FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103277101> ?p ?o ?g. }

• W2103277101 abstract "Knowing “what” and “where” brings us closer to “how”. In this project, we have used mass spectrometers to know “what” is present inside breast cancer cells and “where” these molecules are located in human breast tumor xenograft models. Understanding the molecular composition of breast tumors is a crucial step towards the development of new anti-cancer treatment strategies. In the present research project we performed experiments in collaboration with a state-of-the-art proteomics facility located in the Netherlands Proteomic Centre in Utrecht, in which we identified and quantified more than 6, 000 different proteins from breast cancer cells. We focused on hypoxia-induced proteome changes and identified hundreds of up- and down-regulated proteins in hypoxic breast cancer cells. The up-regulated proteins belong to cellular pathways responsible for cell survival, energy metabolism, apoptosis, heme metabolism and immune system modulation. Next, we employed the mass spectrometry imaging facility at the FOM-Institute AMOLF in Amsterdam and the ICMIC molecular imaging facility at The Johns Hopkins University School of Medicine, where we combined bright field/fluorescence microscopy, mass spectrometry imaging and histological tissue staining to visualize and explore the molecular composition of breast tumor xenograft tissue. Microscopy provided information about the localization of hypoxic as well as necrotic tumor regions, while mass spectrometry imaged biomolecular distributions in these heterogeneously distributed tumor regions. In order to co-register the images obtained by different imaging modalities we developed a novel fiducial marker system. Fiducial markers such as cresyl violet, Ponceau S, and bromophenol blue possess a combination of optical and molecular properties that resulted in a clear optical and mass spectrometric signature. This system allowed us to precisely overlay multiple 2D images by providing reference points. It also proved to be a powerful tool for 3D volume reconstruction of different breast tumor microenvironments imaged in parallel by bright field, fluorescence, and mass spectrometric imaging. With our 3D MSI technique we were able to visualise biomolecular distributions in different tumor microenvironments, in all three dimensions and with high molecular specificity. One of the major goals of the project was to localize and investigate effects of hypoxia in breast tumors. A genetically modified breast cancer cell line expressing tdTomato red fluorescent protein under hypoxic conditions was used to generate breast tumor xenograft models. Traditionally, fluorescent proteins are imaged by fluorescence microscopy. Here, for the first time we employed mass spectrometry imaging to visualize the distribution of the tdTomato red fluorescent protein directly from breast tumor tissue. While microscopic detection utilizes this protein’s intrinsic feature of fluorescence, MSI detects this protein’s unique amino acid sequence. Multimodal imaging visualized intact phospholipids in viable tumor tissue while a higher concentration of lysolipids was detected in necrotic regions. Hypoxic tumor regions contained different metabolites, lipids and proteins than well oxygenated, normoxic tumor regions. Biomolecules such as acylcarnitines, a number of different phosphatidylcholines and sphingomyelins were mostly detected in hypoxic tumor regions. Multimodal imaging led us to thus far unexplored scientific territories and shed light on processes occurring in different microenvironments inside breast tumors." @default.
• W2103277101 created "2016-06-24" @default.
• W2103277101 creator A5041745947 @default.
• W2103277101 date "2012-11-05" @default.
• W2103277101 modified "2023-09-28" @default.
• W2103277101 title "Multimodal imaging of hypoxia in breast cancer" @default.
• W2103277101 hasPublicationYear "2012" @default.
• W2103277101 type Work @default.
• W2103277101 sameAs 2103277101 @default.
• W2103277101 citedByCount "0" @default.
• W2103277101 crossrefType "dissertation" @default.
• W2103277101 hasAuthorship W2103277101A5041745947 @default.
• W2103277101 hasConcept C104317684 @default.
• W2103277101 hasConcept C104397665 @default.
• W2103277101 hasConcept C121608353 @default.
• W2103277101 hasConcept C126322002 @default.
• W2103277101 hasConcept C126838900 @default.
• W2103277101 hasConcept C136339569 @default.
• W2103277101 hasConcept C142724271 @default.
• W2103277101 hasConcept C150903083 @default.
• W2103277101 hasConcept C162356407 @default.
• W2103277101 hasConcept C173974348 @default.
• W2103277101 hasConcept C185592680 @default.
• W2103277101 hasConcept C207001950 @default.
• W2103277101 hasConcept C24066741 @default.
• W2103277101 hasConcept C43617362 @default.
• W2103277101 hasConcept C46111723 @default.
• W2103277101 hasConcept C502942594 @default.
• W2103277101 hasConcept C530470458 @default.
• W2103277101 hasConcept C55493867 @default.
• W2103277101 hasConcept C60644358 @default.
• W2103277101 hasConcept C71924100 @default.
• W2103277101 hasConcept C86803240 @default.
• W2103277101 hasConceptScore W2103277101C104317684 @default.
• W2103277101 hasConceptScore W2103277101C104397665 @default.
• W2103277101 hasConceptScore W2103277101C121608353 @default.
• W2103277101 hasConceptScore W2103277101C126322002 @default.
• W2103277101 hasConceptScore W2103277101C126838900 @default.
• W2103277101 hasConceptScore W2103277101C136339569 @default.
• W2103277101 hasConceptScore W2103277101C142724271 @default.
• W2103277101 hasConceptScore W2103277101C150903083 @default.
• W2103277101 hasConceptScore W2103277101C162356407 @default.
• W2103277101 hasConceptScore W2103277101C173974348 @default.
• W2103277101 hasConceptScore W2103277101C185592680 @default.
• W2103277101 hasConceptScore W2103277101C207001950 @default.
• W2103277101 hasConceptScore W2103277101C24066741 @default.
• W2103277101 hasConceptScore W2103277101C43617362 @default.
• W2103277101 hasConceptScore W2103277101C46111723 @default.
• W2103277101 hasConceptScore W2103277101C502942594 @default.
• W2103277101 hasConceptScore W2103277101C530470458 @default.
• W2103277101 hasConceptScore W2103277101C55493867 @default.
• W2103277101 hasConceptScore W2103277101C60644358 @default.
• W2103277101 hasConceptScore W2103277101C71924100 @default.
• W2103277101 hasConceptScore W2103277101C86803240 @default.
• W2103277101 hasLocation W21032771011 @default.
• W2103277101 hasOpenAccess W2103277101 @default.
• W2103277101 hasPrimaryLocation W21032771011 @default.
• W2103277101 hasRelatedWork W1486776188 @default.
• W2103277101 hasRelatedWork W1966821858 @default.
• W2103277101 hasRelatedWork W1984259255 @default.
• W2103277101 hasRelatedWork W1992303404 @default.
• W2103277101 hasRelatedWork W2008288591 @default.
• W2103277101 hasRelatedWork W2037492592 @default.
• W2103277101 hasRelatedWork W2075363723 @default.
• W2103277101 hasRelatedWork W2147862016 @default.
• W2103277101 hasRelatedWork W2199795028 @default.
• W2103277101 hasRelatedWork W2233826063 @default.
• W2103277101 hasRelatedWork W2295391911 @default.
• W2103277101 hasRelatedWork W2400075662 @default.
• W2103277101 hasRelatedWork W2406448959 @default.
• W2103277101 hasRelatedWork W2755727898 @default.
• W2103277101 hasRelatedWork W2765970907 @default.
• W2103277101 hasRelatedWork W2889450654 @default.
• W2103277101 hasRelatedWork W304935490 @default.
• W2103277101 hasRelatedWork W3207752431 @default.
• W2103277101 hasRelatedWork W332396864 @default.
• W2103277101 hasRelatedWork W56243216 @default.
• W2103277101 isParatext "false" @default.
• W2103277101 isRetracted "false" @default.
• W2103277101 magId "2103277101" @default.
• W2103277101 workType "dissertation" @default.