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• W2103141817 abstract "In Paracoccidioides brasiliensis, a dimorphic fungus pathogenic for humans, no significant differences were observed in the phospholipid species of both morphological phases. The species observed were phosphatidylcholine (PC, 30–40%), phosphatidylethanolamine (PE, 27-28%), phosphatidylserine (16–19%), phosphatidylinositol (13–17%) and sphingomyelin (3–5–0025;). The main fatty acids found in the yeast (Y) phase were palmitate (56%), linoleate (18%) and oleate (15%), while linoleate predominated (61 %) in the mycelial (M) phase, followed by palmitate (27%) and oleate (7%). In the Y phase the main free sterol was ergosta-5,22-dien-3β-ol (82%) plus some lanosterol (12%) and ergosterol (6%), while in the M phase, the latter predominated (88%), followed by low levels of ergosta-5,22-dien-3β-ol (12%). Ajoene [( E,Z )-4,5,9-trithiadodeca-1,6,11-triene 9-oxide], a platelet aggregation inhibitor derived from garlic, induced alterations in phospholipid and fatty acid proportions such that PC was reduced to about 18% in both phases and PE increased to 38% (Y phase) or 44% (M phase), suggesting inhibition of PC synthesis. Ajoene also reduced saturated fatty acids (16:0 and 18:0) from 67 to 35% in the Y phase, with a corresponding increase in the unsaturated components. This effect was not seen in the M phase." @default.
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• W2103141817 date "1997-05-01" @default.
• W2103141817 modified "2023-10-01" @default.
• W2103141817 title "Paracoccidioides brasiliensis by ajoene is associated with blockade of phosphatidylcholine biosynthesis" @default.
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• W2103141817 doi "https://doi.org/10.1099/00221287-143-5-1583" @default.
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