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- W2102757992 abstract "The composition of membranes from whole cells and minicells of Bacillus subtilis strain BD474 was examined. Two-dimensional electrophoresis showed that the content of several individual polypeptides differed in the two membrane types. Whole cell membranes appeared to contain more penicillin binding protein (PBP) 2a, glycerol-3-phosphate dehydrogenase, succinate dehydrogenase and NADH dehydrogenase than minicell membranes, but less PBP2b, lactate dehydrogenase and malate dehydrogenase. Glycerol-3-phosphate dehydrogenase and succinate dehydrogenase were unstable, but the other enzymes and PBPs were stable after inhibition of protein synthesis by chloramphenicol. Minicells synthesized peptidoglycan because they incorporated 14C-labelled acetylglucosamine into acid precipitable material. However, the rate of incorporation in minicells was only 5% of the value observed in whole cells. Whole cell and minicell membranes contained the same polar lipids and fatty acids, but the whole cell membranes were enriched in phosphatidylglycerol, diglycosyldiacylglycerol, iso-C15 and anteiso-C15 fatty acids, but depleted of phosphatidylethanolamine, iso-C14, iso-C16, n-C16 and anteiso-C17 fatty acids." @default.
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- W2102757992 date "1985-02-01" @default.
- W2102757992 modified "2023-09-26" @default.
- W2102757992 title "Composition of Membranes from Whole Cells and Minicells of Bacillus subtilis" @default.
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