Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103302030> ?p ?o ?g. }
- W2103302030 endingPage "2173" @default.
- W2103302030 startingPage "2160" @default.
- W2103302030 abstract "Like animal cells, fission yeast divides by assembling actin filaments into a contractile ring. In addition to formin Cdc12p and profilin, the single tropomyosin isoform SpTm is required for contractile ring assembly. Cdc12p nucleates actin filaments and remains processively associated with the elongating barbed end while driving the addition of profilin-actin. SpTm is thought to stabilize mature filaments, but it is not known how SpTm localizes to the contractile ring and whether SpTm plays a direct role in Cdc12p-mediated actin polymerization. Using “bulk” and single actin filament assays, we discovered that Cdc12p can recruit SpTm to actin filaments and that SpTm has diverse effects on Cdc12p-mediated actin assembly. On its own, SpTm inhibits actin filament elongation and depolymerization. However, Cdc12p completely overcomes the combined inhibition of actin nucleation and barbed end elongation by profilin and SpTm. Furthermore, SpTm increases the length of Cdc12p-nucleated actin filaments by enhancing the elongation rate twofold and by allowing them to anneal end to end. In contrast, SpTm ultimately turns off Cdc12p-mediated elongation by “trapping” Cdc12p within annealed filaments or by dissociating Cdc12p from the barbed end. Therefore, SpTm makes multiple contributions to contractile ring assembly during and after actin polymerization." @default.
- W2103302030 created "2016-06-24" @default.
- W2103302030 creator A5023734061 @default.
- W2103302030 creator A5073460174 @default.
- W2103302030 creator A5077776525 @default.
- W2103302030 date "2009-04-15" @default.
- W2103302030 modified "2023-09-23" @default.
- W2103302030 title "Role of Tropomyosin in Formin-mediated Contractile Ring Assembly in Fission Yeast" @default.
- W2103302030 cites W1523872131 @default.
- W2103302030 cites W1595495768 @default.
- W2103302030 cites W1964617500 @default.
- W2103302030 cites W1965117042 @default.
- W2103302030 cites W1968381085 @default.
- W2103302030 cites W1968657392 @default.
- W2103302030 cites W1973028538 @default.
- W2103302030 cites W1974975141 @default.
- W2103302030 cites W1975302750 @default.
- W2103302030 cites W1975499333 @default.
- W2103302030 cites W1977436020 @default.
- W2103302030 cites W1982025705 @default.
- W2103302030 cites W1983539343 @default.
- W2103302030 cites W1984575308 @default.
- W2103302030 cites W1985230755 @default.
- W2103302030 cites W1985481160 @default.
- W2103302030 cites W1987361448 @default.
- W2103302030 cites W1988215817 @default.
- W2103302030 cites W1989133939 @default.
- W2103302030 cites W1990565440 @default.
- W2103302030 cites W1997419253 @default.
- W2103302030 cites W2006784026 @default.
- W2103302030 cites W2007391251 @default.
- W2103302030 cites W2011343310 @default.
- W2103302030 cites W2014983646 @default.
- W2103302030 cites W2019559746 @default.
- W2103302030 cites W2020081859 @default.
- W2103302030 cites W2022492503 @default.
- W2103302030 cites W2024349874 @default.
- W2103302030 cites W2029610828 @default.
- W2103302030 cites W2030768863 @default.
- W2103302030 cites W2034885034 @default.
- W2103302030 cites W2040409993 @default.
- W2103302030 cites W2047853156 @default.
- W2103302030 cites W2049797460 @default.
- W2103302030 cites W2050301961 @default.
- W2103302030 cites W2054302856 @default.
- W2103302030 cites W2056889742 @default.
- W2103302030 cites W2058377873 @default.
- W2103302030 cites W2064071063 @default.
- W2103302030 cites W2073117514 @default.
- W2103302030 cites W2077593465 @default.
- W2103302030 cites W2081875880 @default.
- W2103302030 cites W2082161843 @default.
- W2103302030 cites W2083523423 @default.
- W2103302030 cites W2084453347 @default.
- W2103302030 cites W2085308794 @default.
- W2103302030 cites W2086088782 @default.
- W2103302030 cites W2088635839 @default.
- W2103302030 cites W2091060631 @default.
- W2103302030 cites W2094107289 @default.
- W2103302030 cites W2094269474 @default.
- W2103302030 cites W2094810239 @default.
- W2103302030 cites W2095141865 @default.
- W2103302030 cites W2095649347 @default.
- W2103302030 cites W2102726150 @default.
- W2103302030 cites W2103966138 @default.
- W2103302030 cites W2105981507 @default.
- W2103302030 cites W2106723342 @default.
- W2103302030 cites W2108167818 @default.
- W2103302030 cites W2108678775 @default.
- W2103302030 cites W2113179909 @default.
- W2103302030 cites W2114292341 @default.
- W2103302030 cites W2116344057 @default.
- W2103302030 cites W2117494028 @default.
- W2103302030 cites W2126496496 @default.
- W2103302030 cites W2127303707 @default.
- W2103302030 cites W2127735672 @default.
- W2103302030 cites W2129890878 @default.
- W2103302030 cites W2137213699 @default.
- W2103302030 cites W2140686956 @default.
- W2103302030 cites W2142173778 @default.
- W2103302030 cites W2146663079 @default.
- W2103302030 cites W2147624113 @default.
- W2103302030 cites W2149909679 @default.
- W2103302030 cites W2155653297 @default.
- W2103302030 cites W2156523078 @default.
- W2103302030 cites W2158045056 @default.
- W2103302030 cites W2162225623 @default.
- W2103302030 cites W2164590764 @default.
- W2103302030 cites W2171956751 @default.
- W2103302030 cites W2187601539 @default.
- W2103302030 cites W2194986544 @default.
- W2103302030 cites W2254341277 @default.
- W2103302030 cites W2266663623 @default.
- W2103302030 cites W2419450110 @default.
- W2103302030 doi "https://doi.org/10.1091/mbc.e08-12-1201" @default.
- W2103302030 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2669024" @default.
- W2103302030 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19244341" @default.
- W2103302030 hasPublicationYear "2009" @default.