Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103242123> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2103242123 endingPage "1016" @default.
- W2103242123 startingPage "1008" @default.
- W2103242123 abstract "Kinases are key targets for drug discovery. In the field of screening in general and especially in the kinase area, because of considerations of efficiency and cost, radioactivity-based assays tend to be replaced by alternative, mostly fluorescence-based, assays. Today, the limiting factor is rarely the number of data points that can be obtained but rather the quality of the data, enzyme availability, and cost. In this article, the authors describe the development of an assay for a kinase screen based on the electrophoretic separation of fluorescent product and substrate using a Caliper-based nanofluidics environment in on-chip incubation mode. The authors present the results of screening a focused set of 32,000 compounds together with confirmation data obtained in a filtration assay. In addition, they have made a small-scale comparison between the on-chip and off-chip nanofluidics screening modes. In their hands, the screen in on-chip mode is characterized by high precision most likely due to the absence of liquid pipetting; an excellent confirmation rate (62%) in an independent assay format, namely, filtration; and good sensitivity. This study led to the identification of 4 novel chemical series of inhibitors." @default.
- W2103242123 created "2016-06-24" @default.
- W2103242123 creator A5023891526 @default.
- W2103242123 creator A5042753215 @default.
- W2103242123 creator A5085706199 @default.
- W2103242123 date "2009-09-01" @default.
- W2103242123 modified "2023-10-16" @default.
- W2103242123 title "Correction for Interference by Test Samples in High-Throughput Assays" @default.
- W2103242123 cites W2006370393 @default.
- W2103242123 cites W2013655852 @default.
- W2103242123 cites W2036194106 @default.
- W2103242123 cites W2036922942 @default.
- W2103242123 cites W2051047054 @default.
- W2103242123 cites W2059672514 @default.
- W2103242123 cites W2065915163 @default.
- W2103242123 cites W2076643163 @default.
- W2103242123 cites W2087396965 @default.
- W2103242123 cites W2123896578 @default.
- W2103242123 cites W2125920799 @default.
- W2103242123 cites W2159907048 @default.
- W2103242123 cites W2166393666 @default.
- W2103242123 doi "https://doi.org/10.1177/1087057106286653" @default.
- W2103242123 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16751332" @default.
- W2103242123 hasPublicationYear "2009" @default.
- W2103242123 type Work @default.
- W2103242123 sameAs 2103242123 @default.
- W2103242123 citedByCount "49" @default.
- W2103242123 countsByYear W21032421232012 @default.
- W2103242123 countsByYear W21032421232013 @default.
- W2103242123 countsByYear W21032421232014 @default.
- W2103242123 countsByYear W21032421232015 @default.
- W2103242123 countsByYear W21032421232016 @default.
- W2103242123 countsByYear W21032421232018 @default.
- W2103242123 countsByYear W21032421232019 @default.
- W2103242123 countsByYear W21032421232020 @default.
- W2103242123 countsByYear W21032421232021 @default.
- W2103242123 countsByYear W21032421232022 @default.
- W2103242123 countsByYear W21032421232023 @default.
- W2103242123 crossrefType "journal-article" @default.
- W2103242123 hasAuthorship W2103242123A5023891526 @default.
- W2103242123 hasAuthorship W2103242123A5042753215 @default.
- W2103242123 hasAuthorship W2103242123A5085706199 @default.
- W2103242123 hasBestOaLocation W21032421231 @default.
- W2103242123 hasConcept C165005293 @default.
- W2103242123 hasConcept C171250308 @default.
- W2103242123 hasConcept C185592680 @default.
- W2103242123 hasConcept C192562407 @default.
- W2103242123 hasConcept C41008148 @default.
- W2103242123 hasConcept C43617362 @default.
- W2103242123 hasConcept C51323132 @default.
- W2103242123 hasConcept C55493867 @default.
- W2103242123 hasConcept C70721500 @default.
- W2103242123 hasConcept C74187038 @default.
- W2103242123 hasConcept C76155785 @default.
- W2103242123 hasConcept C86803240 @default.
- W2103242123 hasConceptScore W2103242123C165005293 @default.
- W2103242123 hasConceptScore W2103242123C171250308 @default.
- W2103242123 hasConceptScore W2103242123C185592680 @default.
- W2103242123 hasConceptScore W2103242123C192562407 @default.
- W2103242123 hasConceptScore W2103242123C41008148 @default.
- W2103242123 hasConceptScore W2103242123C43617362 @default.
- W2103242123 hasConceptScore W2103242123C51323132 @default.
- W2103242123 hasConceptScore W2103242123C55493867 @default.
- W2103242123 hasConceptScore W2103242123C70721500 @default.
- W2103242123 hasConceptScore W2103242123C74187038 @default.
- W2103242123 hasConceptScore W2103242123C76155785 @default.
- W2103242123 hasConceptScore W2103242123C86803240 @default.
- W2103242123 hasIssue "8" @default.
- W2103242123 hasLocation W21032421231 @default.
- W2103242123 hasLocation W21032421232 @default.
- W2103242123 hasLocation W21032421233 @default.
- W2103242123 hasOpenAccess W2103242123 @default.
- W2103242123 hasPrimaryLocation W21032421231 @default.
- W2103242123 hasRelatedWork W1475153 @default.
- W2103242123 hasRelatedWork W1865162173 @default.
- W2103242123 hasRelatedWork W1981076832 @default.
- W2103242123 hasRelatedWork W2001717001 @default.
- W2103242123 hasRelatedWork W2013003323 @default.
- W2103242123 hasRelatedWork W2024773645 @default.
- W2103242123 hasRelatedWork W2086566345 @default.
- W2103242123 hasRelatedWork W2124910405 @default.
- W2103242123 hasRelatedWork W2551232629 @default.
- W2103242123 hasRelatedWork W2614037532 @default.
- W2103242123 hasVolume "14" @default.
- W2103242123 isParatext "false" @default.
- W2103242123 isRetracted "false" @default.
- W2103242123 magId "2103242123" @default.
- W2103242123 workType "article" @default.