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• W2102690251 abstract "A new cell line was derived from primary culture of rat choroid plexus (RCP) by immortalization with the TSOri minus adenovirus. The selected clone expressed vasopressin V1a receptors at a density of 64,000 sites per cell, and a K(d) of 7.2 nM. Addition of vasopressin to the RCP cells induced a transient calcium peak comparable to V1a receptor signalling in different expression systems. This [Ca(2+)](i) increase was dose-dependent with an EC(50) of 22 nM vasopressin. Similar [Ca(2+)](i) increase was elicited by addition of serotonin, angiotensin II, endothelin-1, and bradykinin. Heterologous desensitization of V1a receptor was observed in RCP cells exposed to the phorbol ester PMA or following stimulation of other receptors coupled to the phosphoinositide pathway. Positive immunolabelling with Factor VIII, Flt1 and CD 34 antibodies suggests that this new RCP cell line originated from endothelial cells of rat choroid plexus." @default.
• W2102690251 created "2016-06-24" @default.
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• W2102690251 date "2000-08-01" @default.
• W2102690251 modified "2023-10-18" @default.
• W2102690251 title "Vasopressin V1a Receptor Signaling in a Rat Choroid Plexus Cell Line" @default.
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• W2102690251 doi "https://doi.org/10.1006/bbrc.2000.3301" @default.
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