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- W2103151097 abstract "Development of cholesterol biosensors is of great importance in clinical analysis because the concentration ofcholesterol in blood is a fundamental parameter for the prevention and diagnosis of a number of clinical disorders suchas heart disease, hypertension and arteriosclerosis. In general, determination of cholesterol is based onspectrophotometry; but this method involves complicated procedures and the cost is high because expensive enzymemust be used in each assay.We report here the observation, for the first time, of the enhancement of Europium-Tetracycline complexemission in cholesterol solutions. This enhancement was initially observed with the addition of the enzyme cholesteroloxidase, which produces H 2 O 2 , the agent driver of the Europium tetracycline complex, to the solution. However, it wasfound that the enzyme is not needed to enhance the luminescence. A calibration curve was determined, resulting in aneasy-handling immobilization method with a cheap stable material. This method shows that the complex can be used as asensor to determine cholesterol in biological systems with good selectivity, fast response, miniature size, andreproducible results." @default.
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- W2103151097 date "2007-02-08" @default.
- W2103151097 modified "2023-10-03" @default.
- W2103151097 title "Europium tetracycline biosensor for the determination of cholesterol" @default.
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- W2103151097 doi "https://doi.org/10.1117/12.697956" @default.
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