FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103238040> ?p ?o ?g. }

• W2103238040 endingPage "491" @default.
• W2103238040 startingPage "484" @default.
• W2103238040 abstract "Normal skin contains no epidermal calprotectin. In biopsies from various inflammatory skin disorders, however, this antimicrobial protein occurs in the cytoplasm of keratinocytes.To exclude the possibility of epidermal uptake of calprotectin from granulocytes and macrophages in diseased skin, we investigated whether cytokine-stimulated human keratinocytes can express calprotectin in vitro.Keratinocytes from healthy individuals were cultured in serum-free keratinocyte medium. The cells were stimulated with different cytokines [interferon (IFN)-gamma, tumour necrosis factor (TNF)-alpha, interleukin (IL)-1beta, IL-10 and IL-13], both separately and in various combinations. Cytoplasmic protein levels of calprotectin were measured by an enzyme-linked immunosorbent assay performed on fixed adherent keratinocytes, and mRNA expression was determined by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR).Calprotectin was produced by cytokine-stimulated keratinocytes, especially in response to combinations of the proinflammatory cytokines, which showed an additive upregulatory effect. When expression of mRNA for the light (MRP-8) and heavy (MRP-14) calprotectin chain was determined by RT-PCR, their respective levels were shown to be increased four- to ninefold and three- to fivefold after 24 h of combined stimulation with IFN-gamma and TNF-alpha. The time course of calprotectin production showed no significant elevation for the first 16 h but then increased and peaked after 36 h.Cultured human keratinocytes stimulated with proinflammatory cytokines produce calprotectin, suggesting that epidermal expression of this antimicrobial protein in diseased skin reflects compartmentalized synthesis rather than uptake from dermal inflammatory cells." @default.
• W2103238040 created "2016-06-24" @default.
• W2103238040 creator A5002643332 @default.
• W2103238040 creator A5037734937 @default.
• W2103238040 creator A5042575652 @default.
• W2103238040 creator A5050151650 @default.
• W2103238040 creator A5054926053 @default.
• W2103238040 date "2003-09-01" @default.
• W2103238040 modified "2023-09-25" @default.
• W2103238040 title "Proinflammatory cytokines upregulate expression of calprotectin (L1 protein, MRP-8/MRP-14) in cultured human keratinocytes" @default.
• W2103238040 cites W1499500424 @default.
• W2103238040 cites W1660611080 @default.
• W2103238040 cites W1925392529 @default.
• W2103238040 cites W1974861015 @default.
• W2103238040 cites W1977957923 @default.
• W2103238040 cites W1997236045 @default.
• W2103238040 cites W1998821944 @default.
• W2103238040 cites W2001727356 @default.
• W2103238040 cites W2007286072 @default.
• W2103238040 cites W2018728707 @default.
• W2103238040 cites W2021873282 @default.
• W2103238040 cites W2047325249 @default.
• W2103238040 cites W2057797562 @default.
• W2103238040 cites W2060840023 @default.
• W2103238040 cites W2060866852 @default.
• W2103238040 cites W2064540741 @default.
• W2103238040 cites W2079495761 @default.
• W2103238040 cites W2081512173 @default.
• W2103238040 cites W2085341927 @default.
• W2103238040 cites W2087418912 @default.
• W2103238040 cites W2093692047 @default.
• W2103238040 cites W2098053923 @default.
• W2103238040 cites W2102531596 @default.
• W2103238040 cites W2126954877 @default.
• W2103238040 cites W2133955925 @default.
• W2103238040 cites W2137323762 @default.
• W2103238040 cites W2155379600 @default.
• W2103238040 cites W2163417989 @default.
• W2103238040 cites W2318679016 @default.
• W2103238040 cites W2345764307 @default.
• W2103238040 doi "https://doi.org/10.1046/j.1365-2133.2003.05536.x" @default.
• W2103238040 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14510979" @default.
• W2103238040 hasPublicationYear "2003" @default.
• W2103238040 type Work @default.
• W2103238040 sameAs 2103238040 @default.
• W2103238040 citedByCount "54" @default.
• W2103238040 countsByYear W21032380402012 @default.
• W2103238040 countsByYear W21032380402013 @default.
• W2103238040 countsByYear W21032380402014 @default.
• W2103238040 countsByYear W21032380402015 @default.
• W2103238040 countsByYear W21032380402016 @default.
• W2103238040 countsByYear W21032380402017 @default.
• W2103238040 countsByYear W21032380402018 @default.
• W2103238040 countsByYear W21032380402019 @default.
• W2103238040 countsByYear W21032380402020 @default.
• W2103238040 countsByYear W21032380402021 @default.
• W2103238040 countsByYear W21032380402022 @default.
• W2103238040 countsByYear W21032380402023 @default.
• W2103238040 crossrefType "journal-article" @default.
• W2103238040 hasAuthorship W2103238040A5002643332 @default.
• W2103238040 hasAuthorship W2103238040A5037734937 @default.
• W2103238040 hasAuthorship W2103238040A5042575652 @default.
• W2103238040 hasAuthorship W2103238040A5050151650 @default.
• W2103238040 hasAuthorship W2103238040A5054926053 @default.
• W2103238040 hasConcept C104317684 @default.
• W2103238040 hasConcept C105580179 @default.
• W2103238040 hasConcept C126322002 @default.
• W2103238040 hasConcept C127561419 @default.
• W2103238040 hasConcept C153911025 @default.
• W2103238040 hasConcept C164027704 @default.
• W2103238040 hasConcept C17991360 @default.
• W2103238040 hasConcept C185592680 @default.
• W2103238040 hasConcept C202751555 @default.
• W2103238040 hasConcept C203014093 @default.
• W2103238040 hasConcept C2776914184 @default.
• W2103238040 hasConcept C2777074287 @default.
• W2103238040 hasConcept C2778260677 @default.
• W2103238040 hasConcept C2778292693 @default.
• W2103238040 hasConcept C2778557267 @default.
• W2103238040 hasConcept C2778690821 @default.
• W2103238040 hasConcept C2779134260 @default.
• W2103238040 hasConcept C2780841837 @default.
• W2103238040 hasConcept C4692481 @default.
• W2103238040 hasConcept C553184892 @default.
• W2103238040 hasConcept C55493867 @default.
• W2103238040 hasConcept C71924100 @default.
• W2103238040 hasConcept C74172505 @default.
• W2103238040 hasConcept C86803240 @default.
• W2103238040 hasConceptScore W2103238040C104317684 @default.
• W2103238040 hasConceptScore W2103238040C105580179 @default.
• W2103238040 hasConceptScore W2103238040C126322002 @default.
• W2103238040 hasConceptScore W2103238040C127561419 @default.
• W2103238040 hasConceptScore W2103238040C153911025 @default.
• W2103238040 hasConceptScore W2103238040C164027704 @default.
• W2103238040 hasConceptScore W2103238040C17991360 @default.
• W2103238040 hasConceptScore W2103238040C185592680 @default.
• W2103238040 hasConceptScore W2103238040C202751555 @default.
• W2103238040 hasConceptScore W2103238040C203014093 @default.

• next