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• W2103245013 endingPage "1543" @default.
• W2103245013 startingPage "1533" @default.
• W2103245013 abstract "ABSTRACT Centromere-specific H3-like proteins (CenH3s) are conserved across the eukaryotic kingdom and are required for packaging centromere DNA into a specialized chromatin structure required for kinetochore assembly. Cse4 is the CenH3 protein of the budding yeast Saccharomyces cerevisiae . Like all CenH3 proteins, Cse4 consists of a conserved histone fold domain (HFD) and a divergent N terminus (NT). The Cse4 NT contains an essential domain designated END (for essential N-terminal domain); deletion of END is lethal. To investigate the role of the Cse4 NT in centromere targeting, a series of deletion alleles ( cse4 Δ NT ) were analyzed. No part of the Cse4 NT was required to target mutant proteins to centromere DNA in the presence of functional Cse4. A Cse4 degron strain was used to examine targeting of a Cse4ΔNT protein in the absence of wild-type Cse4. The END was not required for centromere targeting under these conditions, confirming that the HFD confers specificity of Cse4 centromere targeting. Surprisingly, overexpression of the HFD bypassed the requirement for the END altogether, and viable S. cerevisiae strains in which the cells express only the Cse4 HFD and six adjacent N-terminal amino acids (Cse4Δ129) were constructed. Despite the complete absence of the NT, mitotic chromosome loss in the cse4 Δ 129 strain increased only 6-fold compared to a 15-fold increase in strains overexpressing wild-type Cse4. Thus, when overexpressed, the Cse4 HFD is sufficient for centromere function in S. cerevisiae , and no posttranslational modification or interaction of the NT with other kinetochore component(s) is essential for accurate chromosome segregation in budding yeast." @default.
• W2103245013 created "2016-06-24" @default.
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• W2103245013 date "2004-12-01" @default.
• W2103245013 modified "2023-10-01" @default.
• W2103245013 title "The Histone Fold Domain of Cse4 Is Sufficient for <i>CEN</i> Targeting and Propagation of Active Centromeres in Budding Yeast" @default.
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• W2103245013 doi "https://doi.org/10.1128/ec.3.6.1533-1543.2004" @default.
• W2103245013 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/539035" @default.
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