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- W2102453974 abstract "Lanthanide-based resonance energy transfer (LRET) is an established method for measuring or detecting proximity between a luminescent lanthanide (energy donor) and an organic fluorophore (energy acceptor). Because resonance energy transfer is a distance-dependent phenomenon that increases in efficiency to the 6th power of the distance between the donor and the acceptor, assay systems are often designed to minimize donor-acceptor distances. However, the authors show that because of the R(6) relationship between transfer efficiency and sensitized emission lifetime, energy transfer can be difficult to measure in a time-gated manner when the donor-acceptor distance is small relative to the Förster radius. In such systems, the advantages inherent in time-resolved, ratiometric measurements are lost but can be regained by designing the system such that the average donor-acceptor distance is increased." @default.
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- W2102453974 date "2006-06-01" @default.
- W2102453974 modified "2023-10-11" @default.
- W2102453974 title "Improving Lanthanide-Based Resonance Energy Transfer Detection by Increasing Donor-Acceptor Distances" @default.
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- W2102453974 doi "https://doi.org/10.1177/1087057106287142" @default.
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