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• W2103494397 abstract "Abstract Since the application of in vitro techniques to assess the activity of antibiotics against bacteria, there has been an interest in using tissue culture techniques to predict the effects of antineoplastic agents on human cancers. Generally, the in vitro effects have not always correlated with the clinical actions. Recently a refined method for measuring the number of cells in a given tumor capable of being cloned and the effects of cytotoxic agents on the cloned tumor cell growth has been developed. There is now good clinical correlation in patients with multiple myeloma and ovarian carcinomas with the responses to chemotherapeutic agents determined in vitro. The soft agar clonal assay has been used to study the biology of head and neck tumor stem cells. At the University of Virginia, 73 squamous cell carcinomas (SCC) of the head and neck were cloned. The basic characteristics of cloned head and neck tumor cell growth was studied in terms of 1 . single cell origin, 2 . cloning efficiency, 3 . colony development, 4 . linearity, 5 . effects of enzyme disaggregation and 6 . ultrastructural characteristics. The clinical characteristics of T class, N class, stage, survival, and Jakobsson's rating of tumor differentiation were correlated with cloning efficiency to determine if there are predictive survival data. Obtaining single cells from the tumors continues to be a major problem. The use of enzymes (collagenase and DNase) has aided in the production of single cells for culture. The enzymes are superior to mechanical techniques and do not compromise viability or cloning efficiency. Electron microscopy is valuable in documenting the malignant nature of the cloned tumor cells. Of the 73 squamous cell carcinomas that were cultured, 49% demonstrated clonal growth. A number of non‐squamous cell carcinomas were also successfully cloned. The cloning efficiency was variable ranging from .001% to .05%. For the SCC's the median cloning efficiency was .005%. Twenty‐nine SCC's were available for clinical correlations. Chemosensitivity testing was often not feasible because of inadequate numbers of tumor cells in the small specimens and frequent failure of clonal growth. A statistically significant correlation of high cloning efficiency with advanced nodal class, stage, and survival was observed. There was no correlation with T class or Jakobsson's rating. A cloning efficiency of ≤ .005% was associated with a high likelihood of death from disease or the development of recurrent disease. For head and neck squamous cell carcinomas, the determination of cloning efficiency in soft agar appears to be a valuable prognostic indicator." @default.
• W2103494397 created "2016-06-24" @default.
• W2103494397 creator A5051683679 @default.
• W2103494397 date "1982-07-01" @default.
• W2103494397 modified "2023-10-09" @default.
• W2103494397 title "The clonal assay of head and neck tumor cells: Results and clinical correlations" @default.
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• W2103494397 cites W1966294794 @default.
• W2103494397 cites W1968207747 @default.
• W2103494397 cites W1976528127 @default.
• W2103494397 cites W1978512651 @default.
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• W2103494397 cites W1986143942 @default.
• W2103494397 cites W1994553203 @default.
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• W2103494397 cites W2002272386 @default.
• W2103494397 cites W2002287505 @default.
• W2103494397 cites W2002305721 @default.
• W2103494397 cites W2003546534 @default.
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• W2103494397 cites W2019814215 @default.
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• W2103494397 cites W2024726951 @default.
• W2103494397 cites W2029852478 @default.
• W2103494397 cites W2031371628 @default.
• W2103494397 cites W2037918022 @default.
• W2103494397 cites W2038098313 @default.
• W2103494397 cites W2043851684 @default.
• W2103494397 cites W2048104971 @default.
• W2103494397 cites W2049043712 @default.
• W2103494397 cites W2051603456 @default.
• W2103494397 cites W2055139912 @default.
• W2103494397 cites W2059282715 @default.
• W2103494397 cites W2060518376 @default.
• W2103494397 cites W2062248445 @default.
• W2103494397 cites W2064488813 @default.
• W2103494397 cites W2066363378 @default.
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• W2103494397 cites W2069466168 @default.
• W2103494397 cites W2069620701 @default.
• W2103494397 cites W2069869793 @default.
• W2103494397 cites W2073938212 @default.
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• W2103494397 doi "https://doi.org/10.1288/00005537-198207001-00001" @default.
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