FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103478108> ?p ?o ?g. }

• W2103478108 endingPage "486" @default.
• W2103478108 startingPage "473" @default.
• W2103478108 abstract "We report that embryonic stem cells efficiently undergo differentiation in vitro to mesoderm and hematopoietic cells and that this in vitro system recapitulates days 6.5 to 7.5 of mouse hematopoietic development. Embryonic stem cells differentiated as embryoid bodies (EBs) develop erythroid precursors by day 4 of differentiation, and by day 6, more than 85% of EBs contain such cells. A comparative reverse transcriptase-mediated polymerase chain reaction profile of marker genes for primitive endoderm (collagen alpha IV) and mesoderm (Brachyury) indicates that both cell types are present in the developing EBs as well in normal embryos prior to the onset of hematopoiesis. GATA-1, GATA-3, and vav are expressed in both the EBs and embryos just prior to and/or during the early onset of hematopoiesis, indicating that they could play a role in the early stages of hematopoietic development both in vivo and in vitro. The initial stages of hematopoietic development within the EBs occur in the absence of added growth factors and are not significantly influenced by the addition of a broad spectrum of factors, including interleukin-3 (IL-3), IL-1, IL-6, IL-11, erythropoietin, and Kit ligand. At days 10 and 14 of differentiation, EB hematopoiesis is significantly enhanced by the addition of both Kit ligand and IL-11 to the cultures. Kinetic analysis indicates that hematopoietic precursors develop within the EBs in an ordered pattern. Precursors of the primitive erythroid lineage appear first, approximately 24 h before precursors of the macrophage and definitive erythroid lineages. Bipotential neutrophil/macrophage and multilineage precursors appear next, and precursors of the mast cell lineage develop last. The kinetics of precursor development, as well as the growth factor responsiveness of these early cells, is similar to that found in the yolk sac and early fetal liver, indicating that the onset of hematopoiesis within the EBs parallels that found in the embryo." @default.
• W2103478108 created "2016-06-24" @default.
• W2103478108 creator A5000745950 @default.
• W2103478108 creator A5030005993 @default.
• W2103478108 creator A5036956529 @default.
• W2103478108 creator A5063440051 @default.
• W2103478108 date "1993-01-01" @default.
• W2103478108 modified "2023-10-11" @default.
• W2103478108 title "Hematopoietic commitment during embryonic stem cell differentiation in culture." @default.
• W2103478108 cites W121036004 @default.
• W2103478108 cites W135518686 @default.
• W2103478108 cites W1487887046 @default.
• W2103478108 cites W1521285578 @default.
• W2103478108 cites W1526272838 @default.
• W2103478108 cites W1582172850 @default.
• W2103478108 cites W1654720320 @default.
• W2103478108 cites W1925667723 @default.
• W2103478108 cites W1927532086 @default.
• W2103478108 cites W1928245765 @default.
• W2103478108 cites W1946088674 @default.
• W2103478108 cites W1948275303 @default.
• W2103478108 cites W1971760578 @default.
• W2103478108 cites W1972736348 @default.
• W2103478108 cites W1980482182 @default.
• W2103478108 cites W1984020249 @default.
• W2103478108 cites W1987933233 @default.
• W2103478108 cites W1992160731 @default.
• W2103478108 cites W1992704301 @default.
• W2103478108 cites W1997257161 @default.
• W2103478108 cites W1998118848 @default.
• W2103478108 cites W1998606350 @default.
• W2103478108 cites W2000602253 @default.
• W2103478108 cites W2013100111 @default.
• W2103478108 cites W2018855387 @default.
• W2103478108 cites W2020782522 @default.
• W2103478108 cites W2022851764 @default.
• W2103478108 cites W2027802649 @default.
• W2103478108 cites W2037010084 @default.
• W2103478108 cites W2037830205 @default.
• W2103478108 cites W2044569441 @default.
• W2103478108 cites W2044918998 @default.
• W2103478108 cites W2053702504 @default.
• W2103478108 cites W2055568103 @default.
• W2103478108 cites W2068133110 @default.
• W2103478108 cites W2069449601 @default.
• W2103478108 cites W2070273393 @default.
• W2103478108 cites W2071311716 @default.
• W2103478108 cites W2074196850 @default.
• W2103478108 cites W2081328681 @default.
• W2103478108 cites W2084424583 @default.
• W2103478108 cites W2086551569 @default.
• W2103478108 cites W2086981174 @default.
• W2103478108 cites W2089461678 @default.
• W2103478108 cites W2090931042 @default.
• W2103478108 cites W2101710059 @default.
• W2103478108 cites W2104244471 @default.
• W2103478108 cites W2105514165 @default.
• W2103478108 cites W2108490725 @default.
• W2103478108 cites W2110275443 @default.
• W2103478108 cites W2112315202 @default.
• W2103478108 cites W2118024162 @default.
• W2103478108 cites W2119121495 @default.
• W2103478108 cites W2129051084 @default.
• W2103478108 cites W2133148457 @default.
• W2103478108 cites W2134812217 @default.
• W2103478108 cites W2140814882 @default.
• W2103478108 cites W2140922568 @default.
• W2103478108 cites W2160113850 @default.
• W2103478108 cites W2163168560 @default.
• W2103478108 cites W2174542542 @default.
• W2103478108 cites W2180300767 @default.
• W2103478108 cites W2398539765 @default.
• W2103478108 cites W2400189066 @default.
• W2103478108 cites W2403889837 @default.
• W2103478108 cites W2409316477 @default.
• W2103478108 cites W2409560712 @default.
• W2103478108 cites W2413587777 @default.
• W2103478108 cites W2415400904 @default.
• W2103478108 cites W2462635122 @default.
• W2103478108 cites W77719516 @default.
• W2103478108 cites W1605005528 @default.
• W2103478108 doi "https://doi.org/10.1128/mcb.13.1.473" @default.
• W2103478108 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/358927" @default.
• W2103478108 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8417345" @default.
• W2103478108 hasPublicationYear "1993" @default.
• W2103478108 type Work @default.
• W2103478108 sameAs 2103478108 @default.
• W2103478108 citedByCount "787" @default.
• W2103478108 countsByYear W21034781082012 @default.
• W2103478108 countsByYear W21034781082013 @default.
• W2103478108 countsByYear W21034781082014 @default.
• W2103478108 countsByYear W21034781082015 @default.
• W2103478108 countsByYear W21034781082016 @default.
• W2103478108 countsByYear W21034781082017 @default.
• W2103478108 countsByYear W21034781082018 @default.
• W2103478108 countsByYear W21034781082019 @default.
• W2103478108 countsByYear W21034781082020 @default.
• W2103478108 countsByYear W21034781082021 @default.

• next