FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103385286> ?p ?o ?g. }

• W2103385286 endingPage "1261" @default.
• W2103385286 startingPage "1257" @default.
• W2103385286 abstract "ABSTRACT We designed a method by which to generate antibiotic-resistant strains of Streptococcus pneumoniae at frequencies 4 orders of magnitude greater than the spontaneous mutation rate. The method is based on the natural ability of this organism to be genetically transformed with PCR products carrying sequences homologous to its chromosome. The genes encoding the targets of ciprofloxacin ( parC , encoding the ParC subunit of DNA topoisomerase IV), rifampin ( rpoB , encoding the β subunit of RNA polymerase), and streptomycin ( rpsL , encoding the S12 ribosomal protein) from susceptible laboratory strain R6 were amplified by PCR and used to transform the same strain. Resistant mutants were obtained with a frequency of 10 −4 to 10 −5 , depending on the fidelity of the DNA polymerase used for PCR amplifications. Ciprofloxacin-resistant mutants, for which the MICs were four-to eightfold higher than that for R6, carried a single mutation of a residue in the quinolone resistance-determining region: S79 (change to A, F, or Y) or D83 (change to N or V). Rifampin-resistant strains, for which the MICs were at least 133-fold higher than that for R6, contained a single mutation within cluster I of rpoB : S482 (change to P), Q486 (change to L), D489 (change to V), or H499 (change to L or Y). Streptomycin-resistant mutants, for which the MICs were at least 64-fold higher than that for R6, carried a mutation at either K56 (change to I, R, or T) or K101 (change to E). PCR products obtained from the mutants were able to transform R6 to resistance with high efficiency (>10 4 ). This method could be used to efficiently obtain resistant mutants for any drug whose target is known." @default.
• W2103385286 created "2016-06-24" @default.
• W2103385286 creator A5046206757 @default.
• W2103385286 creator A5059354705 @default.
• W2103385286 date "2003-04-01" @default.
• W2103385286 modified "2023-09-29" @default.
• W2103385286 title "High-Efficiency Generation of Antibiotic-Resistant Strains of <i>Streptococcus pneumoniae</i> by PCR and Transformation" @default.
• W2103385286 cites W1517610680 @default.
• W2103385286 cites W1529428852 @default.
• W2103385286 cites W1557408031 @default.
• W2103385286 cites W1557661275 @default.
• W2103385286 cites W1568741842 @default.
• W2103385286 cites W1571594650 @default.
• W2103385286 cites W1630678156 @default.
• W2103385286 cites W1870121944 @default.
• W2103385286 cites W1967386006 @default.
• W2103385286 cites W1998083475 @default.
• W2103385286 cites W2010704689 @default.
• W2103385286 cites W2015290115 @default.
• W2103385286 cites W2038211892 @default.
• W2103385286 cites W2043932025 @default.
• W2103385286 cites W2047929024 @default.
• W2103385286 cites W2068148879 @default.
• W2103385286 cites W2074726414 @default.
• W2103385286 cites W2088978016 @default.
• W2103385286 cites W2102439761 @default.
• W2103385286 cites W2103268923 @default.
• W2103385286 cites W2105476980 @default.
• W2103385286 cites W2106291449 @default.
• W2103385286 cites W2107131874 @default.
• W2103385286 cites W2107870109 @default.
• W2103385286 cites W2108281648 @default.
• W2103385286 cites W2108360478 @default.
• W2103385286 cites W2122465910 @default.
• W2103385286 cites W2130263042 @default.
• W2103385286 cites W2140725652 @default.
• W2103385286 cites W2146277354 @default.
• W2103385286 cites W2146763898 @default.
• W2103385286 cites W2147990519 @default.
• W2103385286 cites W2168067188 @default.
• W2103385286 cites W2318532494 @default.
• W2103385286 cites W2331102377 @default.
• W2103385286 cites W2336287850 @default.
• W2103385286 cites W3027545634 @default.
• W2103385286 doi "https://doi.org/10.1128/aac.47.4.1257-1261.2003" @default.
• W2103385286 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/152537" @default.
• W2103385286 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12654655" @default.
• W2103385286 hasPublicationYear "2003" @default.
• W2103385286 type Work @default.
• W2103385286 sameAs 2103385286 @default.
• W2103385286 citedByCount "24" @default.
• W2103385286 countsByYear W21033852862014 @default.
• W2103385286 countsByYear W21033852862016 @default.
• W2103385286 countsByYear W21033852862017 @default.
• W2103385286 countsByYear W21033852862018 @default.
• W2103385286 countsByYear W21033852862019 @default.
• W2103385286 countsByYear W21033852862020 @default.
• W2103385286 countsByYear W21033852862021 @default.
• W2103385286 crossrefType "journal-article" @default.
• W2103385286 hasAuthorship W2103385286A5046206757 @default.
• W2103385286 hasAuthorship W2103385286A5059354705 @default.
• W2103385286 hasBestOaLocation W21033852862 @default.
• W2103385286 hasConcept C104317684 @default.
• W2103385286 hasConcept C143065580 @default.
• W2103385286 hasConcept C153911025 @default.
• W2103385286 hasConcept C167125095 @default.
• W2103385286 hasConcept C204241405 @default.
• W2103385286 hasConcept C2778340795 @default.
• W2103385286 hasConcept C2781253189 @default.
• W2103385286 hasConcept C42062724 @default.
• W2103385286 hasConcept C501593827 @default.
• W2103385286 hasConcept C54355233 @default.
• W2103385286 hasConcept C547475151 @default.
• W2103385286 hasConcept C552990157 @default.
• W2103385286 hasConcept C82381507 @default.
• W2103385286 hasConcept C86803240 @default.
• W2103385286 hasConcept C89423630 @default.
• W2103385286 hasConceptScore W2103385286C104317684 @default.
• W2103385286 hasConceptScore W2103385286C143065580 @default.
• W2103385286 hasConceptScore W2103385286C153911025 @default.
• W2103385286 hasConceptScore W2103385286C167125095 @default.
• W2103385286 hasConceptScore W2103385286C204241405 @default.
• W2103385286 hasConceptScore W2103385286C2778340795 @default.
• W2103385286 hasConceptScore W2103385286C2781253189 @default.
• W2103385286 hasConceptScore W2103385286C42062724 @default.
• W2103385286 hasConceptScore W2103385286C501593827 @default.
• W2103385286 hasConceptScore W2103385286C54355233 @default.
• W2103385286 hasConceptScore W2103385286C547475151 @default.
• W2103385286 hasConceptScore W2103385286C552990157 @default.
• W2103385286 hasConceptScore W2103385286C82381507 @default.
• W2103385286 hasConceptScore W2103385286C86803240 @default.
• W2103385286 hasConceptScore W2103385286C89423630 @default.
• W2103385286 hasIssue "4" @default.
• W2103385286 hasLocation W21033852861 @default.
• W2103385286 hasLocation W21033852862 @default.
• W2103385286 hasLocation W21033852863 @default.
• W2103385286 hasLocation W21033852864 @default.
• W2103385286 hasOpenAccess W2103385286 @default.

• next