FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103105155> ?p ?o ?g. }

• W2103105155 endingPage "132" @default.
• W2103105155 startingPage "119" @default.
• W2103105155 abstract "The cardiac sarcolemmal Na-Ca exchanger (NCX) is allosterically regulated by [Ca](i) such that when [Ca](i) is low, NCX current (I(NCX)) deactivates. In this study, we used membrane potential (E(m)) and I(NCX) to control Ca entry into and Ca efflux from intact cardiac myocytes to investigate whether this allosteric regulation (Ca activation) occurs with [Ca](i) in the physiological range. In the absence of Ca activation, the electrochemical effect of increasing [Ca](i) would be to increase inward I(NCX) (Ca efflux) and to decrease outward I(NCX). On the other hand, Ca activation would increase I(NCX) in both directions. Thus, we attributed [Ca](i)-dependent increases in outward I(NCX) to allosteric regulation. Ca activation of I(NCX) was observed in ferret myocytes but not in wild-type mouse myocytes, suggesting that Ca regulation of NCX may be species dependent. We also studied transgenic mouse myocytes overexpressing either normal canine NCX or this same canine NCX lacking Ca regulation (Delta680-685). Animals with the normal canine NCX transgene showed Ca activation, whereas animals with the mutant transgene did not, confirming the role of this region in the process. In native ferret cells and in mice with expressed canine NCX, allosteric regulation by Ca occurs under physiological conditions (K(mCaAct) = 125 +/- 16 nM SEM approximately resting [Ca](i)). This, along with the observation that no delay was observed between measured [Ca](i) and activation of I(NCX) under our conditions, suggests that beat to beat changes in NCX function can occur in vivo. These changes in the I(NCX) activation state may influence SR Ca load and resting [Ca](i), helping to fine tune Ca influx and efflux from cells under both normal and pathophysiological conditions. Our failure to observe Ca activation in mouse myocytes may be due to either the extent of Ca regulation or to a difference in K(mCaAct) from other species. Model predictions for Ca activation, on which our estimates of K(mCaAct) are based, confirm that Ca activation strongly influences outward I(NCX), explaining why it increases rather than declines with increasing [Ca](i)." @default.
• W2103105155 created "2016-06-24" @default.
• W2103105155 creator A5020824899 @default.
• W2103105155 creator A5069117498 @default.
• W2103105155 creator A5070247501 @default.
• W2103105155 creator A5074384477 @default.
• W2103105155 creator A5090146824 @default.
• W2103105155 date "2001-01-29" @default.
• W2103105155 modified "2023-09-27" @default.
• W2103105155 title "Allosteric Regulation of Na/Ca Exchange Current by Cytosolic Ca in Intact Cardiac Myocytes" @default.
• W2103105155 cites W1509963147 @default.
• W2103105155 cites W1559767698 @default.
• W2103105155 cites W1857532475 @default.
• W2103105155 cites W1932539454 @default.
• W2103105155 cites W1966880718 @default.
• W2103105155 cites W1974153471 @default.
• W2103105155 cites W1975908160 @default.
• W2103105155 cites W1975945639 @default.
• W2103105155 cites W1980002181 @default.
• W2103105155 cites W1983536345 @default.
• W2103105155 cites W1985578759 @default.
• W2103105155 cites W1996024251 @default.
• W2103105155 cites W1999114304 @default.
• W2103105155 cites W2010213762 @default.
• W2103105155 cites W2017348510 @default.
• W2103105155 cites W2020736572 @default.
• W2103105155 cites W2042926861 @default.
• W2103105155 cites W2050785330 @default.
• W2103105155 cites W2054225000 @default.
• W2103105155 cites W2055484203 @default.
• W2103105155 cites W2059201211 @default.
• W2103105155 cites W2061895140 @default.
• W2103105155 cites W2064351258 @default.
• W2103105155 cites W2065046621 @default.
• W2103105155 cites W2072914802 @default.
• W2103105155 cites W2081941823 @default.
• W2103105155 cites W2089253489 @default.
• W2103105155 cites W2093289575 @default.
• W2103105155 cites W2096795369 @default.
• W2103105155 cites W2097858715 @default.
• W2103105155 cites W2101276997 @default.
• W2103105155 cites W2102924449 @default.
• W2103105155 cites W2119759363 @default.
• W2103105155 cites W2120337309 @default.
• W2103105155 cites W2127585172 @default.
• W2103105155 cites W2132561989 @default.
• W2103105155 cites W2138610459 @default.
• W2103105155 cites W2141260882 @default.
• W2103105155 cites W2147959747 @default.
• W2103105155 cites W2159160674 @default.
• W2103105155 cites W2171218287 @default.
• W2103105155 cites W2219369075 @default.
• W2103105155 cites W2226437153 @default.
• W2103105155 cites W2231651666 @default.
• W2103105155 cites W2408204425 @default.
• W2103105155 cites W2436978206 @default.
• W2103105155 cites W96550900 @default.
• W2103105155 doi "https://doi.org/10.1085/jgp.117.2.119" @default.
• W2103105155 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2217247" @default.
• W2103105155 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11158165" @default.
• W2103105155 hasPublicationYear "2001" @default.
• W2103105155 type Work @default.
• W2103105155 sameAs 2103105155 @default.
• W2103105155 citedByCount "151" @default.
• W2103105155 countsByYear W21031051552012 @default.
• W2103105155 countsByYear W21031051552013 @default.
• W2103105155 countsByYear W21031051552014 @default.
• W2103105155 countsByYear W21031051552015 @default.
• W2103105155 countsByYear W21031051552016 @default.
• W2103105155 countsByYear W21031051552017 @default.
• W2103105155 countsByYear W21031051552018 @default.
• W2103105155 countsByYear W21031051552019 @default.
• W2103105155 countsByYear W21031051552020 @default.
• W2103105155 countsByYear W21031051552021 @default.
• W2103105155 countsByYear W21031051552022 @default.
• W2103105155 countsByYear W21031051552023 @default.
• W2103105155 crossrefType "journal-article" @default.
• W2103105155 hasAuthorship W2103105155A5020824899 @default.
• W2103105155 hasAuthorship W2103105155A5069117498 @default.
• W2103105155 hasAuthorship W2103105155A5070247501 @default.
• W2103105155 hasAuthorship W2103105155A5074384477 @default.
• W2103105155 hasAuthorship W2103105155A5090146824 @default.
• W2103105155 hasBestOaLocation W21031051551 @default.
• W2103105155 hasConcept C12554922 @default.
• W2103105155 hasConcept C126322002 @default.
• W2103105155 hasConcept C134018914 @default.
• W2103105155 hasConcept C166342909 @default.
• W2103105155 hasConcept C178790620 @default.
• W2103105155 hasConcept C181199279 @default.
• W2103105155 hasConcept C181911157 @default.
• W2103105155 hasConcept C185592680 @default.
• W2103105155 hasConcept C207200792 @default.
• W2103105155 hasConcept C2777735026 @default.
• W2103105155 hasConcept C519063684 @default.
• W2103105155 hasConcept C55493867 @default.
• W2103105155 hasConcept C63645605 @default.
• W2103105155 hasConcept C71924100 @default.
• W2103105155 hasConcept C79879829 @default.

• next