Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103164029> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2103164029 endingPage "773" @default.
- W2103164029 startingPage "770" @default.
- W2103164029 abstract "BACKGROUND The presence of the EGFR (epidermal growth factor receptor) T790M mutation in tumor tissue or body fluids from patients treated with EGFR tyrosine kinase inhibitors may indicate the onset of resistance to treatment. It is important to identify this mutation as early as possible so that treatment can be modified accordingly or potential side effects of further treatment can be avoided. This requirement calls for high detection sensitivity. Peptide nucleic acids (PNAs) are used as PCR clamps to inhibit amplification of wild-type DNA during PCR cycling, thereby enriching for rare mutations such as T790M. We describe a modification that improves the detection limit of PNA-clamp methods by at least 20-fold. METHODS We enriched the target by exposing genomic DNA to an EGFR exon 20–specific biotinylated oligonucleotide, followed by binding to streptavidin beads. We then prepared serial dilutions of the isolated target DNA containing the T790M mutation by mixing with wild-type DNA and then performed PNA clamp–based, real-time TaqMan PCR. For comparison, we performed PNA clamp–based PCR directly on genomic DNA. RESULTS Whereas the detection limit for PNA clamp–based PCR performed directly on genomic DNA is 1 mutant allele in 1000 wild-type alleles, conducting the assay with biotinylated oligonucleotide–enriched target DNA improved the detection limit to 1 mutant allele in 40 000 wild-type alleles. A possible explanation for the improvement in detection is that biotin-based target isolation efficiently eliminates wild-type DNA; therefore, fewer erroneous amplifications of wild-type DNA can occur early during the PCR. CONCLUSIONS Combining target molecule isolation via a biotinylated probe with PNA-enriched TaqMan real-time PCR provides a major improvement for detecting the EGFR T790M resistance mutation." @default.
- W2103164029 created "2016-06-24" @default.
- W2103164029 creator A5029636776 @default.
- W2103164029 creator A5048675362 @default.
- W2103164029 creator A5058353577 @default.
- W2103164029 date "2011-05-01" @default.
- W2103164029 modified "2023-10-17" @default.
- W2103164029 title "Biotinylated Probe Isolation of Targeted Gene Region Improves Detection of T790M Epidermal Growth Factor Receptor Mutation via Peptide Nucleic Acid–Enriched Real-Time PCR" @default.
- W2103164029 cites W1907370866 @default.
- W2103164029 cites W2002937040 @default.
- W2103164029 cites W2003984225 @default.
- W2103164029 cites W2015000811 @default.
- W2103164029 cites W2021312892 @default.
- W2103164029 cites W2061927660 @default.
- W2103164029 cites W2063728375 @default.
- W2103164029 cites W2068244265 @default.
- W2103164029 cites W2068262084 @default.
- W2103164029 cites W2071251869 @default.
- W2103164029 cites W2103803613 @default.
- W2103164029 cites W2104669758 @default.
- W2103164029 cites W2113647632 @default.
- W2103164029 cites W2119167595 @default.
- W2103164029 cites W2126455294 @default.
- W2103164029 cites W2129736970 @default.
- W2103164029 cites W2135125731 @default.
- W2103164029 cites W2136263263 @default.
- W2103164029 cites W2139799027 @default.
- W2103164029 cites W2140334822 @default.
- W2103164029 cites W2159322582 @default.
- W2103164029 doi "https://doi.org/10.1373/clinchem.2010.157784" @default.
- W2103164029 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3208327" @default.
- W2103164029 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21325655" @default.
- W2103164029 hasPublicationYear "2011" @default.
- W2103164029 type Work @default.
- W2103164029 sameAs 2103164029 @default.
- W2103164029 citedByCount "13" @default.
- W2103164029 countsByYear W21031640292012 @default.
- W2103164029 countsByYear W21031640292013 @default.
- W2103164029 countsByYear W21031640292015 @default.
- W2103164029 countsByYear W21031640292016 @default.
- W2103164029 countsByYear W21031640292017 @default.
- W2103164029 countsByYear W21031640292018 @default.
- W2103164029 countsByYear W21031640292019 @default.
- W2103164029 countsByYear W21031640292022 @default.
- W2103164029 crossrefType "journal-article" @default.
- W2103164029 hasAuthorship W2103164029A5029636776 @default.
- W2103164029 hasAuthorship W2103164029A5048675362 @default.
- W2103164029 hasAuthorship W2103164029A5058353577 @default.
- W2103164029 hasBestOaLocation W21031640291 @default.
- W2103164029 hasConcept C104317684 @default.
- W2103164029 hasConcept C129312508 @default.
- W2103164029 hasConcept C143065580 @default.
- W2103164029 hasConcept C14363569 @default.
- W2103164029 hasConcept C153911025 @default.
- W2103164029 hasConcept C17757408 @default.
- W2103164029 hasConcept C204628709 @default.
- W2103164029 hasConcept C24107716 @default.
- W2103164029 hasConcept C2756471 @default.
- W2103164029 hasConcept C2777343594 @default.
- W2103164029 hasConcept C552990157 @default.
- W2103164029 hasConcept C55493867 @default.
- W2103164029 hasConcept C86803240 @default.
- W2103164029 hasConceptScore W2103164029C104317684 @default.
- W2103164029 hasConceptScore W2103164029C129312508 @default.
- W2103164029 hasConceptScore W2103164029C143065580 @default.
- W2103164029 hasConceptScore W2103164029C14363569 @default.
- W2103164029 hasConceptScore W2103164029C153911025 @default.
- W2103164029 hasConceptScore W2103164029C17757408 @default.
- W2103164029 hasConceptScore W2103164029C204628709 @default.
- W2103164029 hasConceptScore W2103164029C24107716 @default.
- W2103164029 hasConceptScore W2103164029C2756471 @default.
- W2103164029 hasConceptScore W2103164029C2777343594 @default.
- W2103164029 hasConceptScore W2103164029C552990157 @default.
- W2103164029 hasConceptScore W2103164029C55493867 @default.
- W2103164029 hasConceptScore W2103164029C86803240 @default.
- W2103164029 hasFunder F4320332161 @default.
- W2103164029 hasIssue "5" @default.
- W2103164029 hasLocation W21031640291 @default.
- W2103164029 hasLocation W21031640292 @default.
- W2103164029 hasLocation W21031640293 @default.
- W2103164029 hasLocation W21031640294 @default.
- W2103164029 hasOpenAccess W2103164029 @default.
- W2103164029 hasPrimaryLocation W21031640291 @default.
- W2103164029 hasRelatedWork W1593893811 @default.
- W2103164029 hasRelatedWork W1970008822 @default.
- W2103164029 hasRelatedWork W1973529384 @default.
- W2103164029 hasRelatedWork W2067763881 @default.
- W2103164029 hasRelatedWork W2086743682 @default.
- W2103164029 hasRelatedWork W2130310904 @default.
- W2103164029 hasRelatedWork W2222711268 @default.
- W2103164029 hasRelatedWork W2555696200 @default.
- W2103164029 hasRelatedWork W4250325211 @default.
- W2103164029 hasRelatedWork W2153269459 @default.
- W2103164029 hasVolume "57" @default.
- W2103164029 isParatext "false" @default.
- W2103164029 isRetracted "false" @default.
- W2103164029 magId "2103164029" @default.
- W2103164029 workType "article" @default.