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• W2103019017 abstract "Expression of GFP and other fluorescent proteins depends on cis-regulatory elements. Because these elements rarely direct expression to specific cell types, GFP production cannot always be sufficiently limited. Here we show that reconstitution of GFP, YFP, and CFP previously split into two polypeptides yields fluorescent products when coexpressed in C. elegans. Because this reconstitution involves two components, it can confirm cellular coexpression and identify cells expressing a previously uncharacterized promoter. By choosing promoters whose expression patterns overlap for a single cell type, we can produce animals with fluorescence only in those cells. Furthermore, when one partial GFP polypeptide is fused with a subcellularly localized protein or peptide, this restricted expression leads to the fluorescent marking of cellular components in a subset of cells." @default.
• W2103019017 created "2016-06-24" @default.
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• W2103019017 creator A5065065289 @default.
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• W2103019017 date "2004-10-01" @default.
• W2103019017 modified "2023-10-18" @default.
• W2103019017 title "Combinatorial Marking of Cells and Organelles with Reconstituted Fluorescent Proteins" @default.
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• W2103019017 doi "https://doi.org/10.1016/j.cell.2004.09.012" @default.
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