FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103292231> ?p ?o ?g. }

• W2103292231 abstract "Abstract Background Channel proteins like FhuA can be an alternative to artificial chemically synthesized nanopores. To reach such goals, channel proteins must be flexible enough to be modified in their geometry, i.e . length and diameter. As continuation of a previous study in which we addressed the lengthening of the channel, here we report the increasing of the channel diameter by genetic engineering. Results The FhuA Δ1-159 diameter increase has been obtained by doubling the amino acid sequence of the first two N-terminal β-strands, resulting in variant FhuA Δ1-159 Exp. The total number of β-strands increased from 22 to 24 and the channel surface area is expected to increase by ~16%. The secondary structure analysis by circular dichroism (CD) spectroscopy shows a high β-sheet content, suggesting the correct folding of FhuA Δ1-159 Exp. To further prove the FhuA Δ1-159 Exp channel functionality, kinetic measurement using the HRP-TMB assay (HRP = Horse Radish Peroxidase, TMB = 3,3',5,5'-tetramethylbenzidine) were conducted. The results indicated a 17% faster diffusion kinetic for FhuA Δ1-159 Exp as compared to FhuA Δ1-159, well correlated to the expected channel surface area increase of ~16%. Conclusion In this study using a simple semi rational approach the FhuA Δ1-159 diameter was enlarged. By combining the actual results with the previous ones on the FhuA Δ1-159 lengthening a new set of synthetic nanochannels with desired lengths and diameters can be produced, broadening the FhuA Δ1-159 applications. As large scale protein production is possible our approach can give a contribution to nanochannel industrial applications." @default.
• W2103292231 created "2016-06-24" @default.
• W2103292231 creator A5025130254 @default.
• W2103292231 creator A5064879255 @default.
• W2103292231 creator A5079186487 @default.
• W2103292231 date "2011-08-19" @default.
• W2103292231 modified "2023-10-11" @default.
• W2103292231 title "Engineering of an E. coli outer membrane protein FhuA with increased channel diameter" @default.
• W2103292231 cites W1528201008 @default.
• W2103292231 cites W163411833 @default.
• W2103292231 cites W1649220013 @default.
• W2103292231 cites W1965104216 @default.
• W2103292231 cites W1972710564 @default.
• W2103292231 cites W1974700625 @default.
• W2103292231 cites W1998620676 @default.
• W2103292231 cites W1999157466 @default.
• W2103292231 cites W2020540263 @default.
• W2103292231 cites W2023174224 @default.
• W2103292231 cites W2027596001 @default.
• W2103292231 cites W2029953061 @default.
• W2103292231 cites W2038634804 @default.
• W2103292231 cites W2038673006 @default.
• W2103292231 cites W2045247642 @default.
• W2103292231 cites W2064262345 @default.
• W2103292231 cites W2073415221 @default.
• W2103292231 cites W2076166680 @default.
• W2103292231 cites W2078406332 @default.
• W2103292231 cites W2084596253 @default.
• W2103292231 cites W2084608838 @default.
• W2103292231 cites W2096736704 @default.
• W2103292231 cites W2100837269 @default.
• W2103292231 cites W2103433377 @default.
• W2103292231 cites W2117717931 @default.
• W2103292231 cites W2119864595 @default.
• W2103292231 cites W2127177253 @default.
• W2103292231 cites W2138915457 @default.
• W2103292231 cites W2139385860 @default.
• W2103292231 cites W2141375922 @default.
• W2103292231 cites W2149076892 @default.
• W2103292231 doi "https://doi.org/10.1186/1477-3155-9-33" @default.
• W2103292231 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3170585" @default.
• W2103292231 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21854627" @default.
• W2103292231 hasPublicationYear "2011" @default.
• W2103292231 type Work @default.
• W2103292231 sameAs 2103292231 @default.
• W2103292231 citedByCount "26" @default.
• W2103292231 countsByYear W21032922312012 @default.
• W2103292231 countsByYear W21032922312013 @default.
• W2103292231 countsByYear W21032922312015 @default.
• W2103292231 countsByYear W21032922312016 @default.
• W2103292231 countsByYear W21032922312017 @default.
• W2103292231 countsByYear W21032922312018 @default.
• W2103292231 countsByYear W21032922312019 @default.
• W2103292231 countsByYear W21032922312020 @default.
• W2103292231 countsByYear W21032922312021 @default.
• W2103292231 countsByYear W21032922312023 @default.
• W2103292231 crossrefType "journal-article" @default.
• W2103292231 hasAuthorship W2103292231A5025130254 @default.
• W2103292231 hasAuthorship W2103292231A5064879255 @default.
• W2103292231 hasAuthorship W2103292231A5079186487 @default.
• W2103292231 hasBestOaLocation W21032922311 @default.
• W2103292231 hasConcept C119473381 @default.
• W2103292231 hasConcept C119599485 @default.
• W2103292231 hasConcept C12554922 @default.
• W2103292231 hasConcept C127413603 @default.
• W2103292231 hasConcept C133571119 @default.
• W2103292231 hasConcept C141795571 @default.
• W2103292231 hasConcept C171250308 @default.
• W2103292231 hasConcept C178790620 @default.
• W2103292231 hasConcept C185592680 @default.
• W2103292231 hasConcept C192562407 @default.
• W2103292231 hasConcept C2776545253 @default.
• W2103292231 hasConcept C32909587 @default.
• W2103292231 hasConcept C8010536 @default.
• W2103292231 hasConcept C86803240 @default.
• W2103292231 hasConceptScore W2103292231C119473381 @default.
• W2103292231 hasConceptScore W2103292231C119599485 @default.
• W2103292231 hasConceptScore W2103292231C12554922 @default.
• W2103292231 hasConceptScore W2103292231C127413603 @default.
• W2103292231 hasConceptScore W2103292231C133571119 @default.
• W2103292231 hasConceptScore W2103292231C141795571 @default.
• W2103292231 hasConceptScore W2103292231C171250308 @default.
• W2103292231 hasConceptScore W2103292231C178790620 @default.
• W2103292231 hasConceptScore W2103292231C185592680 @default.
• W2103292231 hasConceptScore W2103292231C192562407 @default.
• W2103292231 hasConceptScore W2103292231C2776545253 @default.
• W2103292231 hasConceptScore W2103292231C32909587 @default.
• W2103292231 hasConceptScore W2103292231C8010536 @default.
• W2103292231 hasConceptScore W2103292231C86803240 @default.
• W2103292231 hasIssue "1" @default.
• W2103292231 hasLocation W21032922311 @default.
• W2103292231 hasLocation W21032922312 @default.
• W2103292231 hasLocation W21032922313 @default.
• W2103292231 hasLocation W21032922314 @default.
• W2103292231 hasLocation W21032922315 @default.
• W2103292231 hasLocation W21032922316 @default.
• W2103292231 hasOpenAccess W2103292231 @default.
• W2103292231 hasPrimaryLocation W21032922311 @default.
• W2103292231 hasRelatedWork W1495621886 @default.
• W2103292231 hasRelatedWork W1515040117 @default.

• next