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- W2103261033 abstract "Abstract Rapid‐mixing, rapid‐freezing techniques are employed for the study of enzyme or chemical catalytic mechanisms. The techniques aim to provide electronic and molecular structures of transient intermediates formed during the reaction by means of spectroscopic analyses of the rapidly frozen samples. Briefly, the mixing‐sampling procedure consists of the rapid mixing of two reactants, for example, enzyme and substrate, followed by rapid freezing of the mixture in a liquid cryo‐medium or on a cold plate to quench the reaction progress. The resulting frozen powder is subsequently sampled and made available for further low‐temperature spectroscopic analyses such as UV‐Vis spectroscopy, multifrequency Electron Paramagnetic Resonance (EPR) spectroscopy, Electron Nuclear Double Resonance (ENDOR) spectroscopy, Electron Spin Echo Envelope Modulation (ESEEM), Magnetic Circular Dichroïsm (MCD) spectroscopy, Mössbauer spectroscopy, resonance Raman spectroscopy, or X‐ray Absorption Spectroscopy. By preparing a series of samples freeze‐quenched after various times, a full kinetic profile of the catalytic cycle of an enzyme or any other chemical reaction is obtained. The structures of intermediates are assigned in conjunction with (a selection of) the spectroscopic techniques listed above. This article details various aspects of continuous‐flow and stopped‐flow rapid‐mixing techniques, considering mixer design, freeze‐quenching methodology, and sampling. The scope and limitations to (bio)chemical research of the well‐established rapid freeze‐quench (RFQ) and the recently developed microsecond freeze‐hyperquenching (MHQ) technologies are specifically highlighted." @default.
- W2103261033 created "2016-06-24" @default.
- W2103261033 creator A5082841450 @default.
- W2103261033 date "2005-09-07" @default.
- W2103261033 modified "2023-09-28" @default.
- W2103261033 title "Freeze-Quench Kinetics" @default.
- W2103261033 cites W1034335324 @default.
- W2103261033 cites W1258063941 @default.
- W2103261033 cites W1531102271 @default.
- W2103261033 cites W1639810208 @default.
- W2103261033 cites W1668976164 @default.
- W2103261033 cites W1876379064 @default.
- W2103261033 cites W1963598011 @default.
- W2103261033 cites W1964767087 @default.
- W2103261033 cites W1967211277 @default.
- W2103261033 cites W1967614519 @default.
- W2103261033 cites W1968172212 @default.
- W2103261033 cites W1970206530 @default.
- W2103261033 cites W1970645077 @default.
- W2103261033 cites W1972905772 @default.
- W2103261033 cites W1975262546 @default.
- W2103261033 cites W1976790269 @default.
- W2103261033 cites W1978783094 @default.
- W2103261033 cites W1979366638 @default.
- W2103261033 cites W1984116682 @default.
- W2103261033 cites W1985370802 @default.
- W2103261033 cites W1985703543 @default.
- W2103261033 cites W1987575621 @default.
- W2103261033 cites W1989744091 @default.
- W2103261033 cites W1993463705 @default.
- W2103261033 cites W1993758547 @default.
- W2103261033 cites W1993871972 @default.
- W2103261033 cites W1996267771 @default.
- W2103261033 cites W1998385854 @default.
- W2103261033 cites W1998640425 @default.
- W2103261033 cites W2001092115 @default.
- W2103261033 cites W2002415493 @default.
- W2103261033 cites W2002612346 @default.
- W2103261033 cites W2002697193 @default.
- W2103261033 cites W2007464478 @default.
- W2103261033 cites W2009170003 @default.
- W2103261033 cites W2014288844 @default.
- W2103261033 cites W2018063951 @default.
- W2103261033 cites W2024297523 @default.
- W2103261033 cites W2024477147 @default.
- W2103261033 cites W2024794991 @default.
- W2103261033 cites W2025088751 @default.
- W2103261033 cites W2025325690 @default.
- W2103261033 cites W2025577803 @default.
- W2103261033 cites W2028954835 @default.
- W2103261033 cites W2029552331 @default.
- W2103261033 cites W2032998875 @default.
- W2103261033 cites W2033503314 @default.
- W2103261033 cites W2034523602 @default.
- W2103261033 cites W2035602472 @default.
- W2103261033 cites W2037193428 @default.
- W2103261033 cites W2038128938 @default.
- W2103261033 cites W2042600398 @default.
- W2103261033 cites W2044375066 @default.
- W2103261033 cites W2045173898 @default.
- W2103261033 cites W2045548346 @default.
- W2103261033 cites W2047391469 @default.
- W2103261033 cites W2051666694 @default.
- W2103261033 cites W2052090947 @default.
- W2103261033 cites W2052642907 @default.
- W2103261033 cites W2054418709 @default.
- W2103261033 cites W2058799785 @default.
- W2103261033 cites W2059134779 @default.
- W2103261033 cites W2059464793 @default.
- W2103261033 cites W2062716800 @default.
- W2103261033 cites W2066518689 @default.
- W2103261033 cites W2068018042 @default.
- W2103261033 cites W2069417563 @default.
- W2103261033 cites W2069523477 @default.
- W2103261033 cites W2071331502 @default.
- W2103261033 cites W2072975767 @default.
- W2103261033 cites W2074037790 @default.
- W2103261033 cites W2075364246 @default.
- W2103261033 cites W2076563340 @default.
- W2103261033 cites W2077621515 @default.
- W2103261033 cites W2078358974 @default.
- W2103261033 cites W2078537861 @default.
- W2103261033 cites W2078770229 @default.
- W2103261033 cites W2082627224 @default.
- W2103261033 cites W2086147321 @default.
- W2103261033 cites W2086182124 @default.
- W2103261033 cites W2087131278 @default.
- W2103261033 cites W2087629000 @default.
- W2103261033 cites W2090623494 @default.
- W2103261033 cites W2092774147 @default.
- W2103261033 cites W2093490732 @default.
- W2103261033 cites W2094119649 @default.
- W2103261033 cites W2098942915 @default.
- W2103261033 cites W2100141723 @default.
- W2103261033 cites W2102074396 @default.
- W2103261033 cites W2105059492 @default.
- W2103261033 cites W2110116688 @default.
- W2103261033 cites W2110556690 @default.
- W2103261033 cites W2113645496 @default.
- W2103261033 cites W2114782293 @default.