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• W2103161436 abstract "Pericytes surround capillary endothelial cells and exert contractile forces modulating microvascular tone and endothelial growth. We previously described pericyte contractile phenotype to be Rho GTPase- and α-smooth muscle actin (αSMA)-dependent. However, mechanisms mediating adhesion-dependent shape changes and contractile force transduction remain largely equivocal. We now report that the neutral cysteine protease, calpain, modulates pericyte contractility and cellular stiffness via talin, an integrin-binding and F-actin associating protein. Digital imaging and quantitative analyses of living cells reveal significant perturbations in contractile force transduction detected via deformation of silicone substrata, as well as perturbations of mechanical stiffness in cellular contractile subdomains quantified via atomic force microscope (AFM)-enabled nanoindentation. Pericytes overexpressing GFP-tagged talin show significantly enhanced contractility (~ two-fold), which is mitigated when either the calpain-cleavage resistant mutant talin L432G or vinculin are expressed. Moreover, the cell-penetrating, calpain-specific inhibitor termed CALPASTAT reverses talin-enhanced, but not Rho GTP-dependent, contractility. Interestingly, our analysis revealed that CALPASTAT, but not its inactive mutant, alters contractile cell-driven substrata deformations while increasing mechanical stiffness of subcellular contractile regions of these pericytes. Altogether, our results reveal that calpain-dependent cleavage of talin modulates cell contractile dynamics, which in pericytes may prove instrumental in controlling normal capillary function or microvascular pathophysiology." @default.
• W2103161436 created "2016-06-24" @default.
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• W2103161436 creator A5077822196 @default.
• W2103161436 date "2010-12-01" @default.
• W2103161436 modified "2023-10-18" @default.
• W2103161436 title "Calpain- and talin-dependent control of microvascular pericyte contractility and cellular stiffness" @default.
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• W2103161436 doi "https://doi.org/10.1016/j.mvr.2010.07.012" @default.
• W2103161436 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2981705" @default.
• W2103161436 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20709086" @default.
• W2103161436 hasPublicationYear "2010" @default.
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