @default.
• W2102394165 cites W1800125776 @default.
• W2102394165 cites W1810032586 @default.
• W2102394165 cites W1876455218 @default.
• W2102394165 cites W1892433039 @default.
• W2102394165 cites W1920844816 @default.
• W2102394165 cites W1931293178 @default.
• W2102394165 cites W193772436 @default.
• W2102394165 cites W1966018982 @default.
• W2102394165 cites W1967882942 @default.
• W2102394165 cites W1978023859 @default.
• W2102394165 cites W1988900127 @default.
• W2102394165 cites W1993733425 @default.
• W2102394165 cites W1996728737 @default.
• W2102394165 cites W1997257161 @default.
• W2102394165 cites W1998087920 @default.
• W2102394165 cites W1999824494 @default.
• W2102394165 cites W2009857536 @default.
• W2102394165 cites W2020953630 @default.
• W2102394165 cites W2032359283 @default.
• W2102394165 cites W2041102071 @default.
• W2102394165 cites W2045177756 @default.
• W2102394165 cites W2060280494 @default.
• W2102394165 cites W2063117678 @default.
• W2102394165 cites W2082743073 @default.
• W2102394165 cites W2096199069 @default.
• W2102394165 cites W2100638594 @default.
• W2102394165 cites W2103043702 @default.
• W2102394165 cites W2140524461 @default.
• W2102394165 cites W2150123903 @default.
• W2102394165 cites W2163061849 @default.
• W2102394165 cites W2164358316 @default.
• W2102394165 cites W2253820759 @default.
• W2102394165 cites W2261356028 @default.
• W2102394165 cites W2305244369 @default.
• W2102394165 cites W2402910955 @default.
• W2102394165 cites W2403882570 @default.
• W2102394165 cites W2411312370 @default.
• W2102394165 cites W248614516 @default.
• W2102394165 cites W28625760 @default.
• W2102394165 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9543391" @default.
• W2102394165 hasPublicationYear "1998" @default.
• W2102394165 type Work @default.
• W2102394165 sameAs 2102394165 @default.
• W2102394165 citedByCount "14" @default.
• W2102394165 crossrefType "journal-article" @default.
• W2102394165 hasAuthorship W2102394165A5016538112 @default.
• W2102394165 hasAuthorship W2102394165A5017832747 @default.
• W2102394165 hasAuthorship W2102394165A5064913004 @default.
• W2102394165 hasAuthorship W2102394165A5085976480 @default.
• W2102394165 hasAuthorship W2102394165A5090328045 @default.
• W2102394165 hasConcept C101544691 @default.
• W2102394165 hasConcept C153911025 @default.
• W2102394165 hasConcept C170493617 @default.
• W2102394165 hasConcept C180361614 @default.
• W2102394165 hasConcept C184235292 @default.
• W2102394165 hasConcept C26375932 @default.
• W2102394165 hasConcept C2775960820 @default.
• W2102394165 hasConcept C2776362946 @default.
• W2102394165 hasConcept C2777553839 @default.
• W2102394165 hasConcept C2778308172 @default.
• W2102394165 hasConcept C2780610907 @default.
• W2102394165 hasConcept C42362537 @default.
• W2102394165 hasConcept C55493867 @default.
• W2102394165 hasConcept C62478195 @default.
• W2102394165 hasConcept C86803240 @default.
• W2102394165 hasConcept C95444343 @default.
• W2102394165 hasConceptScore W2102394165C101544691 @default.
• W2102394165 hasConceptScore W2102394165C153911025 @default.
• W2102394165 hasConceptScore W2102394165C170493617 @default.
• W2102394165 hasConceptScore W2102394165C180361614 @default.
• W2102394165 hasConceptScore W2102394165C184235292 @default.
• W2102394165 hasConceptScore W2102394165C26375932 @default.
• W2102394165 hasConceptScore W2102394165C2775960820 @default.
• W2102394165 hasConceptScore W2102394165C2776362946 @default.
• W2102394165 hasConceptScore W2102394165C2777553839 @default.
• W2102394165 hasConceptScore W2102394165C2778308172 @default.
• W2102394165 hasConceptScore W2102394165C2780610907 @default.
• W2102394165 hasConceptScore W2102394165C42362537 @default.
• W2102394165 hasConceptScore W2102394165C55493867 @default.
• W2102394165 hasConceptScore W2102394165C62478195 @default.
• W2102394165 hasConceptScore W2102394165C86803240 @default.

• next