FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103173218> ?p ?o ?g. }

• W2103173218 endingPage "702" @default.
• W2103173218 startingPage "685" @default.
• W2103173218 abstract "1. Using a solenoid-operated mixing device, time-resolved measurements were made of shortening and accompanying ATP hydrolysis at 20 degrees C by myofibrils prepared from rabbit psoas muscle. 2. The extent of ATP hydrolysis was determined by an improved Malachite Green method for determination of inorganic phosphate (Pi) in the presence of a large excess of ATP. For the measurement of the change in sarcomere length by phase contrast microscopy, shortening was terminated without delay and artifact by a mixture of 0.2 M-acetate (pH 4.6) and 1.25% (v/v) glutaraldehyde. 3. The shortening velocity per half-sarcomere was 10 microns s-1 in 25 mM-KCl for sarcomere lengths above 1.4 microns, and at least 12 microns s-1 in 150 mM-KCl for sarcomere lengths above 1.7 microns. During this rapid shortening, there was no significant ATP turnover by cross-bridges (upper 95% confidence limit: 0.14 mol (mol of myosin head)-1 in 25 mM-KCl; 0.12 mol mol-1 in KCl solutions greater than or equal to 100 mM). 4. When the sarcomeres shortened below 1.7 microns in KCl concentrations greater than 100 mM or below 1.4 microns in 25 mM-KCl, there was a transient acceleration of ATP hydrolysis (delayed ATP hydrolysis), which was then followed by a steady slow hydrolysis. 5. The magnitudes (+/- estimated standard deviation) of delayed ATP hydrolysis by myofibrils of initial sarcomere length 2.4 microns were 0.42 +/- 0.19, 0.31 +/- 0.10 and 0.17 +/- 0.09 mol (mol myosin head)-1 in 25 mM, 100 mM and 150 mM-KCl, respectively. For myofibrils of sarcomere length 2.0 microns, however, it decreased to 0.24 +/- 0.10 mol mol-1 in 25 mM-KCl or to an insignificant level in 150 mM-KCl. 6. These results indicate that most of the ATP hydrolysis products remain bound to cross-bridges during rapid shortening, and that when the force opposing shortening increases, a proportion of cross-bridges rapidly dissociate the products and enter the next ATP cycle, which diminishes with the decrease in shortening distance as well as the increase in ionic strength. Such behaviour of the cross-bridge is probably a manifestation of its energetic and kinetic properties in the state with bound ADP and Pi interacting with actin filaments at zero load and at a transition from zero to non-zero loads." @default.
• W2103173218 created "2016-06-24" @default.
• W2103173218 creator A5011794117 @default.
• W2103173218 creator A5026230089 @default.
• W2103173218 date "1991-09-01" @default.
• W2103173218 modified "2023-09-24" @default.
• W2103173218 title "Kinetics of adenosine triphosphate hydrolysis by shortening myofibrils from rabbit psoas muscle." @default.
• W2103173218 cites W1506043065 @default.
• W2103173218 cites W1890794327 @default.
• W2103173218 cites W1958593086 @default.
• W2103173218 cites W1963976991 @default.
• W2103173218 cites W1964846573 @default.
• W2103173218 cites W1980685237 @default.
• W2103173218 cites W1990901750 @default.
• W2103173218 cites W1998635013 @default.
• W2103173218 cites W2002527385 @default.
• W2103173218 cites W2006814619 @default.
• W2103173218 cites W2007456935 @default.
• W2103173218 cites W2022703044 @default.
• W2103173218 cites W2028957520 @default.
• W2103173218 cites W2041248923 @default.
• W2103173218 cites W2043397230 @default.
• W2103173218 cites W2044583931 @default.
• W2103173218 cites W2052976658 @default.
• W2103173218 cites W2061200435 @default.
• W2103173218 cites W2063390037 @default.
• W2103173218 cites W2072529314 @default.
• W2103173218 cites W2097314829 @default.
• W2103173218 cites W2097383319 @default.
• W2103173218 cites W2099472610 @default.
• W2103173218 cites W2104465251 @default.
• W2103173218 cites W2124754866 @default.
• W2103173218 cites W2127917887 @default.
• W2103173218 cites W2161375436 @default.
• W2103173218 cites W2164647651 @default.
• W2103173218 cites W2166488402 @default.
• W2103173218 cites W2167239763 @default.
• W2103173218 cites W2169400659 @default.
• W2103173218 cites W2194833841 @default.
• W2103173218 cites W2444098044 @default.
• W2103173218 cites W789458867 @default.
• W2103173218 doi "https://doi.org/10.1113/jphysiol.1991.sp018773" @default.
• W2103173218 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1180220" @default.
• W2103173218 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1816389" @default.
• W2103173218 hasPublicationYear "1991" @default.
• W2103173218 type Work @default.
• W2103173218 sameAs 2103173218 @default.
• W2103173218 citedByCount "42" @default.
• W2103173218 countsByYear W21031732182012 @default.
• W2103173218 countsByYear W21031732182013 @default.
• W2103173218 countsByYear W21031732182016 @default.
• W2103173218 countsByYear W21031732182017 @default.
• W2103173218 countsByYear W21031732182018 @default.
• W2103173218 crossrefType "journal-article" @default.
• W2103173218 hasAuthorship W2103173218A5011794117 @default.
• W2103173218 hasAuthorship W2103173218A5026230089 @default.
• W2103173218 hasBestOaLocation W21031732182 @default.
• W2103173218 hasConcept C113196181 @default.
• W2103173218 hasConcept C134018914 @default.
• W2103173218 hasConcept C141315368 @default.
• W2103173218 hasConcept C181199279 @default.
• W2103173218 hasConcept C185592680 @default.
• W2103173218 hasConcept C207200792 @default.
• W2103173218 hasConcept C23265538 @default.
• W2103173218 hasConcept C43617362 @default.
• W2103173218 hasConcept C55493867 @default.
• W2103173218 hasConcept C68731436 @default.
• W2103173218 hasConcept C71924100 @default.
• W2103173218 hasConcept C7301908 @default.
• W2103173218 hasConcept C94412978 @default.
• W2103173218 hasConceptScore W2103173218C113196181 @default.
• W2103173218 hasConceptScore W2103173218C134018914 @default.
• W2103173218 hasConceptScore W2103173218C141315368 @default.
• W2103173218 hasConceptScore W2103173218C181199279 @default.
• W2103173218 hasConceptScore W2103173218C185592680 @default.
• W2103173218 hasConceptScore W2103173218C207200792 @default.
• W2103173218 hasConceptScore W2103173218C23265538 @default.
• W2103173218 hasConceptScore W2103173218C43617362 @default.
• W2103173218 hasConceptScore W2103173218C55493867 @default.
• W2103173218 hasConceptScore W2103173218C68731436 @default.
• W2103173218 hasConceptScore W2103173218C71924100 @default.
• W2103173218 hasConceptScore W2103173218C7301908 @default.
• W2103173218 hasConceptScore W2103173218C94412978 @default.
• W2103173218 hasIssue "1" @default.
• W2103173218 hasLocation W21031732181 @default.
• W2103173218 hasLocation W21031732182 @default.
• W2103173218 hasLocation W21031732183 @default.
• W2103173218 hasOpenAccess W2103173218 @default.
• W2103173218 hasPrimaryLocation W21031732181 @default.
• W2103173218 hasRelatedWork W1536566630 @default.
• W2103173218 hasRelatedWork W1753238626 @default.
• W2103173218 hasRelatedWork W2019679133 @default.
• W2103173218 hasRelatedWork W2019974411 @default.
• W2103173218 hasRelatedWork W2103173218 @default.
• W2103173218 hasRelatedWork W2114424130 @default.
• W2103173218 hasRelatedWork W2128601601 @default.
• W2103173218 hasRelatedWork W2463287281 @default.
• W2103173218 hasRelatedWork W2601406649 @default.

• next