FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103201163> ?p ?o ?g. }

• W2103201163 endingPage "389" @default.
• W2103201163 startingPage "377" @default.
• W2103201163 abstract "The first 10 residues within the Src homology domain (SH)-4 domain of the Src family kinase Fyn are required for binding to the immune receptor tyrosine-based activation motif (ITAM) of T cell receptor (TCR) subunits. Recently, mutation of glycine 2, cysteine 3, and lysines 7 and 9 was shown to block binding of Fyn to TCR zeta chain ITAMs, prompting the designation of these residues as an ITAM recognition motif (Gauen, L.K.T., M.E. Linder, and A.S. Shaw. 1996. J. Cell Biol. 133:1007-1015). Here we show that these residues do not mediate direct interactions with TCR ITAMs, but rather are required for efficient myristoylation and palmitoylation of Fyn. Specifically, coexpression of a K7,9A-Fyn mutant with N-myristoyltransferase restored myristoylation, membrane binding, and association with the cytoplasmic tail of TCR zeta fused to CD8. Conversely, treatment of cells with 2-hydroxymyristate, a myristoylation inhibitor, blocked association of wild-type Fyn with zeta. The Fyn NH2 terminus was necessary but not sufficient for interaction with zeta and both Fyn kinase and SH2 domains were required, directing phosphorylation of zeta ITAM tyrosines and binding to zeta ITAM phosphotyrosines. Fyn/zeta interaction was sensitive to octylglucoside and filipin, agents that disrupt membrane rafts. Moreover, a plasma membrane bound, farnesylated Fyn construct, G2A,C3S-FynKRas, was not enriched in the detergent insoluble fraction and did not associate with zeta. We conclude that the Fyn SH4 domain provides the signals for fatty acylation and specific plasma membrane localization, stabilizing the interactions between the Fyn SH2 domain and phosphotyrosines in TCR zeta chain ITAMs." @default.
• W2103201163 created "2016-06-24" @default.
• W2103201163 creator A5007994479 @default.
• W2103201163 creator A5027044141 @default.
• W2103201163 date "1999-04-19" @default.
• W2103201163 modified "2023-10-13" @default.
• W2103201163 title "Dual Fatty Acylation of p59<i>Fyn</i> Is Required for Association with the T Cell Receptor ζ Chain through Phosphotyrosine–Src Homology Domain-2 Interactions" @default.
• W2103201163 cites W1494227533 @default.
• W2103201163 cites W1512968077 @default.
• W2103201163 cites W1529387078 @default.
• W2103201163 cites W1609919210 @default.
• W2103201163 cites W1806723516 @default.
• W2103201163 cites W1817318311 @default.
• W2103201163 cites W1965336618 @default.
• W2103201163 cites W1980004909 @default.
• W2103201163 cites W1992910677 @default.
• W2103201163 cites W1995299045 @default.
• W2103201163 cites W1998865212 @default.
• W2103201163 cites W2009009539 @default.
• W2103201163 cites W2010207338 @default.
• W2103201163 cites W2010439047 @default.
• W2103201163 cites W2012694659 @default.
• W2103201163 cites W2020279663 @default.
• W2103201163 cites W2025409698 @default.
• W2103201163 cites W2030202169 @default.
• W2103201163 cites W2032062053 @default.
• W2103201163 cites W2037275527 @default.
• W2103201163 cites W2044409894 @default.
• W2103201163 cites W2057687973 @default.
• W2103201163 cites W2060343311 @default.
• W2103201163 cites W2068504553 @default.
• W2103201163 cites W2072241649 @default.
• W2103201163 cites W2072278904 @default.
• W2103201163 cites W2073869452 @default.
• W2103201163 cites W2087983099 @default.
• W2103201163 cites W2092296126 @default.
• W2103201163 cites W2094126922 @default.
• W2103201163 cites W2101257013 @default.
• W2103201163 cites W2104901736 @default.
• W2103201163 cites W2106230884 @default.
• W2103201163 cites W2114844324 @default.
• W2103201163 cites W2115037258 @default.
• W2103201163 cites W2121936687 @default.
• W2103201163 cites W2127444703 @default.
• W2103201163 cites W2129558898 @default.
• W2103201163 cites W2133796240 @default.
• W2103201163 cites W2141367941 @default.
• W2103201163 cites W2151038122 @default.
• W2103201163 cites W2156633091 @default.
• W2103201163 cites W2161314348 @default.
• W2103201163 cites W2166185276 @default.
• W2103201163 cites W2169803769 @default.
• W2103201163 cites W2180245338 @default.
• W2103201163 cites W4236525885 @default.
• W2103201163 cites W95054676 @default.
• W2103201163 doi "https://doi.org/10.1083/jcb.145.2.377" @default.
• W2103201163 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2133112" @default.
• W2103201163 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10209031" @default.
• W2103201163 hasPublicationYear "1999" @default.
• W2103201163 type Work @default.
• W2103201163 sameAs 2103201163 @default.
• W2103201163 citedByCount "103" @default.
• W2103201163 countsByYear W21032011632013 @default.
• W2103201163 countsByYear W21032011632014 @default.
• W2103201163 countsByYear W21032011632015 @default.
• W2103201163 countsByYear W21032011632016 @default.
• W2103201163 countsByYear W21032011632017 @default.
• W2103201163 countsByYear W21032011632018 @default.
• W2103201163 countsByYear W21032011632019 @default.
• W2103201163 countsByYear W21032011632020 @default.
• W2103201163 countsByYear W21032011632021 @default.
• W2103201163 countsByYear W21032011632022 @default.
• W2103201163 crossrefType "journal-article" @default.
• W2103201163 hasAuthorship W2103201163A5007994479 @default.
• W2103201163 hasAuthorship W2103201163A5027044141 @default.
• W2103201163 hasBestOaLocation W21032011631 @default.
• W2103201163 hasConcept C108636557 @default.
• W2103201163 hasConcept C11960822 @default.
• W2103201163 hasConcept C146727910 @default.
• W2103201163 hasConcept C181199279 @default.
• W2103201163 hasConcept C197153747 @default.
• W2103201163 hasConcept C2776165026 @default.
• W2103201163 hasConcept C2779201268 @default.
• W2103201163 hasConcept C2779976819 @default.
• W2103201163 hasConcept C29907709 @default.
• W2103201163 hasConcept C37686147 @default.
• W2103201163 hasConcept C42362537 @default.
• W2103201163 hasConcept C55493867 @default.
• W2103201163 hasConcept C62478195 @default.
• W2103201163 hasConcept C77207865 @default.
• W2103201163 hasConcept C86803240 @default.
• W2103201163 hasConcept C95444343 @default.
• W2103201163 hasConceptScore W2103201163C108636557 @default.
• W2103201163 hasConceptScore W2103201163C11960822 @default.
• W2103201163 hasConceptScore W2103201163C146727910 @default.
• W2103201163 hasConceptScore W2103201163C181199279 @default.
• W2103201163 hasConceptScore W2103201163C197153747 @default.
• W2103201163 hasConceptScore W2103201163C2776165026 @default.

• next