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- W2103295593 abstract "The peritoneum is a serous membrane consisting of different kinds of cells and extracellular matrix components (ECM). The aim of the present study was to develop a three-dimensional (3D) in vitro culture system for possible investigation of pathological conditions of the peritoneum. Human omental mesothelial cells (MC) and endothelial cells from the umbilical vein (EC) were cultivated either on (MC) or in (EC) a preformed type I collagen matrix. In 3D culture mesothelial cells showed their phenotypical in vivo characteristics and the synthesis of a new basal membrane (BM). Endothelial cells developed vessel-like structures, produce a BM and express E-selectin after TNF-alpha stimulation. This 3D culture system presents extended possibilities for analyzing mesothelial and endothelial cell behavior as well as the cell-cell and cell-matrix interactions involved in several pathological processes in the peritoneum." @default.
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- W2103295593 date "1997-10-01" @default.
- W2103295593 modified "2023-10-02" @default.
- W2103295593 title "Reconstruction of Peritoneal-like Structure in Three-Dimensional Collagen Gel Matrix Culture" @default.
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- W2103295593 doi "https://doi.org/10.1006/excr.1997.3724" @default.
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