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• W2103308965 abstract "To differentiate the Borrelia burgdorferi sensu lato genospecies, LightCycler real-time PCR was used for the fluorescence (SYBR Green I) melting curve analysis of borrelial recA gene PCR products. The specific melting temperature analyzed is a function of the GC/AT ratio, length, and nucleotide sequence of the amplified product. A total of 32 DNA samples were tested. Of them three were isolated from B. burgdorferi reference strains and 16 were isolated from B. burgdorferi strains cultured from Ixodes ricinus ticks; 13 were directly isolated from nine human biopsy specimens and four I. ricinus tick midguts. The melting temperature of B. garinii was 2 degrees C lower than that of B. burgdorferi sensu stricto and B. afzelii. Melting curve analysis offers a rapid alternative for identification and detection of B. burgdorferi sensu lato genospecies." @default.
• W2103308965 created "2016-06-24" @default.
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• W2103308965 date "2000-07-01" @default.
• W2103308965 modified "2023-10-16" @default.
• W2103308965 title "Rapid Differentiation of <i>Borrelia garinii</i> from <i>Borrelia afzelii</i> and <i>Borrelia burgdorferi</i> Sensu Stricto by LightCycler Fluorescence Melting Curve Analysis of a PCR Product of the <i>recA</i> Gene" @default.
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• W2103308965 doi "https://doi.org/10.1128/jcm.38.7.2756-2759.2000" @default.
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