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• W2103258246 abstract "The insecticidal properties of biotin-binding proteins (BBPs) have recently been exploited in transgenic plants. As BBPs have a broad spectrum of insect toxicity, their potential impacts on non-target in- sects such as honey bees need to be assessed. In this study, the effects of feeding a purified BBP, avidin, to honey bee larvae and adults were determined. A realistic larval dosing regime was developed by estimating the pollen content of brood food in the field and adding avidin to artificial diet at rates that simulated the pres- ence of avidin-expressing transgenic pollen in brood food. Larval survival and development were unaffected by avidin in assays which simulated larvae receiving pollen expressing 0, 4 or 40 µM avidin at concentrations of 164 µg pollen per mg food for the first 2 days and 880 µg pollen per mg food thereafter. Food consumption and survival of adult bees were also unaffected by avidin added to pollen-candy at levels corresponding to pol- len expression of 0, 6.7 or 20 µM avidin. Apis mellifera / biotin-binding protein / avidin / transgenic plant" @default.
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• W2103258246 date "2002-09-01" @default.
• W2103258246 modified "2023-10-05" @default.
• W2103258246 title "Effects of ingestion of a biotin-binding protein on adult and larval honey bees" @default.
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• W2103258246 doi "https://doi.org/10.1051/apido:2002030" @default.
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