FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2102426888> ?p ?o ?g. }

• W2102426888 endingPage "6797" @default.
• W2102426888 startingPage "6789" @default.
• W2102426888 abstract "To probe the potential for activity in unevolved amino acid sequence space, we created a third generation combinatorial library of de novo four-helix bundle proteins. The artificial superfamily of helical bundles was designed using binary patterning of polar and nonpolar residues, and expressed in Escherichia coli from a library of synthetic genes. WA20, picked from the library, is one of the most stable proteins in the superfamily, and has rudimentary activities such as esterase and lipase. Here we report the crystal structure of WA20, determined by the multiwavelength anomalous dispersion method. Unexpectedly, the WA20 crystal structure is not a monomeric four-helix bundle, but a dimeric four-helix bundle. Each monomer comprises two long α-helices that intertwist with the helices of the other monomer. The two monomers together form a 3D domain-swapped four-helix bundle dimer. In addition, there are two hydrophobic pockets, which may potentially provide substrate binding sites. Small-angle X-ray scattering shows that the molecular weight of WA20 is ~25 kDa and the shape is rod-like (the maximum length, D(max) = ~8 nm), indicating that WA20 forms a dimeric four-helix bundle in solution. These results demonstrate that our de novo protein library contains not only simple monomeric proteins, but also stable and functional multimeric proteins." @default.
• W2102426888 created "2016-06-24" @default.
• W2102426888 creator A5013951672 @default.
• W2102426888 creator A5021195776 @default.
• W2102426888 creator A5021390980 @default.
• W2102426888 creator A5022934494 @default.
• W2102426888 creator A5033527498 @default.
• W2102426888 creator A5036251737 @default.
• W2102426888 creator A5041967476 @default.
• W2102426888 creator A5051037773 @default.
• W2102426888 creator A5079411902 @default.
• W2102426888 date "2012-04-10" @default.
• W2102426888 modified "2023-10-01" @default.
• W2102426888 title "Domain-Swapped Dimeric Structure of a Stable and Functional <i>De Novo</i> Four-Helix Bundle Protein, WA20" @default.
• W2102426888 cites W1539796472 @default.
• W2102426888 cites W1551404386 @default.
• W2102426888 cites W1945269072 @default.
• W2102426888 cites W1965277349 @default.
• W2102426888 cites W1969156185 @default.
• W2102426888 cites W1969644422 @default.
• W2102426888 cites W1973234898 @default.
• W2102426888 cites W1986191025 @default.
• W2102426888 cites W1997886489 @default.
• W2102426888 cites W2000106654 @default.
• W2102426888 cites W2001641653 @default.
• W2102426888 cites W2008866677 @default.
• W2102426888 cites W2013041880 @default.
• W2102426888 cites W2014059004 @default.
• W2102426888 cites W2020610175 @default.
• W2102426888 cites W2022996090 @default.
• W2102426888 cites W2031194866 @default.
• W2102426888 cites W2038840577 @default.
• W2102426888 cites W2039377812 @default.
• W2102426888 cites W2048711222 @default.
• W2102426888 cites W2050229371 @default.
• W2102426888 cites W2063937171 @default.
• W2102426888 cites W2070395446 @default.
• W2102426888 cites W2074986801 @default.
• W2102426888 cites W2076398541 @default.
• W2102426888 cites W2078398728 @default.
• W2102426888 cites W2081912151 @default.
• W2102426888 cites W2085497102 @default.
• W2102426888 cites W2100429271 @default.
• W2102426888 cites W2103169789 @default.
• W2102426888 cites W2114155688 @default.
• W2102426888 cites W2116404881 @default.
• W2102426888 cites W2116441099 @default.
• W2102426888 cites W2117978571 @default.
• W2102426888 cites W2122339645 @default.
• W2102426888 cites W2124026197 @default.
• W2102426888 cites W2154714625 @default.
• W2102426888 cites W2156697496 @default.
• W2102426888 cites W2157455847 @default.
• W2102426888 cites W2159211495 @default.
• W2102426888 cites W2160082320 @default.
• W2102426888 cites W2172219847 @default.
• W2102426888 cites W2409474219 @default.
• W2102426888 doi "https://doi.org/10.1021/jp212438h" @default.
• W2102426888 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22397676" @default.
• W2102426888 hasPublicationYear "2012" @default.
• W2102426888 type Work @default.
• W2102426888 sameAs 2102426888 @default.
• W2102426888 citedByCount "29" @default.
• W2102426888 countsByYear W21024268882012 @default.
• W2102426888 countsByYear W21024268882013 @default.
• W2102426888 countsByYear W21024268882014 @default.
• W2102426888 countsByYear W21024268882015 @default.
• W2102426888 countsByYear W21024268882016 @default.
• W2102426888 countsByYear W21024268882017 @default.
• W2102426888 countsByYear W21024268882018 @default.
• W2102426888 countsByYear W21024268882019 @default.
• W2102426888 countsByYear W21024268882020 @default.
• W2102426888 countsByYear W21024268882021 @default.
• W2102426888 countsByYear W21024268882022 @default.
• W2102426888 countsByYear W21024268882023 @default.
• W2102426888 crossrefType "journal-article" @default.
• W2102426888 hasAuthorship W2102426888A5013951672 @default.
• W2102426888 hasAuthorship W2102426888A5021195776 @default.
• W2102426888 hasAuthorship W2102426888A5021390980 @default.
• W2102426888 hasAuthorship W2102426888A5022934494 @default.
• W2102426888 hasAuthorship W2102426888A5033527498 @default.
• W2102426888 hasAuthorship W2102426888A5036251737 @default.
• W2102426888 hasAuthorship W2102426888A5041967476 @default.
• W2102426888 hasAuthorship W2102426888A5051037773 @default.
• W2102426888 hasAuthorship W2102426888A5079411902 @default.
• W2102426888 hasConcept C166940927 @default.
• W2102426888 hasConcept C178790620 @default.
• W2102426888 hasConcept C185592680 @default.
• W2102426888 hasConcept C18903297 @default.
• W2102426888 hasConcept C203087120 @default.
• W2102426888 hasConcept C2778530040 @default.
• W2102426888 hasConcept C2779546866 @default.
• W2102426888 hasConcept C2779965526 @default.
• W2102426888 hasConcept C47701112 @default.
• W2102426888 hasConcept C521977710 @default.
• W2102426888 hasConcept C55493867 @default.
• W2102426888 hasConcept C71240020 @default.
• W2102426888 hasConcept C8010536 @default.

• next