FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103149871> ?p ?o ?g. }

• W2103149871 endingPage "211" @default.
• W2103149871 startingPage "205" @default.
• W2103149871 abstract "To achive the coarse purification of a monoclonal antibody from whole hybridoma fermentation broth a fluidized bed cation exchange process was used. The procedure consisted of application of the crude sample and washing of the bed in a fluidized mode and elution in a fixed bed mode. A completely clarified eluate was obtained with purification factors between 4 and 8 and a concentration of the desired product (monoclonal antibody) by a factor of more than 3 was achived. Thus, a combination of the three early steps of the downstream process clarification, concentration and coarse purification was possible. Two different materials were tested: a commercially available agarose-based matrix (Stream-line-SP), and a self-derivatized material based on controlled-pore glass (Bioran). Initial experiments were performed to describe the fluidization of the glass material. Comparison with the agarose material showed several differences, the agarose matrix allowing liquid flow closer to plug flow than the glass material. Increased backmixing in the liquid phase was detected when fluidizing the glass adsorbent compared with the agarose-based matrix. Despite this fact, comparison of the two materials with respect to antibody binding and elution demonstrated a similar performance. © 1995 John Wiley & Sons, Inc." @default.
• W2103149871 created "2016-06-24" @default.
• W2103149871 creator A5007473683 @default.
• W2103149871 creator A5014058116 @default.
• W2103149871 creator A5048774095 @default.
• W2103149871 creator A5065769561 @default.
• W2103149871 date "1995-02-05" @default.
• W2103149871 modified "2023-09-23" @default.
• W2103149871 title "Purification of monoclonal antibodies from whole hybridoma fermentation broth by fluidized bed adsorption" @default.
• W2103149871 cites W1550336457 @default.
• W2103149871 cites W1608481638 @default.
• W2103149871 cites W170752630 @default.
• W2103149871 cites W196796496 @default.
• W2103149871 cites W1971831173 @default.
• W2103149871 cites W1976376655 @default.
• W2103149871 cites W1980687301 @default.
• W2103149871 cites W2008401153 @default.
• W2103149871 cites W2017727552 @default.
• W2103149871 cites W2019500063 @default.
• W2103149871 cites W2036417053 @default.
• W2103149871 cites W2061005588 @default.
• W2103149871 cites W2061670429 @default.
• W2103149871 cites W2062453538 @default.
• W2103149871 cites W2074581042 @default.
• W2103149871 cites W2082146236 @default.
• W2103149871 cites W2082495558 @default.
• W2103149871 cites W2157173693 @default.
• W2103149871 cites W795974976 @default.
• W2103149871 doi "https://doi.org/10.1002/bit.260450304" @default.
• W2103149871 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18623139" @default.
• W2103149871 hasPublicationYear "1995" @default.
• W2103149871 type Work @default.
• W2103149871 sameAs 2103149871 @default.
• W2103149871 citedByCount "86" @default.
• W2103149871 countsByYear W21031498712014 @default.
• W2103149871 countsByYear W21031498712015 @default.
• W2103149871 countsByYear W21031498712017 @default.
• W2103149871 countsByYear W21031498712018 @default.
• W2103149871 countsByYear W21031498712022 @default.
• W2103149871 crossrefType "journal-article" @default.
• W2103149871 hasAuthorship W2103149871A5007473683 @default.
• W2103149871 hasAuthorship W2103149871A5014058116 @default.
• W2103149871 hasAuthorship W2103149871A5048774095 @default.
• W2103149871 hasAuthorship W2103149871A5065769561 @default.
• W2103149871 hasConcept C100544194 @default.
• W2103149871 hasConcept C106487976 @default.
• W2103149871 hasConcept C150394285 @default.
• W2103149871 hasConcept C159654299 @default.
• W2103149871 hasConcept C168170006 @default.
• W2103149871 hasConcept C178790620 @default.
• W2103149871 hasConcept C185592680 @default.
• W2103149871 hasConcept C194832619 @default.
• W2103149871 hasConcept C203014093 @default.
• W2103149871 hasConcept C207891110 @default.
• W2103149871 hasConcept C2084832 @default.
• W2103149871 hasConcept C2778476535 @default.
• W2103149871 hasConcept C43617362 @default.
• W2103149871 hasConcept C542903549 @default.
• W2103149871 hasConcept C55493867 @default.
• W2103149871 hasConcept C67568554 @default.
• W2103149871 hasConcept C73826308 @default.
• W2103149871 hasConcept C86803240 @default.
• W2103149871 hasConceptScore W2103149871C100544194 @default.
• W2103149871 hasConceptScore W2103149871C106487976 @default.
• W2103149871 hasConceptScore W2103149871C150394285 @default.
• W2103149871 hasConceptScore W2103149871C159654299 @default.
• W2103149871 hasConceptScore W2103149871C168170006 @default.
• W2103149871 hasConceptScore W2103149871C178790620 @default.
• W2103149871 hasConceptScore W2103149871C185592680 @default.
• W2103149871 hasConceptScore W2103149871C194832619 @default.
• W2103149871 hasConceptScore W2103149871C203014093 @default.
• W2103149871 hasConceptScore W2103149871C207891110 @default.
• W2103149871 hasConceptScore W2103149871C2084832 @default.
• W2103149871 hasConceptScore W2103149871C2778476535 @default.
• W2103149871 hasConceptScore W2103149871C43617362 @default.
• W2103149871 hasConceptScore W2103149871C542903549 @default.
• W2103149871 hasConceptScore W2103149871C55493867 @default.
• W2103149871 hasConceptScore W2103149871C67568554 @default.
• W2103149871 hasConceptScore W2103149871C73826308 @default.
• W2103149871 hasConceptScore W2103149871C86803240 @default.
• W2103149871 hasIssue "3" @default.
• W2103149871 hasLocation W21031498711 @default.
• W2103149871 hasLocation W21031498712 @default.
• W2103149871 hasOpenAccess W2103149871 @default.
• W2103149871 hasPrimaryLocation W21031498711 @default.
• W2103149871 hasRelatedWork W1587569393 @default.
• W2103149871 hasRelatedWork W1991047725 @default.
• W2103149871 hasRelatedWork W2014093098 @default.
• W2103149871 hasRelatedWork W2053625711 @default.
• W2103149871 hasRelatedWork W2068352812 @default.
• W2103149871 hasRelatedWork W2072683930 @default.
• W2103149871 hasRelatedWork W2103149871 @default.
• W2103149871 hasRelatedWork W2392705931 @default.
• W2103149871 hasRelatedWork W3214254418 @default.
• W2103149871 hasRelatedWork W4250075450 @default.
• W2103149871 hasVolume "45" @default.
• W2103149871 isParatext "false" @default.
• W2103149871 isRetracted "false" @default.

• next