FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103445583> ?p ?o ?g. }

• W2103445583 endingPage "3456" @default.
• W2103445583 startingPage "3443" @default.
• W2103445583 abstract "ABSTRACT We report the isolation of a Coccidioides immitis gene ( SOWgp ) which encodes an immunodominant, spherule outer wall glycoprotein that is presented as a component of a parasitic phase-specific, membranous layer at the cell surface. The open reading frame of the gene from C. immitis isolate C735 translates a 422-amino-acid (aa) polypeptide that contains 6 copies of a 41- to 47-residue tandem repeat enriched in proline (20.4 mol%) and aspartate (19.7%). Two additional isolates of C. immitis produce SOWgps of different molecular sizes (328 and 375 aa) and show a corresponding difference in the number of tandem repeats (four and five, respectively). The accurate molecular sizes of these proline-rich antigens, as determined by surface-enhanced laser desorption/ionization mass spectrometry, are comparable to the predicted sizes from the translated protein sequences rather than the estimated sizes based on gel-electrophoretic separation. The results of Northern hybridization confirmed that SOWgp expression is parasitic phase specific, and immunoblot studies showed that elevated levels of production of this antigen occurred during early spherule development. The recombinant polypeptide (rSOWp) was shown to bind to mammalian extracellular matrix (ECM) proteins in an in vitro assay (laminin > fibronectin > collagen type IV), suggesting that the parasitic cell surface antigen may function as an adhesin. Deletion of the SOWgp gene by using a targeted gene replacement strategy resulted in partial loss of the ability of intact spherules to bind to ECM proteins and a significant reduction in virulence of the mutant strain. The wild-type gene was restored in the mutant by homologous recombination, and the revertant strain was shown to be as virulent as the parental isolate in our murine model of coccidioidomycosis. The parasitic cell surface glycoprotein encoded by the SOWgp gene appears to function as an adhesin and contributes to the virulence of C. immitis." @default.
• W2103445583 created "2016-06-24" @default.
• W2103445583 creator A5003041501 @default.
• W2103445583 creator A5005636196 @default.
• W2103445583 creator A5016061371 @default.
• W2103445583 creator A5037108900 @default.
• W2103445583 creator A5050645487 @default.
• W2103445583 date "2002-07-01" @default.
• W2103445583 modified "2023-09-26" @default.
• W2103445583 title "A Parasitic Phase-Specific Adhesin of <i>Coccidioides immitis</i> Contributes to the Virulence of This Respiratory Fungal Pathogen" @default.
• W2103445583 cites W138271398 @default.
• W2103445583 cites W1843397982 @default.
• W2103445583 cites W1861595365 @default.
• W2103445583 cites W1918267335 @default.
• W2103445583 cites W1956423653 @default.
• W2103445583 cites W196272473 @default.
• W2103445583 cites W1975304761 @default.
• W2103445583 cites W1994326152 @default.
• W2103445583 cites W1994704939 @default.
• W2103445583 cites W1998221992 @default.
• W2103445583 cites W2006787162 @default.
• W2103445583 cites W2024598696 @default.
• W2103445583 cites W2036858990 @default.
• W2103445583 cites W2051255331 @default.
• W2103445583 cites W2075452661 @default.
• W2103445583 cites W2075848560 @default.
• W2103445583 cites W2079698024 @default.
• W2103445583 cites W2091316728 @default.
• W2103445583 cites W2094031081 @default.
• W2103445583 cites W2099992860 @default.
• W2103445583 cites W2105170573 @default.
• W2103445583 cites W2111240961 @default.
• W2103445583 cites W2114749809 @default.
• W2103445583 cites W2119033485 @default.
• W2103445583 cites W2119140026 @default.
• W2103445583 cites W2120636265 @default.
• W2103445583 cites W2132572684 @default.
• W2103445583 cites W2134023938 @default.
• W2103445583 cites W2136939104 @default.
• W2103445583 cites W2147898421 @default.
• W2103445583 cites W2153531664 @default.
• W2103445583 cites W2156486288 @default.
• W2103445583 cites W2158046215 @default.
• W2103445583 cites W2158714788 @default.
• W2103445583 cites W2160747036 @default.
• W2103445583 cites W2161252280 @default.
• W2103445583 cites W2211776847 @default.
• W2103445583 cites W2916193864 @default.
• W2103445583 cites W4298224778 @default.
• W2103445583 doi "https://doi.org/10.1128/iai.70.7.3443-3456.2002" @default.
• W2103445583 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/128074" @default.
• W2103445583 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12065484" @default.
• W2103445583 hasPublicationYear "2002" @default.
• W2103445583 type Work @default.
• W2103445583 sameAs 2103445583 @default.
• W2103445583 citedByCount "122" @default.
• W2103445583 countsByYear W21034455832012 @default.
• W2103445583 countsByYear W21034455832013 @default.
• W2103445583 countsByYear W21034455832014 @default.
• W2103445583 countsByYear W21034455832015 @default.
• W2103445583 countsByYear W21034455832016 @default.
• W2103445583 countsByYear W21034455832017 @default.
• W2103445583 countsByYear W21034455832018 @default.
• W2103445583 countsByYear W21034455832019 @default.
• W2103445583 countsByYear W21034455832020 @default.
• W2103445583 countsByYear W21034455832021 @default.
• W2103445583 countsByYear W21034455832022 @default.
• W2103445583 countsByYear W21034455832023 @default.
• W2103445583 crossrefType "journal-article" @default.
• W2103445583 hasAuthorship W2103445583A5003041501 @default.
• W2103445583 hasAuthorship W2103445583A5005636196 @default.
• W2103445583 hasAuthorship W2103445583A5016061371 @default.
• W2103445583 hasAuthorship W2103445583A5037108900 @default.
• W2103445583 hasAuthorship W2103445583A5050645487 @default.
• W2103445583 hasBestOaLocation W21034455831 @default.
• W2103445583 hasConcept C104317684 @default.
• W2103445583 hasConcept C108625454 @default.
• W2103445583 hasConcept C141231307 @default.
• W2103445583 hasConcept C147483822 @default.
• W2103445583 hasConcept C153911025 @default.
• W2103445583 hasConcept C179470777 @default.
• W2103445583 hasConcept C27149982 @default.
• W2103445583 hasConcept C2778439347 @default.
• W2103445583 hasConcept C2781030883 @default.
• W2103445583 hasConcept C54355233 @default.
• W2103445583 hasConcept C55493867 @default.
• W2103445583 hasConcept C60987743 @default.
• W2103445583 hasConcept C86803240 @default.
• W2103445583 hasConcept C89423630 @default.
• W2103445583 hasConceptScore W2103445583C104317684 @default.
• W2103445583 hasConceptScore W2103445583C108625454 @default.
• W2103445583 hasConceptScore W2103445583C141231307 @default.
• W2103445583 hasConceptScore W2103445583C147483822 @default.
• W2103445583 hasConceptScore W2103445583C153911025 @default.
• W2103445583 hasConceptScore W2103445583C179470777 @default.
• W2103445583 hasConceptScore W2103445583C27149982 @default.
• W2103445583 hasConceptScore W2103445583C2778439347 @default.
• W2103445583 hasConceptScore W2103445583C2781030883 @default.

• next