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- W2103139056 abstract "Summary Synchrotron radiation computed laminography ( SR ‐ CL ) is presented as an imaging method for analyzing the three‐dimensional (3 D ) anatomy of leaves. The SR ‐ CL method was used to provide 3 D images of 1‐mm² samples of intact leaves at a pixel resolution of 750 nm. The method allowed visualization and quantitative analysis of palisade and spongy mesophyll cells, and showed local venation patterns, aspects of xylem vascular structure and stomata. The method failed to image subcellular organelles such as chloroplasts. We constructed 3 D computer models of leaves that can provide a basis for calculating gas exchange, light penetration and water and solute transport. The leaf anatomy of two different tomato genotypes grown in saturating light conditions was compared by 3 D analysis. Differences were found in calculated values of tissue porosity, cell number density, cell area to volume ratio and cell volume and cell shape distributions of palisade and spongy cell layers. In contrast, the exposed cell area to leaf area ratio in mesophyll, a descriptor that correlates to the maximum rate of photosynthesis in saturated light conditions, was no different between spongy and palisade cells or between genotypes. The use of 3 D image processing avoids many of the limitations of anatomical analysis with two‐dimensional sections." @default.
- W2103139056 created "2016-06-24" @default.
- W2103139056 creator A5000573174 @default.
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- W2103139056 date "2014-11-15" @default.
- W2103139056 modified "2023-10-03" @default.
- W2103139056 title "Synchrotron X-ray computed laminography of the three-dimensional anatomy of tomato leaves" @default.
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