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- W2103362487 abstract "Aleutian mink disease parvovirus (ADV), causes an immune disorder with a persistent infection of lymphoid organs in adult mink. We studied replication of ADV in gel-supported histocultures prepared from adult mink mesenteric lymph node (MLN). Evidence of virus replication in the histocultures was first observed by indirect immunofluorescence 72 h after incubation with virus. Cells resembling lymphocytes and macrophages contained both ADV capsid (VP2) and nonstructural (NS1 and NS2) proteins, and were present in a distribution suggestive of infected cells within germinal centres. ADV replicative form and encapsidated virion DNA were also detected in infected histocultures at time-points after 72 h. In addition, we were able to passage ADV-Utah to a new round of histocultures. These results suggested that the infected cells were actual target cells for ADV replication and that productive ADV-Utah replication, complete with the generation of virus, was occurring in the histocultures. The mink MLN histocultures provide a system to study the replication and molecular pathogenesis of ADV in target tissues." @default.
- W2103362487 created "2016-06-24" @default.
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- W2103362487 date "2000-02-01" @default.
- W2103362487 modified "2023-10-12" @default.
- W2103362487 title "Replication of Aleutian mink disease parvovirus in mink lymph node histocultures" @default.
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- W2103362487 doi "https://doi.org/10.1099/0022-1317-81-2-335" @default.
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