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- W2103178575 abstract "The Arabidopsis thaliana chloroplast contains 20 group-II introns in its genome, and seven known splicing factors are required for the splicing of overlapping subsets of 19 of them. We describe an additional protein (OTP51) that specifically promotes the splicing of the only group-II intron for which no splicing factor has been described previously. This protein is a pentatricopeptide repeat (PPR) protein containing two LAGLIDADG motifs found in group-I intron maturases in other organisms. Amino acids thought to be important for the homing endonuclease activity of other LAGLIDADG proteins are missing in this protein, but the amino acids described to be important for maturase activity are conserved. OTP51 is absolutely required for the splicing of ycf3 intron 2, and also influences the splicing of several other group-IIa introns. Loss of OTP51 has far-reaching consequences for photosystem-I and photosystem-II assembly, and for the photosynthetic fluorescence characteristics of mutant plants." @default.
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- W2103178575 date "2008-10-01" @default.
- W2103178575 modified "2023-10-16" @default.
- W2103178575 title "The pentatricopeptide repeat gene<i>OTP51</i>with two LAGLIDADG motifs is required for the<i>cis</i>-splicing of plastid<i>ycf3</i>intron 2 in<i>Arabidopsis thaliana</i>" @default.
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- W2103178575 doi "https://doi.org/10.1111/j.1365-313x.2008.03581.x" @default.
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