Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103105540> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2103105540 abstract "Recently vibratome-cut heart slices has been proposed as a new model in cardiac research, particularly in studying functional integration of transplanted donor stem cells into the host myocardium. This emerging model has, over co-culture models, a theoretical advantage in maintaining the 3-dimensional cardiac structure and physiological cell-cell connections; however, further validation is needed for this to be an established model. Particularly, the ability of cardiomyocytes in heart slices to connect with administered donor cells remains uncertain. Heart slices of 300 μm thickness were cut from adult and embryonic rat hearts using a vibratome and cultured. Adult heart slices maintained localized contraction for up to 3 days. Various histological studies demonstrated that the myocardial morphology was well preserved with necrotic cells being restricted to the edges of slices, but the number of apoptotic cells in the slices increased with culture periods (16.0 ± 3.2% at day 2 and 59.1 ± 8.3% at day 6). Importantly, immunohistolabeling and western blotting showed that connexin43 expression, which is a prerequisite for gap junction formation between donor-derived and host cardiomyocytes, was markedly diminished by 1 day in culture, suggesting rapid functional deterioration of adult heart slices. In contrast, embryonic heart slices remained contracting at least for 35 days with myocardial morphology and connexin43-involved gap junctions remaining intact, though in the embryonic, immature pattern. Collectively, both adult and embryonic rodent heart slices, in the current style, have critical limitations to assess integration between donor-derived and host cardiomyocytes. Further study to improve the quality of heart slices is warranted." @default.
- W2103105540 created "2016-06-24" @default.
- W2103105540 creator A5006626017 @default.
- W2103105540 creator A5011158638 @default.
- W2103105540 creator A5029347863 @default.
- W2103105540 creator A5042851375 @default.
- W2103105540 creator A5062288838 @default.
- W2103105540 date "2012-01-01" @default.
- W2103105540 modified "2023-09-25" @default.
- W2103105540 title "Histological Validation of Heart Slices as a Model in Cardiac Research" @default.
- W2103105540 cites W1483809387 @default.
- W2103105540 cites W1574235604 @default.
- W2103105540 cites W160771735 @default.
- W2103105540 cites W1862736921 @default.
- W2103105540 cites W190126928 @default.
- W2103105540 cites W1965814444 @default.
- W2103105540 cites W1976309047 @default.
- W2103105540 cites W1986275371 @default.
- W2103105540 cites W1997909474 @default.
- W2103105540 cites W2001535568 @default.
- W2103105540 cites W2014744211 @default.
- W2103105540 cites W2016931107 @default.
- W2103105540 cites W2019695327 @default.
- W2103105540 cites W2025971542 @default.
- W2103105540 cites W2039564450 @default.
- W2103105540 cites W2061413411 @default.
- W2103105540 cites W2062854762 @default.
- W2103105540 cites W2064930644 @default.
- W2103105540 cites W2065837488 @default.
- W2103105540 cites W2083077654 @default.
- W2103105540 cites W2084109090 @default.
- W2103105540 cites W2084889822 @default.
- W2103105540 cites W2085429963 @default.
- W2103105540 cites W2100458582 @default.
- W2103105540 cites W2114135413 @default.
- W2103105540 cites W2122106267 @default.
- W2103105540 cites W2124573362 @default.
- W2103105540 cites W2130668754 @default.
- W2103105540 cites W2131279054 @default.
- W2103105540 cites W2135358140 @default.
- W2103105540 cites W2135791659 @default.
- W2103105540 cites W2149195526 @default.
- W2103105540 cites W2149455838 @default.
- W2103105540 cites W2152533263 @default.
- W2103105540 doi "https://doi.org/10.4172/2157-7013.1000126" @default.
- W2103105540 hasPublicationYear "2012" @default.
- W2103105540 type Work @default.
- W2103105540 sameAs 2103105540 @default.
- W2103105540 citedByCount "5" @default.
- W2103105540 countsByYear W21031055402014 @default.
- W2103105540 countsByYear W21031055402015 @default.
- W2103105540 countsByYear W21031055402016 @default.
- W2103105540 countsByYear W21031055402017 @default.
- W2103105540 crossrefType "journal-article" @default.
- W2103105540 hasAuthorship W2103105540A5006626017 @default.
- W2103105540 hasAuthorship W2103105540A5011158638 @default.
- W2103105540 hasAuthorship W2103105540A5029347863 @default.
- W2103105540 hasAuthorship W2103105540A5042851375 @default.
- W2103105540 hasAuthorship W2103105540A5062288838 @default.
- W2103105540 hasConcept C126322002 @default.
- W2103105540 hasConcept C136229726 @default.
- W2103105540 hasConcept C164705383 @default.
- W2103105540 hasConcept C185592680 @default.
- W2103105540 hasConcept C41008148 @default.
- W2103105540 hasConcept C71924100 @default.
- W2103105540 hasConceptScore W2103105540C126322002 @default.
- W2103105540 hasConceptScore W2103105540C136229726 @default.
- W2103105540 hasConceptScore W2103105540C164705383 @default.
- W2103105540 hasConceptScore W2103105540C185592680 @default.
- W2103105540 hasConceptScore W2103105540C41008148 @default.
- W2103105540 hasConceptScore W2103105540C71924100 @default.
- W2103105540 hasIssue "04" @default.
- W2103105540 hasLocation W21031055401 @default.
- W2103105540 hasOpenAccess W2103105540 @default.
- W2103105540 hasPrimaryLocation W21031055401 @default.
- W2103105540 hasRelatedWork W1531601525 @default.
- W2103105540 hasRelatedWork W2748952813 @default.
- W2103105540 hasRelatedWork W2758277628 @default.
- W2103105540 hasRelatedWork W2899084033 @default.
- W2103105540 hasRelatedWork W2935909890 @default.
- W2103105540 hasRelatedWork W2948807893 @default.
- W2103105540 hasRelatedWork W3173606202 @default.
- W2103105540 hasRelatedWork W3183948672 @default.
- W2103105540 hasRelatedWork W2778153218 @default.
- W2103105540 hasRelatedWork W3110381201 @default.
- W2103105540 hasVolume "03" @default.
- W2103105540 isParatext "false" @default.
- W2103105540 isRetracted "false" @default.
- W2103105540 magId "2103105540" @default.
- W2103105540 workType "article" @default.