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- W2102676182 endingPage "877" @default.
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- W2102676182 abstract "ABSTRACT Staphylococcus aureus is an important human pathogen that is also able to kill the model nematode Caenorhabditis elegans . We constructed a 2,950-member Tn 917 transposon insertion library in S. aureus strain NCTC 8325. Twenty-one of these insertions exhibited attenuated C. elegans killing, and of these, 12 contained insertions in different genes or chromosomal locations. Ten of these 12 insertions showed attenuated killing phenotypes when transduced into two different S. aureus strains, and 5 of the 10 mutants correspond to genes that have not been previously identified in signature-tagged mutagenesis studies. These latter five mutants were tested in a murine renal abscess model, and one mutant harboring an insertion in nagD exhibited attenuated virulence. Interestingly, Tn 917 was shown to have a very strong bias for insertions near the terminus of DNA replication." @default.
- W2102676182 created "2016-06-24" @default.
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- W2102676182 date "2005-02-01" @default.
- W2102676182 modified "2023-09-26" @default.
- W2102676182 title "<i>Staphylococcus aureus</i> Virulence Factors Identified by Using a High-Throughput <i>Caenorhabditis elegans-</i> Killing Model" @default.
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- W2102676182 doi "https://doi.org/10.1128/iai.73.2.872-877.2005" @default.
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