Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103171057> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2103171057 endingPage "4766" @default.
- W2103171057 startingPage "4762" @default.
- W2103171057 abstract "ABSTRACT Mycoplasma hyopneumoniae colonizes the swine respiratory tract at the level of ciliated cells by attaching specifically to the cilium membrane. This interaction involves an adhesin called P97; the cilium binding activity of this protein was localized to the carboxy terminus, which included two repeat regions, R1 and R2 (T. Hsu, S. Artiushin, and F. C. Minion, J. Bacteriol. 179:1317–1323, 1997). To further delineate the molecular mechanisms of M. hyopneumoniae interactions with ciliated epithelium, we used a bank of transposon inserts in the cloned P97 gene to identify the site for cilium binding by testing the truncated gene products in an in vitro microtiter plate adherence assay. These studies showed that the cilium binding site was located in the AAKPV(E) repeat sequence of P97, referred to as the R1 repeat. For functional binding, at least seven AAKPV(E) repeats were required. The adherence-blocking monoclonal antibody F1B6 also recognized this region but required fewer AAKPV(E) repeats for recognition. We then constructed R1 region- lacZ gene fusions and used the resulting R1 repeat–β-galactosidase fusion proteins in an in vitro assay to confirm the role of R1 in cilium binding. A comparison of the R1 regions of M. hyopneumoniae strains displaying variation in cilium adherence failed to identify changes that could account for the differences in adherence shown by the strains. Thus, we concluded that other proteins, in addition to P97, must be involved in cilium adherence, possibly in combination with P97." @default.
- W2103171057 created "2016-06-24" @default.
- W2103171057 creator A5014987110 @default.
- W2103171057 creator A5031503507 @default.
- W2103171057 date "1998-10-01" @default.
- W2103171057 modified "2023-10-12" @default.
- W2103171057 title "Identification of the Cilium Binding Epitope of the <i>Mycoplasma hyopneumoniae</i> P97 Adhesin" @default.
- W2103171057 cites W1915279081 @default.
- W2103171057 cites W1991710323 @default.
- W2103171057 cites W2004826215 @default.
- W2103171057 cites W2018289835 @default.
- W2103171057 cites W2039393616 @default.
- W2103171057 cites W2075240542 @default.
- W2103171057 cites W2101108802 @default.
- W2103171057 cites W2101597645 @default.
- W2103171057 cites W2124283227 @default.
- W2103171057 cites W2132417094 @default.
- W2103171057 cites W2132941452 @default.
- W2103171057 cites W2159497906 @default.
- W2103171057 cites W2171059292 @default.
- W2103171057 cites W2171880541 @default.
- W2103171057 cites W2408207024 @default.
- W2103171057 cites W4255060601 @default.
- W2103171057 cites W47192821 @default.
- W2103171057 doi "https://doi.org/10.1128/iai.66.10.4762-4766.1998" @default.
- W2103171057 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/108587" @default.
- W2103171057 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9746576" @default.
- W2103171057 hasPublicationYear "1998" @default.
- W2103171057 type Work @default.
- W2103171057 sameAs 2103171057 @default.
- W2103171057 citedByCount "88" @default.
- W2103171057 countsByYear W21031710572012 @default.
- W2103171057 countsByYear W21031710572013 @default.
- W2103171057 countsByYear W21031710572014 @default.
- W2103171057 countsByYear W21031710572016 @default.
- W2103171057 countsByYear W21031710572017 @default.
- W2103171057 countsByYear W21031710572018 @default.
- W2103171057 countsByYear W21031710572019 @default.
- W2103171057 countsByYear W21031710572020 @default.
- W2103171057 countsByYear W21031710572021 @default.
- W2103171057 countsByYear W21031710572022 @default.
- W2103171057 countsByYear W21031710572023 @default.
- W2103171057 crossrefType "journal-article" @default.
- W2103171057 hasAuthorship W2103171057A5014987110 @default.
- W2103171057 hasAuthorship W2103171057A5031503507 @default.
- W2103171057 hasBestOaLocation W21031710571 @default.
- W2103171057 hasConcept C100094513 @default.
- W2103171057 hasConcept C104317684 @default.
- W2103171057 hasConcept C115811362 @default.
- W2103171057 hasConcept C153911025 @default.
- W2103171057 hasConcept C159654299 @default.
- W2103171057 hasConcept C179470777 @default.
- W2103171057 hasConcept C195616568 @default.
- W2103171057 hasConcept C24870808 @default.
- W2103171057 hasConcept C2780383596 @default.
- W2103171057 hasConcept C54355233 @default.
- W2103171057 hasConcept C60987743 @default.
- W2103171057 hasConcept C86803240 @default.
- W2103171057 hasConcept C95444343 @default.
- W2103171057 hasConceptScore W2103171057C100094513 @default.
- W2103171057 hasConceptScore W2103171057C104317684 @default.
- W2103171057 hasConceptScore W2103171057C115811362 @default.
- W2103171057 hasConceptScore W2103171057C153911025 @default.
- W2103171057 hasConceptScore W2103171057C159654299 @default.
- W2103171057 hasConceptScore W2103171057C179470777 @default.
- W2103171057 hasConceptScore W2103171057C195616568 @default.
- W2103171057 hasConceptScore W2103171057C24870808 @default.
- W2103171057 hasConceptScore W2103171057C2780383596 @default.
- W2103171057 hasConceptScore W2103171057C54355233 @default.
- W2103171057 hasConceptScore W2103171057C60987743 @default.
- W2103171057 hasConceptScore W2103171057C86803240 @default.
- W2103171057 hasConceptScore W2103171057C95444343 @default.
- W2103171057 hasIssue "10" @default.
- W2103171057 hasLocation W21031710571 @default.
- W2103171057 hasLocation W21031710572 @default.
- W2103171057 hasLocation W21031710573 @default.
- W2103171057 hasLocation W21031710574 @default.
- W2103171057 hasOpenAccess W2103171057 @default.
- W2103171057 hasPrimaryLocation W21031710571 @default.
- W2103171057 hasRelatedWork W1972359298 @default.
- W2103171057 hasRelatedWork W2004246922 @default.
- W2103171057 hasRelatedWork W2103171057 @default.
- W2103171057 hasRelatedWork W2157367253 @default.
- W2103171057 hasRelatedWork W2158440121 @default.
- W2103171057 hasRelatedWork W2606833132 @default.
- W2103171057 hasRelatedWork W2729680997 @default.
- W2103171057 hasRelatedWork W3020439838 @default.
- W2103171057 hasRelatedWork W3044737290 @default.
- W2103171057 hasRelatedWork W4288051858 @default.
- W2103171057 hasVolume "66" @default.
- W2103171057 isParatext "false" @default.
- W2103171057 isRetracted "false" @default.
- W2103171057 magId "2103171057" @default.
- W2103171057 workType "article" @default.