Matches in SemOpenAlex for { <https://semopenalex.org/work/W2102858085> ?p ?o ?g. }
- W2102858085 endingPage "4670" @default.
- W2102858085 startingPage "4656" @default.
- W2102858085 abstract "Sporadic mutations in the hMeCP2 gene, coding for a protein that preferentially binds symmetrically methylated CpGs, result in the severe neurological disorder Rett syndrome (RTT). In the present work, employing a wide range of experimental approaches, we shed new light on the many levels of MeCP2 interaction with DNA and chromatin. We show that strong methylation-independent as well as methylation-dependent binding by MeCP2 is influenced by DNA length. Although MeCP2 is strictly monomeric in solution, its binding to DNA is cooperative, with dimeric binding strongly correlated with methylation density, and strengthened by nearby A/T repeats. Dimeric binding is abolished in the F155S and R294X severe RTT mutants. MeCP2 also binds chromatin in vitro, resulting in compaction-related changes in nucleosome architecture that resemble the classical zigzag motif induced by histone H1 and considered important for 30-nm-fiber formation. In vivo chromatin binding kinetics and in vitro steady-state nucleosome binding of both MeCP2 and H1 provide strong evidence for competition between MeCP2 and H1 for common binding sites. This suggests that chromatin binding by MeCP2 and H1 in vivo should be viewed in the context of competitive multifactorial regulation." @default.
- W2102858085 created "2016-06-24" @default.
- W2102858085 creator A5033373220 @default.
- W2102858085 creator A5053540822 @default.
- W2102858085 creator A5055261141 @default.
- W2102858085 creator A5061335675 @default.
- W2102858085 creator A5088641145 @default.
- W2102858085 date "2010-10-01" @default.
- W2102858085 modified "2023-09-26" @default.
- W2102858085 title "MeCP2 Binds Cooperatively to Its Substrate and Competes with Histone H1 for Chromatin Binding Sites" @default.
- W2102858085 cites W131822264 @default.
- W2102858085 cites W1520946154 @default.
- W2102858085 cites W1559742681 @default.
- W2102858085 cites W1568313174 @default.
- W2102858085 cites W1572088192 @default.
- W2102858085 cites W1632044028 @default.
- W2102858085 cites W1635552303 @default.
- W2102858085 cites W1890119853 @default.
- W2102858085 cites W1967154908 @default.
- W2102858085 cites W1970149098 @default.
- W2102858085 cites W1971284874 @default.
- W2102858085 cites W1975317507 @default.
- W2102858085 cites W1978107331 @default.
- W2102858085 cites W1981831332 @default.
- W2102858085 cites W1981977553 @default.
- W2102858085 cites W1993137394 @default.
- W2102858085 cites W1995470989 @default.
- W2102858085 cites W1997641930 @default.
- W2102858085 cites W2000073535 @default.
- W2102858085 cites W2000342325 @default.
- W2102858085 cites W2001936578 @default.
- W2102858085 cites W2005641629 @default.
- W2102858085 cites W2008753573 @default.
- W2102858085 cites W2009143215 @default.
- W2102858085 cites W2017178787 @default.
- W2102858085 cites W2017314177 @default.
- W2102858085 cites W2018771202 @default.
- W2102858085 cites W2018947009 @default.
- W2102858085 cites W2025919590 @default.
- W2102858085 cites W2029331780 @default.
- W2102858085 cites W2030023744 @default.
- W2102858085 cites W2032151283 @default.
- W2102858085 cites W2032660941 @default.
- W2102858085 cites W2033162367 @default.
- W2102858085 cites W2035090622 @default.
- W2102858085 cites W2038151035 @default.
- W2102858085 cites W2042269850 @default.
- W2102858085 cites W2043676713 @default.
- W2102858085 cites W2044107593 @default.
- W2102858085 cites W2047422719 @default.
- W2102858085 cites W2049545055 @default.
- W2102858085 cites W2052842503 @default.
- W2102858085 cites W2059305083 @default.
- W2102858085 cites W2061764188 @default.
- W2102858085 cites W2062514148 @default.
- W2102858085 cites W2067004457 @default.
- W2102858085 cites W2067267228 @default.
- W2102858085 cites W2068266901 @default.
- W2102858085 cites W2071730442 @default.
- W2102858085 cites W2074302707 @default.
- W2102858085 cites W2075334136 @default.
- W2102858085 cites W2086485143 @default.
- W2102858085 cites W2089191944 @default.
- W2102858085 cites W2097321367 @default.
- W2102858085 cites W2097778167 @default.
- W2102858085 cites W2099351601 @default.
- W2102858085 cites W2103620614 @default.
- W2102858085 cites W2103743266 @default.
- W2102858085 cites W2111592257 @default.
- W2102858085 cites W2113377321 @default.
- W2102858085 cites W2116119112 @default.
- W2102858085 cites W2121003260 @default.
- W2102858085 cites W2121417064 @default.
- W2102858085 cites W2123979543 @default.
- W2102858085 cites W2144792857 @default.
- W2102858085 cites W2145379834 @default.
- W2102858085 cites W2163449711 @default.
- W2102858085 cites W2166633156 @default.
- W2102858085 cites W2167388443 @default.
- W2102858085 cites W2172197806 @default.
- W2102858085 doi "https://doi.org/10.1128/mcb.00379-10" @default.
- W2102858085 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2950531" @default.
- W2102858085 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20679481" @default.
- W2102858085 hasPublicationYear "2010" @default.
- W2102858085 type Work @default.
- W2102858085 sameAs 2102858085 @default.
- W2102858085 citedByCount "114" @default.
- W2102858085 countsByYear W21028580852012 @default.
- W2102858085 countsByYear W21028580852013 @default.
- W2102858085 countsByYear W21028580852014 @default.
- W2102858085 countsByYear W21028580852015 @default.
- W2102858085 countsByYear W21028580852016 @default.
- W2102858085 countsByYear W21028580852017 @default.
- W2102858085 countsByYear W21028580852018 @default.
- W2102858085 countsByYear W21028580852019 @default.
- W2102858085 countsByYear W21028580852020 @default.
- W2102858085 countsByYear W21028580852021 @default.
- W2102858085 countsByYear W21028580852022 @default.