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• W2102411651 endingPage "3783" @default.
• W2102411651 startingPage "3777" @default.
• W2102411651 abstract "ABSTRACT Stx2d is a recently described Shiga toxin whose cytotoxicity is activated 10- to 1,000-fold by the elastase present in mouse or human intestinal mucus. We examined Shiga toxigenic Escherichia coli (STEC) strains isolated from food and livestock sources for the presence of activatable stx 2d . The stx 2 operons of STEC were first analyzed by PCR-restriction fragment length polymorphism (RFLP) analysis and categorized as stx 2 , stx 2c vha , stx 2c vhb , or stx 2d EH250 . Subsequently, the stx 2c vha and stx 2c vhb operons were screened for the absence of a Pst I site in the stx 2A subunit gene, a restriction site polymorphism which is a predictive indicator for the stx 2d (activatable) genotype. Twelve STEC isolates carrying putative stx 2d operons were identified, and nucleotide sequencing was used to confirm the identification of these operons as stx 2d . The complete nucleotide sequences of seven representative stx 2d operons were determined. Shiga toxin expression in stx 2d isolates was confirmed by immunoblotting. stx 2d isolates were induced for the production of bacteriophages carrying stx . Two isolates were able to produce bacteriophages φ1662a and φ1720a carrying the stx 2d operons. RFLP analysis of bacteriophage genomic DNA revealed that φ1662a and φ1720a were highly related to each other; however, the DNA sequences of these two stx 2d operons were distinct. The STEC strains carrying these operons were isolated from retail ground beef. Surveillance for STEC strains expressing activatable Stx2d Shiga toxin among clinical cases may indicate the significance of this toxin subtype to human health." @default.
• W2102411651 created "2016-06-24" @default.
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• W2102411651 creator A5028015938 @default.
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• W2102411651 date "2003-08-01" @default.
• W2102411651 modified "2023-09-26" @default.
• W2102411651 title "Presence of Activatable Shiga Toxin Genotype ( <i>stx</i> _2d ) in Shiga Toxigenic <i>Escherichia coli</i> from Livestock Sources" @default.
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• W2102411651 doi "https://doi.org/10.1128/jcm.41.8.3777-3783.2003" @default.
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