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• W2103131130 abstract "Abstract Background There are many potential sources of variability in a microarray experiment. Variation can arise from many aspects of the collection and processing of samples for gene expression analysis. Oligonucleotide-based arrays are thought to minimize one source of variability as identical oligonucleotides are expected to recognize the same transcripts during hybridization. Results We demonstrate that although the probes on the U133A GeneChip arrays are identical in sequence to probes designed for the U133 Plus 2.0 arrays the values obtained from an experimental hybridization can be quite different. Nearly half of the probesets in common between the two array types can produce slightly different values from the same sample. Nearly 70% of the individual probes in these probesets produced array specific differences. Conclusion The context of the probe may also contribute some bias to the final measured value of gene expression. At a minimum, this should add an extra level of caution when considering the direct comparison of experiments performed in two microarray formats. More importantly, this suggests that it may not be possible to know which value is the most accurate representation of a biological sample when comparing two formats." @default.
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• W2103131130 date "2006-06-15" @default.
• W2103131130 modified "2023-10-09" @default.
• W2103131130 title "Identical probes on different high-density oligonucleotide microarrays can produce different measurements of gene expression" @default.
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• W2103131130 doi "https://doi.org/10.1186/1471-2164-7-153" @default.
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