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• W2103368045 abstract "Our phylogenetic analysis of three endemic species of the Australian tiger beetle genus Pseudotetracha (Fleutiaux, 1864) from South Australia used sequences of two fragments of the mitochondrial genes 16S rRNA and cytochrome oxidase III. A matrix for each gene and two combined matrices were constructed. We compared these three riparian species, together with data from nine taxa of this genus available in GenBank, using parsimony and Bayesian methods. These molecular results are in agreement with the phylogenetic hypothesis for the blackburni/murchisona species complex previously proposed based on morphology, whereas other recent molecular analyses have questioned the existence of this species complex. In all of our analyses, samples of P. blackburni divided into two statistically supported clades, one of which is more closely related to P. mendacia and P. pulchra than to the other P. blackburni clade. This suggests the existence of a cryptic new species. Additionally, we analysed chromosomes of the second metaphase cells of members of the two clades. The observations showed different karyotypes as blackburni-1 has two types of second meiotic metaphase cells with 11 and 12 chromosomes, whereas in blackburni-2, all cells have 12 chromosomes, adding evidence for the putative existence of two species. Nuestro análisis de tres especies del género endémico australiano Pseudotetracha Fleutiaux, 1864 recolectadas en Australia del Sur usa secuencias de dos fragmentos de los genes mitocondriales ARNr 16S y citocromo oxidasa III. Fue construida una matriz para cada gen y otras dos matrices combinadas. Comparamos estas tres especies riparias junto con datos de nueve taxones de este género disponibles en la base de datos GenBank, por medio de métodos de parsimonia y bayesianos. Los resultados moleculares se muestran de acuerdo con la hipótesis filogenética acerca del complejo de especies blackburni/murchisona propuesto previamente en base a la morfología, mientras que otros estudios moleculares más recientes han cuestionado la existencia de este complejo de especies. En todos los análisis, las muestras de P. blackburni se dividen en dos clados con soporte estadístico, separados por las secuencias de P. mendacia y P. pulchra, hecho que sugiere la existencia de una nueva especie críptica. Adicionalmente se han analizado cromosomas en células de la segunda división de la meiosis en miembros de ambos clados. Las observaciones mostraron cariotipos diferentes, así blackburni-1 presentó dos tipos de células en la segunda división meiótica con 11 y 12 cromosomas respectivamente, mientras que en blackburni-2 todas las células mostraron 12 cromosomas, proporcionando así evidencias adicionales que apoyan la posible existencia de dos especies. Table S1. List of samples used in this work, showing the collecting data and which sequences were obtained from each sample. Table S2. Species delimitation results above the Bayesian inference trees. Only species with more than one sample available are shown. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article." @default.
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• W2103368045 date "2012-02-24" @default.
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• W2103368045 title "The blackburni/murchisona species complex in Australian Pseudotetracha (Coleoptera: Carabidae: Cicindelinae: Megacephalini): evaluating molecular and karyological evidence" @default.
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• W2103368045 doi "https://doi.org/10.1111/j.1439-0469.2012.00659.x" @default.
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