Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103042813> ?p ?o ?g. }
- W2103042813 endingPage "61" @default.
- W2103042813 startingPage "355" @default.
- W2103042813 abstract "The genetic diagnosis of pathogenic agents using microarrays has the advantage of high-throughput detection, but a relatively large amount of DNA sample is required. To obtain a sufficient amount of DNA for molecular diagnoses, several whole genome amplification (WGA) methods have been proposed. In this study, using Francisella tularensis and Escherichia coli as models, we compared four WGA methods in terms of their efficiency of amplification of whole genomic DNA in order to identify the most suitable method for preparing DNA to be used for microarray analysis. It was possible to obtain more than 1.5 microg of products from 10 ng of F. tularensis and E. coli genomic DNA using four methods, but biases in the amplification of bacterial genes were least prominent in the multiple displacement amplification (MDA) or OmniPlex WGA. When the amplified DNAs were applied to microarray slides consisting of 32 different genes probes, DNAs amplified by Phi29 v2 of MDA and OmniPlex WGA showed high signal intensity as well as a high signal-to-noise ratio for all 32 genes. These results indicate that Phi29 v2 and OmniPlex WGA are useful methods for obtaining sufficient DNA from a limited amount of samples for the detection of microbes using microarrays." @default.
- W2103042813 created "2016-06-24" @default.
- W2103042813 creator A5000320228 @default.
- W2103042813 creator A5022113848 @default.
- W2103042813 creator A5022412527 @default.
- W2103042813 creator A5035054795 @default.
- W2103042813 creator A5041009068 @default.
- W2103042813 creator A5055774707 @default.
- W2103042813 date "2007-11-01" @default.
- W2103042813 modified "2023-09-23" @default.
- W2103042813 title "Comparison of whole genome amplification methods for detecting pathogenic bacterial genomic DNA using microarray." @default.
- W2103042813 cites W1525336418 @default.
- W2103042813 cites W1575228817 @default.
- W2103042813 cites W1576737979 @default.
- W2103042813 cites W1756415316 @default.
- W2103042813 cites W1970980262 @default.
- W2103042813 cites W1971505901 @default.
- W2103042813 cites W1984731778 @default.
- W2103042813 cites W2009930810 @default.
- W2103042813 cites W2010900693 @default.
- W2103042813 cites W2034417112 @default.
- W2103042813 cites W2037142940 @default.
- W2103042813 cites W2037771398 @default.
- W2103042813 cites W2086814185 @default.
- W2103042813 cites W2097097085 @default.
- W2103042813 cites W2097331522 @default.
- W2103042813 cites W2097348296 @default.
- W2103042813 cites W2124919380 @default.
- W2103042813 cites W2126423401 @default.
- W2103042813 cites W2128174087 @default.
- W2103042813 cites W2133122565 @default.
- W2103042813 cites W2146531100 @default.
- W2103042813 cites W2153125376 @default.
- W2103042813 cites W2170939735 @default.
- W2103042813 cites W2338162854 @default.
- W2103042813 cites W2580627695 @default.
- W2103042813 cites W2117130508 @default.
- W2103042813 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18032834" @default.
- W2103042813 hasPublicationYear "2007" @default.
- W2103042813 type Work @default.
- W2103042813 sameAs 2103042813 @default.
- W2103042813 citedByCount "11" @default.
- W2103042813 countsByYear W21030428132013 @default.
- W2103042813 countsByYear W21030428132015 @default.
- W2103042813 countsByYear W21030428132016 @default.
- W2103042813 countsByYear W21030428132017 @default.
- W2103042813 countsByYear W21030428132018 @default.
- W2103042813 countsByYear W21030428132021 @default.
- W2103042813 crossrefType "journal-article" @default.
- W2103042813 hasAuthorship W2103042813A5000320228 @default.
- W2103042813 hasAuthorship W2103042813A5022113848 @default.
- W2103042813 hasAuthorship W2103042813A5022412527 @default.
- W2103042813 hasAuthorship W2103042813A5035054795 @default.
- W2103042813 hasAuthorship W2103042813A5041009068 @default.
- W2103042813 hasAuthorship W2103042813A5055774707 @default.
- W2103042813 hasConcept C104317684 @default.
- W2103042813 hasConcept C141231307 @default.
- W2103042813 hasConcept C150194340 @default.
- W2103042813 hasConcept C153911025 @default.
- W2103042813 hasConcept C156385826 @default.
- W2103042813 hasConcept C17757408 @default.
- W2103042813 hasConcept C186836561 @default.
- W2103042813 hasConcept C2780467176 @default.
- W2103042813 hasConcept C49105822 @default.
- W2103042813 hasConcept C54355233 @default.
- W2103042813 hasConcept C552990157 @default.
- W2103042813 hasConcept C60987743 @default.
- W2103042813 hasConcept C70721500 @default.
- W2103042813 hasConcept C78063203 @default.
- W2103042813 hasConcept C86803240 @default.
- W2103042813 hasConcept C95371953 @default.
- W2103042813 hasConceptScore W2103042813C104317684 @default.
- W2103042813 hasConceptScore W2103042813C141231307 @default.
- W2103042813 hasConceptScore W2103042813C150194340 @default.
- W2103042813 hasConceptScore W2103042813C153911025 @default.
- W2103042813 hasConceptScore W2103042813C156385826 @default.
- W2103042813 hasConceptScore W2103042813C17757408 @default.
- W2103042813 hasConceptScore W2103042813C186836561 @default.
- W2103042813 hasConceptScore W2103042813C2780467176 @default.
- W2103042813 hasConceptScore W2103042813C49105822 @default.
- W2103042813 hasConceptScore W2103042813C54355233 @default.
- W2103042813 hasConceptScore W2103042813C552990157 @default.
- W2103042813 hasConceptScore W2103042813C60987743 @default.
- W2103042813 hasConceptScore W2103042813C70721500 @default.
- W2103042813 hasConceptScore W2103042813C78063203 @default.
- W2103042813 hasConceptScore W2103042813C86803240 @default.
- W2103042813 hasConceptScore W2103042813C95371953 @default.
- W2103042813 hasIssue "6" @default.
- W2103042813 hasLocation W21030428131 @default.
- W2103042813 hasOpenAccess W2103042813 @default.
- W2103042813 hasPrimaryLocation W21030428131 @default.
- W2103042813 hasRelatedWork W1599915175 @default.
- W2103042813 hasRelatedWork W1974078605 @default.
- W2103042813 hasRelatedWork W1984731778 @default.
- W2103042813 hasRelatedWork W2063775230 @default.
- W2103042813 hasRelatedWork W2073054086 @default.
- W2103042813 hasRelatedWork W2074787602 @default.
- W2103042813 hasRelatedWork W2086814185 @default.