FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103172172> ?p ?o ?g. }

• W2103172172 endingPage "57" @default.
• W2103172172 startingPage "53" @default.
• W2103172172 abstract "Amyloid-β peptide 1–42 (Aβ1–42), the predominant form in senile plaques, plays important roles in the pathogenesis of Alzheimer’s disease. Because Aβ1–42 has aggregation-prone nature, it has been difficult to produce in a soluble state in bacterial expression systems. In this study, we modified our expression system to increase the soluble fraction of Aβ1–42 in Escherichia coli (E. coli) cells. The expression level and solubility of recombinant Aβ1–42 induced at the low temperature (16 °C) is highly increased compared to that induced at 37 °C. To optimize expression temperature, the coding region of Aβ1–42 was constructed in a pCold vector, pCold–TF, which has a hexahistidine-tagged trigger factor (TF). Recombinant Aβ1–42 was expressed primarily as a soluble protein using pCold vector system and purified with a nickel-chelating resin. When the toxic effect of recombinant Aβ1–42 examined on human neuroblastoma SH-SY5Y cells, the purified Aβ1–42 induced cell toxicity on SH-SY5Y cells. In conclusion, the system developed in this study will provide a useful method for the production of aggregation prone-peptide such as Aβ1–42." @default.
• W2103172172 created "2016-06-24" @default.
• W2103172172 creator A5008324988 @default.
• W2103172172 creator A5020445056 @default.
• W2103172172 creator A5027381296 @default.
• W2103172172 creator A5043801433 @default.
• W2103172172 creator A5050433869 @default.
• W2103172172 creator A5051490033 @default.
• W2103172172 creator A5058372327 @default.
• W2103172172 creator A5063083094 @default.
• W2103172172 date "2012-11-01" @default.
• W2103172172 modified "2023-10-01" @default.
• W2103172172 title "Large-scale production of soluble recombinant amyloid-β peptide 1–42 using cold-inducible expression system" @default.
• W2103172172 cites W1532542691 @default.
• W2103172172 cites W1677883125 @default.
• W2103172172 cites W1964927015 @default.
• W2103172172 cites W1969165181 @default.
• W2103172172 cites W1973479349 @default.
• W2103172172 cites W1981242112 @default.
• W2103172172 cites W1981570625 @default.
• W2103172172 cites W1981685694 @default.
• W2103172172 cites W1986060177 @default.
• W2103172172 cites W1989948422 @default.
• W2103172172 cites W2031958434 @default.
• W2103172172 cites W2036288989 @default.
• W2103172172 cites W2076845200 @default.
• W2103172172 cites W2094411486 @default.
• W2103172172 cites W2117500006 @default.
• W2103172172 cites W2128451732 @default.
• W2103172172 cites W2130111915 @default.
• W2103172172 doi "https://doi.org/10.1016/j.pep.2012.08.021" @default.
• W2103172172 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22982229" @default.
• W2103172172 hasPublicationYear "2012" @default.
• W2103172172 type Work @default.
• W2103172172 sameAs 2103172172 @default.
• W2103172172 citedByCount "19" @default.
• W2103172172 countsByYear W21031721722012 @default.
• W2103172172 countsByYear W21031721722013 @default.
• W2103172172 countsByYear W21031721722014 @default.
• W2103172172 countsByYear W21031721722015 @default.
• W2103172172 countsByYear W21031721722016 @default.
• W2103172172 countsByYear W21031721722018 @default.
• W2103172172 countsByYear W21031721722019 @default.
• W2103172172 countsByYear W21031721722020 @default.
• W2103172172 countsByYear W21031721722021 @default.
• W2103172172 countsByYear W21031721722022 @default.
• W2103172172 countsByYear W21031721722023 @default.
• W2103172172 crossrefType "journal-article" @default.
• W2103172172 hasAuthorship W2103172172A5008324988 @default.
• W2103172172 hasAuthorship W2103172172A5020445056 @default.
• W2103172172 hasAuthorship W2103172172A5027381296 @default.
• W2103172172 hasAuthorship W2103172172A5043801433 @default.
• W2103172172 hasAuthorship W2103172172A5050433869 @default.
• W2103172172 hasAuthorship W2103172172A5051490033 @default.
• W2103172172 hasAuthorship W2103172172A5058372327 @default.
• W2103172172 hasAuthorship W2103172172A5063083094 @default.
• W2103172172 hasConcept C104317684 @default.
• W2103172172 hasConcept C136238340 @default.
• W2103172172 hasConcept C153911025 @default.
• W2103172172 hasConcept C155574463 @default.
• W2103172172 hasConcept C178790620 @default.
• W2103172172 hasConcept C185592680 @default.
• W2103172172 hasConcept C2779281246 @default.
• W2103172172 hasConcept C33161422 @default.
• W2103172172 hasConcept C40767141 @default.
• W2103172172 hasConcept C547475151 @default.
• W2103172172 hasConcept C55493867 @default.
• W2103172172 hasConcept C86803240 @default.
• W2103172172 hasConceptScore W2103172172C104317684 @default.
• W2103172172 hasConceptScore W2103172172C136238340 @default.
• W2103172172 hasConceptScore W2103172172C153911025 @default.
• W2103172172 hasConceptScore W2103172172C155574463 @default.
• W2103172172 hasConceptScore W2103172172C178790620 @default.
• W2103172172 hasConceptScore W2103172172C185592680 @default.
• W2103172172 hasConceptScore W2103172172C2779281246 @default.
• W2103172172 hasConceptScore W2103172172C33161422 @default.
• W2103172172 hasConceptScore W2103172172C40767141 @default.
• W2103172172 hasConceptScore W2103172172C547475151 @default.
• W2103172172 hasConceptScore W2103172172C55493867 @default.
• W2103172172 hasConceptScore W2103172172C86803240 @default.
• W2103172172 hasIssue "1" @default.
• W2103172172 hasLocation W21031721721 @default.
• W2103172172 hasLocation W21031721722 @default.
• W2103172172 hasOpenAccess W2103172172 @default.
• W2103172172 hasPrimaryLocation W21031721721 @default.
• W2103172172 hasRelatedWork W1521735421 @default.
• W2103172172 hasRelatedWork W2016628627 @default.
• W2103172172 hasRelatedWork W2274380954 @default.
• W2103172172 hasRelatedWork W2356177663 @default.
• W2103172172 hasRelatedWork W2359862199 @default.
• W2103172172 hasRelatedWork W2362223340 @default.
• W2103172172 hasRelatedWork W2372950376 @default.
• W2103172172 hasRelatedWork W2374424448 @default.
• W2103172172 hasRelatedWork W2380728338 @default.
• W2103172172 hasRelatedWork W4309884888 @default.
• W2103172172 hasVolume "86" @default.
• W2103172172 isParatext "false" @default.
• W2103172172 isRetracted "false" @default.

• next