Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103265200> ?p ?o ?g. }
- W2103265200 endingPage "e1002540" @default.
- W2103265200 startingPage "e1002540" @default.
- W2103265200 abstract "A CpG island (CGI) lies at the 5′ end of the Airn macro non-protein-coding (nc) RNA that represses the flanking Igf2r promoter in cis on paternally inherited chromosomes. In addition to being modified on maternally inherited chromosomes by a DNA methylation imprint, the Airn CGI shows two unusual organization features: its position immediately downstream of the Airn promoter and transcription start site and a series of tandem direct repeats (TDRs) occupying its second half. The physical separation of the Airn promoter from the CGI provides a model to investigate if the CGI plays distinct transcriptional and epigenetic roles. We used homologous recombination to generate embryonic stem cells carrying deletions at the endogenous locus of the entire CGI or just the TDRs. The deleted Airn alleles were analyzed by using an ES cell imprinting model that recapitulates the onset of Igf2r imprinted expression in embryonic development or by using knock-out mice. The results show that the CGI is required for efficient Airn initiation and to maintain the unmethylated state of the Airn promoter, which are both necessary for Igf2r repression on the paternal chromosome. The TDRs occupying the second half of the CGI play a minor role in Airn transcriptional elongation or processivity, but are essential for methylation on the maternal Airn promoter that is necessary for Igf2r to be expressed from this chromosome. Together the data indicate the existence of a class of regulatory CGIs in the mammalian genome that act downstream of the promoter and transcription start." @default.
- W2103265200 created "2016-06-24" @default.
- W2103265200 creator A5007091163 @default.
- W2103265200 creator A5008675802 @default.
- W2103265200 creator A5012945814 @default.
- W2103265200 creator A5013689988 @default.
- W2103265200 creator A5018457785 @default.
- W2103265200 creator A5018619035 @default.
- W2103265200 creator A5021220729 @default.
- W2103265200 creator A5023312986 @default.
- W2103265200 creator A5040833749 @default.
- W2103265200 creator A5041138853 @default.
- W2103265200 creator A5046008529 @default.
- W2103265200 creator A5048756693 @default.
- W2103265200 creator A5053441352 @default.
- W2103265200 creator A5067036233 @default.
- W2103265200 creator A5073943786 @default.
- W2103265200 date "2012-03-01" @default.
- W2103265200 modified "2023-10-17" @default.
- W2103265200 title "A Downstream CpG Island Controls Transcript Initiation and Elongation and the Methylation State of the Imprinted Airn Macro ncRNA Promoter" @default.
- W2103265200 cites W1558013374 @default.
- W2103265200 cites W1609534144 @default.
- W2103265200 cites W1959319781 @default.
- W2103265200 cites W1964155739 @default.
- W2103265200 cites W1964789204 @default.
- W2103265200 cites W1970488458 @default.
- W2103265200 cites W1978538506 @default.
- W2103265200 cites W1980273804 @default.
- W2103265200 cites W1981354816 @default.
- W2103265200 cites W1985481176 @default.
- W2103265200 cites W1986388692 @default.
- W2103265200 cites W1988551012 @default.
- W2103265200 cites W1990117400 @default.
- W2103265200 cites W1992658017 @default.
- W2103265200 cites W1993063708 @default.
- W2103265200 cites W1993470489 @default.
- W2103265200 cites W1998964174 @default.
- W2103265200 cites W1999613082 @default.
- W2103265200 cites W2001043267 @default.
- W2103265200 cites W2001206796 @default.
- W2103265200 cites W2001210792 @default.
- W2103265200 cites W2009940298 @default.
- W2103265200 cites W2010291571 @default.
- W2103265200 cites W2013961335 @default.
- W2103265200 cites W2019820997 @default.
- W2103265200 cites W2021309463 @default.
- W2103265200 cites W2022867908 @default.
- W2103265200 cites W2023373744 @default.
- W2103265200 cites W2025310562 @default.
- W2103265200 cites W2030487003 @default.
- W2103265200 cites W2034025977 @default.
- W2103265200 cites W2041674913 @default.
- W2103265200 cites W2043203820 @default.
- W2103265200 cites W2049137252 @default.
- W2103265200 cites W2050249780 @default.
- W2103265200 cites W2050812367 @default.
- W2103265200 cites W2052738269 @default.
- W2103265200 cites W2055129041 @default.
- W2103265200 cites W2059592231 @default.
- W2103265200 cites W2059968749 @default.
- W2103265200 cites W2067455706 @default.
- W2103265200 cites W2071816185 @default.
- W2103265200 cites W2089417246 @default.
- W2103265200 cites W2089430148 @default.
- W2103265200 cites W2096172906 @default.
- W2103265200 cites W2099343472 @default.
- W2103265200 cites W2101177674 @default.
- W2103265200 cites W2108741433 @default.
- W2103265200 cites W2109736972 @default.
- W2103265200 cites W2112253421 @default.
- W2103265200 cites W2114040881 @default.
- W2103265200 cites W2115779097 @default.
- W2103265200 cites W2122277359 @default.
- W2103265200 cites W2123883670 @default.
- W2103265200 cites W2124015466 @default.
- W2103265200 cites W2131116802 @default.
- W2103265200 cites W2151562469 @default.
- W2103265200 cites W2154595259 @default.
- W2103265200 cites W2155634448 @default.
- W2103265200 cites W2157099671 @default.
- W2103265200 cites W2160704282 @default.
- W2103265200 cites W2161584400 @default.
- W2103265200 cites W2163432887 @default.
- W2103265200 cites W2164773184 @default.
- W2103265200 cites W2168819459 @default.
- W2103265200 cites W2171013411 @default.
- W2103265200 cites W4378718562 @default.
- W2103265200 doi "https://doi.org/10.1371/journal.pgen.1002540" @default.
- W2103265200 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3291542" @default.
- W2103265200 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22396659" @default.
- W2103265200 hasPublicationYear "2012" @default.
- W2103265200 type Work @default.
- W2103265200 sameAs 2103265200 @default.
- W2103265200 citedByCount "17" @default.
- W2103265200 countsByYear W21032652002012 @default.
- W2103265200 countsByYear W21032652002013 @default.
- W2103265200 countsByYear W21032652002014 @default.
- W2103265200 countsByYear W21032652002015 @default.