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- W2102836006 abstract "Albino strain KB20M1 of Monascus purpureus was genetically transformed to hygromycin B resistance with cosmid pMOcosX, using biolistic bombardment. Conidia and mycelial fragments were used as the recipient material and rupture disk strengths of 6.2 and 9.3 MPa each yielded a transformed isolate from five bombardments. Southern analysis suggested a single copy of the cosmid had integrated into each of the transformants. Both of the independent transformants formed cleistothecia but ascospore formation was greatly reduced or absent, suggesting the integration and expression of the genes carried on pMOcosX interfered with fertilization and/or ascospore formation in this homothallic fungus." @default.
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- W2102836006 date "2003-08-15" @default.
- W2102836006 modified "2023-09-26" @default.
- W2102836006 title "Transformation of Monascus purpureus to hygromycin B resistance with cosmid pMOcosX reduces fertility" @default.
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- W2102836006 doi "https://doi.org/10.4067/s0717-34582003000200007" @default.
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