Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103379050> ?p ?o ?g. }
- W2103379050 abstract "We purified saponin hydrolases from Aspergillus oryzae PF1224 and Eupenicillium brefeldianum PF1226. It was confirmed that the enzymes from A. oryzae PF1224 (Sda1) and E. brefeldianum PF1226 (Sde1) are glycoproteins with molecular masses of 82 and 90 kDa respectively. The deduced amino acid sequences of each enzyme from the cloned genes (sda1 or sde1) showed approximately 50% homology with that of the saponin hydrolase Sdn1 from Neocosmospora vasinfecta var. vasinfecta PF1225 (DDBJ accession no. AB110615). When sda1 and sde1 were expressed in the host Trichoderma viride under the control of the cellobiohydrolase I gene promoter, recombinant proteins were secreted with molecular masses of 77 and 67 kDa respectively. These recombinant enzymes hydrolyzed soyasaponin I to soyasapogenol B and triose, and its substrate specificities for glycosides were similar to that of Sdn1, but the specific activities of these enzymes were lower than that of Sdn1." @default.
- W2103379050 created "2016-06-24" @default.
- W2103379050 creator A5043387970 @default.
- W2103379050 creator A5055074107 @default.
- W2103379050 creator A5059436955 @default.
- W2103379050 creator A5076006402 @default.
- W2103379050 date "2005-01-01" @default.
- W2103379050 modified "2023-09-25" @default.
- W2103379050 title "Cloning and Characterization of Saponin Hydrolases fromAspergillus oryzaeandEupenicillium brefeldianum" @default.
- W2103379050 cites W1763383785 @default.
- W2103379050 cites W1764658750 @default.
- W2103379050 cites W1964158438 @default.
- W2103379050 cites W1964307727 @default.
- W2103379050 cites W1966758367 @default.
- W2103379050 cites W19682104 @default.
- W2103379050 cites W1971578535 @default.
- W2103379050 cites W1983107329 @default.
- W2103379050 cites W1992017511 @default.
- W2103379050 cites W1996535761 @default.
- W2103379050 cites W2002275634 @default.
- W2103379050 cites W2003435396 @default.
- W2103379050 cites W2009171157 @default.
- W2103379050 cites W2012537709 @default.
- W2103379050 cites W2018095902 @default.
- W2103379050 cites W2027869349 @default.
- W2103379050 cites W2030794316 @default.
- W2103379050 cites W2041105433 @default.
- W2103379050 cites W2046223523 @default.
- W2103379050 cites W2050687766 @default.
- W2103379050 cites W2058353118 @default.
- W2103379050 cites W2060531928 @default.
- W2103379050 cites W2072020101 @default.
- W2103379050 cites W2072836964 @default.
- W2103379050 cites W2075120508 @default.
- W2103379050 cites W2099834800 @default.
- W2103379050 cites W2103231201 @default.
- W2103379050 cites W2117512575 @default.
- W2103379050 cites W2122524572 @default.
- W2103379050 cites W2139552251 @default.
- W2103379050 cites W2149800654 @default.
- W2103379050 cites W2312693965 @default.
- W2103379050 cites W2335694731 @default.
- W2103379050 cites W251520332 @default.
- W2103379050 cites W4231758916 @default.
- W2103379050 cites W4245858708 @default.
- W2103379050 cites W73249300 @default.
- W2103379050 doi "https://doi.org/10.1271/bbb.69.2178" @default.
- W2103379050 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16306700" @default.
- W2103379050 hasPublicationYear "2005" @default.
- W2103379050 type Work @default.
- W2103379050 sameAs 2103379050 @default.
- W2103379050 citedByCount "5" @default.
- W2103379050 countsByYear W21033790502018 @default.
- W2103379050 crossrefType "journal-article" @default.
- W2103379050 hasAuthorship W2103379050A5043387970 @default.
- W2103379050 hasAuthorship W2103379050A5055074107 @default.
- W2103379050 hasAuthorship W2103379050A5059436955 @default.
- W2103379050 hasAuthorship W2103379050A5076006402 @default.
- W2103379050 hasConcept C104317684 @default.
- W2103379050 hasConcept C108625454 @default.
- W2103379050 hasConcept C141603559 @default.
- W2103379050 hasConcept C142724271 @default.
- W2103379050 hasConcept C153911025 @default.
- W2103379050 hasConcept C167625842 @default.
- W2103379050 hasConcept C181199279 @default.
- W2103379050 hasConcept C196032511 @default.
- W2103379050 hasConcept C19924922 @default.
- W2103379050 hasConcept C204787440 @default.
- W2103379050 hasConcept C2776125036 @default.
- W2103379050 hasConcept C2778564307 @default.
- W2103379050 hasConcept C2780052172 @default.
- W2103379050 hasConcept C2781204770 @default.
- W2103379050 hasConcept C40767141 @default.
- W2103379050 hasConcept C55493867 @default.
- W2103379050 hasConcept C59822182 @default.
- W2103379050 hasConcept C71924100 @default.
- W2103379050 hasConcept C86803240 @default.
- W2103379050 hasConceptScore W2103379050C104317684 @default.
- W2103379050 hasConceptScore W2103379050C108625454 @default.
- W2103379050 hasConceptScore W2103379050C141603559 @default.
- W2103379050 hasConceptScore W2103379050C142724271 @default.
- W2103379050 hasConceptScore W2103379050C153911025 @default.
- W2103379050 hasConceptScore W2103379050C167625842 @default.
- W2103379050 hasConceptScore W2103379050C181199279 @default.
- W2103379050 hasConceptScore W2103379050C196032511 @default.
- W2103379050 hasConceptScore W2103379050C19924922 @default.
- W2103379050 hasConceptScore W2103379050C204787440 @default.
- W2103379050 hasConceptScore W2103379050C2776125036 @default.
- W2103379050 hasConceptScore W2103379050C2778564307 @default.
- W2103379050 hasConceptScore W2103379050C2780052172 @default.
- W2103379050 hasConceptScore W2103379050C2781204770 @default.
- W2103379050 hasConceptScore W2103379050C40767141 @default.
- W2103379050 hasConceptScore W2103379050C55493867 @default.
- W2103379050 hasConceptScore W2103379050C59822182 @default.
- W2103379050 hasConceptScore W2103379050C71924100 @default.
- W2103379050 hasConceptScore W2103379050C86803240 @default.
- W2103379050 hasLocation W21033790501 @default.
- W2103379050 hasLocation W21033790502 @default.
- W2103379050 hasOpenAccess W2103379050 @default.
- W2103379050 hasPrimaryLocation W21033790501 @default.