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• W2103548276 abstract "A series of integrative and versatile broad-host-range promoter-probe vectors carrying reporter genes encoding green fluorescent protein (GFP), catechol 2,3-dioxygenase (XylE) or β -galactosidase (LacZ) were constructed for use in methanotrophs. These vectors facilitated the measurement of in vivo promoter activity in methanotrophs under defined growth conditions. They were tested by constructing transcriptional fusions between the soluble methane monooxygenase (sMMO) σ 54 promoter or particulate methane monooxygenase (pMMO) σ 70 promoter from Methylococcus capsulatus and the reporter genes. Reporter gene activity was measured under high- and low-copper growth conditions and the data obtained closely reflected transcriptional regulation of the sMMO or pMMO operon, thus demonstrating the suitability of these vectors for assessing promoter activity in methanotrophs. When β -galactosidase expression was coupled with the fluorogenic substrate 4-methylumbelliferyl β - d -glucuronide it yielded a sensitive and powerful screening system for detecting cells expressing this reporter gene. These data were substantiated with independent experiments using RT-PCR and RNA dot-blot analysis." @default.
• W2103548276 created "2016-06-24" @default.
• W2103548276 creator A5036995835 @default.
• W2103548276 creator A5051787900 @default.
• W2103548276 date "2009-03-01" @default.
• W2103548276 modified "2023-10-11" @default.
• W2103548276 title "Development and validation of promoter-probe vectors for the study of methane monooxygenase gene expression in Methylococcus capsulatus Bath" @default.
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• W2103548276 doi "https://doi.org/10.1099/mic.0.021816-0" @default.
• W2103548276 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19246747" @default.
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